Radio-protective effect of cinnamic acid, a phenolic phytochemical, on genomic instability induced by X-rays in human blood lymphocytes in vitro

Çinkılıç, Nilüfer; Tüzün, Ece; Çetintaş, Sibel Kahraman; Vatan, Özgür; Yılmaz, Dilek Demirezen; Çavaş, Tolga; Tunç, Sema; Özkan, Lütfi; Bilaloĝlu, Rahmi

doi:10.1016/j.mrgentox.2014.04.025

Cited by 17 publications

(10 citation statements)

References 33 publications

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“…In support to our data, Cinkilic et al [47] show that cinnamic acid protects against radiation-induced genomic instability (1 or 2 Gy) in normal human blood lymphocytes. Nonetheless, it has been recognized that carnauba wax did not show mutagenic effects on S. cerevisiae and several S. typhimurium strains [48].…”

Section: Discussionsupporting

confidence: 93%

p-Methoxycinnamic Acid Diesters Lower Dyslipidemia, Liver Oxidative Stress and Toxicity in High-Fat Diet Fed Mice and Human Peripheral Blood Lymphocytes

et al. 2020

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The pursuit of cholesterol lowering natural products with less side effects is needed for controlling dyslipidemia and reducing the increasing toll of cardiovascular diseases that are associated with morbidity and mortality worldwide. The present study aimed at the examining effects of p-methoxycinnamic acid diesters (PCO-C) from carnauba (Copernicia prunifera)-derived wax on cytotoxic, genotoxic responses in vitro and on dyslipidemia and liver oxidative stress in vivo, utilizing high-fat diet (HFD) chronically fed Swiss mice. In addition, we evaluated the effect of PCO-C on the expression of key cholesterol metabolism-related genes, as well as the structural interactions between PCO-C and lecithin-cholesterol acyl transferase (LCAT) in silico. Oral treatment with PCO-C was able to reduce total serum cholesterol and low-density lipoprotein (LDL) levels following HFD. In addition, PCO-C reduced excessive weight gain and lipid peroxidation, and increased the gene expression of LCAT following HFD. Furthermore, the high affinity of the studied compound (ΔG: −8.78 Kcal/mol) towards the active sites of mutant LCAT owing to hydrophobic and van der Waals interactions was confirmed using bioinformatics. PCO-C showed no evidence of renal and hepatic toxicity, unlike simvastatin, that elevated aspartate aminotransferase (AST) levels, a marker of liver dysfunction. Finally, PCO-C showed no cytotoxicity or genotoxicity towards human peripheral blood lymphocytes in vitro. Our results suggest that PCO-C exerts hypocholesterolemic effects. The safety of PCO-C in the toxicological tests performed and the reports of its beneficial biological effects render this a promising compound for the development of new cholesterol-lowering therapeutics to control dyslipidemia. More work is needed for further elucidating PCO-C role on lipid metabolism to support future clinical studies.

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Section: Discussionsupporting

confidence: 93%

p-Methoxycinnamic Acid Diesters Lower Dyslipidemia, Liver Oxidative Stress and Toxicity in High-Fat Diet Fed Mice and Human Peripheral Blood Lymphocytes

et al. 2020

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“…Cinnamic acid is a phenolic substance obtained from cinnamon oil, and has been shown to have antioxidant properties. Cinkilic et al [ 21 ] investigated the radioprotective effects of cinnamic acid against X-ray-induced genomic instability in human lymphocytes. They found that cinnamic acid-treated lymphocytes had a significant decrease in DNA DSBs (range from 16 to 55% reduction) compared to controls [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…Cinkilic et al [ 21 ] investigated the radioprotective effects of cinnamic acid against X-ray-induced genomic instability in human lymphocytes. They found that cinnamic acid-treated lymphocytes had a significant decrease in DNA DSBs (range from 16 to 55% reduction) compared to controls [ 21 ]. Pretreatment with cinnamic acid also reduced total genetic damage [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…They found that cinnamic acid-treated lymphocytes had a significant decrease in DNA DSBs (range from 16 to 55% reduction) compared to controls [ 21 ]. Pretreatment with cinnamic acid also reduced total genetic damage [ 21 ]. Cinnamic acid alone did not increase DSBs or other DNA damage, suggesting it is not genotoxic [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…Pretreatment with cinnamic acid also reduced total genetic damage [ 21 ]. Cinnamic acid alone did not increase DSBs or other DNA damage, suggesting it is not genotoxic [ 21 ]. The authors found that cinnamic acid decreased DNA damage induced by irradiation with X-rays by reducing the intracellular ROS level through its free-radical scavenging properties [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

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Radioprotective agents to prevent cellular damage due to ionizing radiation

et al. 2017

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Medical imaging has become a central component of patient care to ensure early and accurate diagnosis. Unfortunately, many imaging modalities use ionizing radiation to generate images. Ionizing radiation even in low doses can cause direct DNA damage and generate reactive oxygen species and free radicals, leading to DNA, protein, and lipid membrane damage. This cell damage can lead to apoptosis, necrosis, teratogenesis, or carcinogenesis. As many as 2% of cancers (and an associated 15,000 deaths annually) can be linked to computed tomography exposure alone. Radioprotective agents have been investigated using various models including cells, animals, and recently humans. The data suggest that radioprotective agents working through a variety of mechanisms have the potential to decrease free radical damage produced by ionizing radiation. Radioprotective agents may be useful as an adjunct to medical imaging to reduced patient morbidity and mortality due to ionizing radiation exposure. Some radioprotective agents can be found in high quantities in antioxidant rich foods, suggesting that a specific diet recommendation could be beneficial in radioprotection.

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