Impact of Aging and Diabetes Mellitus on the Expression of the Proliferating Cell Nuclear Antigen in Rat Testicular Tissue

Salama, Mohamed E.; Tsuji, Masaaki; Tamura, Masato; Kagawa, Shunsuke

doi:10.3109/01485019808987932

Cited by 24 publications

(14 citation statements)

References 38 publications

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“…Our studies suggest that hyperglycemia or insulin resistance may primarily induce testis atrophy, subsequently causing a reduction in spermatogenesis, which was more remarkably observed in diabetic rats than in non-diabetic rats. Salama et al previously reported that spermatogenesis was reduced in OLETF rats compared with LETO rats [25], although their report did not involve hormonal measurements as performed in the present study. Thus, we investigated gonadal dysfunction in OLETF rats by examining a variety of sex related hormones, SHBG, and parameters of insulin resistance, and clarified that hypogonadism in OLETF rats was more notably found in the reduction of spermatogenesis than in the reduction of serum testosterone levels, and that these dysfunctions were closely related to hyperglycemia.…”

Section: Discussioncontrasting

confidence: 50%

Gonadal Hormones and Gonadal Function in Type 2 Diabetes Model OLETF (Otsuka Long Evans Tokushima Fatty) Rats

2005

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Abstract. Gonadal functions, with special reference to blood levels of sex-related markers such as 17b-estradiol (E2), free testosterone (free Te) and lutenizing hormone (LH), were examined in male OLETF (Otsuka Long Evans Tokushima Fatty) diabetic rats, a model of human type 2 diabetes mellitus. Male rats of the OLETF strain and male rats of the LETO strain, which act as a control of OLETF, both supplied by Otsuka Pharmaceutical Co., Ltd. (Tokushima, Japan), were periodically examined for blood levels of E2, free Te and LH at the age of 4, 5, 32, 40 and 64 weeks. The weight of the testis, the number of sperm contained within and histological findings of the testis were comparatively studied in both strains. Glucose and insulin (IRI) at fasting were examined to evaluate the homeostasis model assessment (HOMA) index. In order to investigate any sex hormone imbalance, sex hormone-binding globulin (SHBG) was measured by a dextrancharcoal assay. All of the OLETF rats became diabetic at the age of 32 weeks. There were no significant differences between OLETF and LETO rats regarding free Te, E2, LH or SHBG during the observation period from 4 to 64 weeks. Testis weight was significantly decreased in OLETF rats at 32 and 64 weeks and sperm counts at 64 weeks of age were also significantly decreased. Histologically, there was seminiferous tubule atrophy in the OLETF rats at 64 weeks of age. A significant negative correlation between testis weight and fasting blood glucose, as well as HOMA index, was observed in OLETF rats. In male diabetic OLETF rats, with a variety of hypogonadisms such as atrophy of the testis and low sperm count, the serum levels of E2, free Te, LH and SHBG were normally preserved. [5,6]. From about 20 weeks of age, OLETF rats begin to develop diabetes accompanied by obesity, and almost all of the rats develop diabetes by the age of 30 weeks. This study was intended to make clear, whether sexual dysfunctions, if any, occur in diabetic OLETF rats by examining serum levels of sex-related hormones and sex hormone-binding globulin (SHBG), as well as testis findings. SHBG is a factor affecting sex hormone activities by binding both testosterone and 17b-estradiol (E2), with a higher affinity for the former [7,8]. Materials and Methods AnimalsBoth OLETF rats and their controls, named LETO (Long-Evans Tokushima Otsuka) rats, which are nondiabetic, were supplied by Otsuka Pharmaceutical Co., Ltd. (Tokushima, Japan). OLETF rats comprising 4, 5, 32, 40 and 64 weeks of age and control LETO rats corresponding to each OLETF age were studied. After

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Section: Discussioncontrasting

confidence: 50%

Gonadal Hormones and Gonadal Function in Type 2 Diabetes Model OLETF (Otsuka Long Evans Tokushima Fatty) Rats

2005

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“…However the signal density of positive cells was significantly lower in diabetic group. Salama et al, (1998) examined the impact of aging and non-insulin-dependent diabetes mellitus on the expression of the PCNA in rat testicular tissue, and they found that older animals showed the fewest number of immunostained basal germ cells in the seminiferous tubuli (Salama et al, 1998). Also in diabetic rats, the PCNA index was decreased in the dizbetic groups.…”

Section: Discussionmentioning

confidence: 99%

Effects of experimental diabetes on testis proliferations and apoptosis in rats

AKTAS¹,

KANTER²,

ERBOĞA³

et al. 2011

jecm

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This work aimed to investigate the role of diabetes on cell proliferation, and apoptosis in testis. The rats were randomly allotted into one of two experimental groups: control and diabetic group; each group contain 10 animals. Diabetes was induced by a single intraperitoneal injection of STZ (50 mg/kg). Testicular damage was examined by using hematoxylin and eosin, immunohistochemical staining of proliferating cell nuclear antigen (PCNA), and apoptosis was determined by terminal-deoxynucleotidyl-transferase mediated dUTP nick end labeling (TUNEL). Potential disorders associated with seminiferous tubular sperm formation were evaluated using the Johnsen score. The mean seminiferous tubule diameter (MSTD) and mean testicular biopsy score (MTBS) values were significantly decreased in diabetic group was compared to the control group. Our data indicate a significant reduction in the expression of PCNA and an enhancement in the activity of TUNEL in testis tissues of the diabetic group. The effects of diabetes on spermatogenesis can be clearly detected as a testicular cell death and decrease in MTBS, MSTD, and PCNA expression.

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“…Salama et al [42] conducted an investigation to evaluate the expression of proliferating PCNA, which is one of the important markers of cell proliferation, in testicular tissue with aging and progress of DM. Both aging and DM have depressing effects on the expression of PCNA in testicular tissue.…”

Section: Diabetes Mellitusmentioning

confidence: 99%

Recent Advances in Clinicaivmolecular Andrology

Hafez¹

1998

Archives of Andrology

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Impact of Aging and Diabetes Mellitus on the Expression of the Proliferating Cell Nuclear Antigen in Rat Testicular Tissue

Cited by 24 publications

References 38 publications

Gonadal Hormones and Gonadal Function in Type 2 Diabetes Model OLETF (Otsuka Long Evans Tokushima Fatty) Rats

Gonadal Hormones and Gonadal Function in Type 2 Diabetes Model OLETF (Otsuka Long Evans Tokushima Fatty) Rats

Effects of experimental diabetes on testis proliferations and apoptosis in rats

Recent Advances in Clinicaivmolecular Andrology

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