Radio-Frequency Sustainment of Laser Initiated, High-Pressure Air Constituent Plasmas

Akhtar, Khalid Pervaiz; Scharer, J.E.; Tysk, Shane; Denning, Mark

doi:10.1063/1.1638068

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…In regards to plasma technology, several atmospheric pressure plasma sources have been developed and evaluated for wide range of biomedical applications. The most common ways for generating atmospheric pressure non-equilibrium plasma based on different electrode configuration and mechanism of plasma formation include; atmospheric pressure glow discharge plasmas (Ben Gadri et al, 2000), DC/AC corona (Loeb, 1948;Nunez et al, 1993;Strobel et al, 1991), dielectric barrier discharge (from low frequency to several megahertz) (Indarto, 2008), floating barrier discharges (Fridman et al, 2006), inductively coupled plasma torches (Akhtar et al, 2003;Kovacic et al, 1985), plasma pencil (Laroussi et al, 2006), plasma needles (Li et al, 2010;Lu et al, 2009;Sakiyama and Graves, 2006;Stoffels et al, 2002Stoffels et al, , 2006, resistive barrier discharge (RBD) (Laroussi et al, 2002;Thiyagarajan et al, 2005), atmospheric pressure plasma jets (from dc to few gigahertz) (Sarani et al, 2010) and microwave driven discharges in the gigahertz range (Ferreira et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Induction of apoptosis in human myeloid leukemia cells by remote exposure of resistive barrier cold plasma

Thiyagarajan

Anderson

Gonzales

2013

Biotech & Bioengineering

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Cold atmospheric plasma (CAP), an ambient temperature ionized gas, is gaining extensive interest as a promising addition to anti-tumor therapy primarily due to the ability to generate and control delivery of electrons, ions, excited molecules, UV photons, and reactive species such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) to a specific site. The heterogeneous composition of CAP offers the opportunity to mediate several signaling pathways that regulate tumor cells. Consequently, the array of CAP generated products has limited the identification of the mechanisms of action on tumor cells. The aim of this work is to assess the cell death response of human myeloid leukemia cells by remote exposure to CAP generated RNS by utilizing a novel resistive barrier discharge system that primarily produces RNS. The effect of variable treatments of CAP generated RNS was tested in THP-1 cell (human monocytic leukemia cell line), a model for hematological malignancy. The number of viable cells was evaluated with erythrosine-B staining, while apoptosis and necrosis was assessed by endonuclease cleavage observed by agarose gel electrophoresis and detection of cells with the exclusionary dye propidium iodide and fluorescently labeled annexin-V by flow cytometry and fluorescent microscopy. Our observations indicate that treatment dosage levels of 45 s of exposure to CAP emitted RNS-induced apoptotic cell death and for higher dosage conditions of ≥50 s of exposure to CAP induced necrosis. Overall the results suggest that CAP emitted RNS play a significant role in the anti-tumor potential of CAP.

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