Characterization of Portable Resistive Barrier Plasma Jet and Its Direct and Indirect Treatment for Antibiotic Resistant Bacteria and THP-1 Leukemia Cancer Cells

Thiyagarajan, M.; Sarani, Abdollah; Gonzalez, Xavier F.

doi:10.1109/tps.2012.2222391

Cited by 18 publications

(8 citation statements)

References 63 publications

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“…RNS play contradictory roles by promoting cellular viability through homeostatic regulation of physiologic process or causing cell death with nitrosative stress that impairs the function of cells (Nathan, ). Cellular treatment by CAP has shown similar contradictory responses in enhancing and decreasing cell viability (Thiyagarajan et al, ). RBJ plasma discharged RNS treatment of THP‐1 cells demonstrated immediate decreases in viability following a 45 s dosage (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Cold atmospheric plasma (CAP) in general is plasma generated at atmospheric pressure and its rotational temperatures (gas temperatures) are close to ambient temperatures. CAP generates a variety of ions, free radicals, excited atoms and molecules, UV photons, and reactive species such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) close to room temperature (Pozebon et al, ; Thiyagarajan et al, ). Due to these remarkable properties, a myriad of possibilities are presented as an independent anti‐tumor therapy or part of a combination therapy.…”

Section: Introductionmentioning

confidence: 99%

“…Direct exposure of plasma involves exposure of plasma directly on to a target treatment surface. The indirect plasma exposure involves exposure of only the plasma effluent such as ROS and RNS generated by the plasma instead of the plasma itself, thus eliminating the effect of any possible UV radiation produced by plasma in the case of direct plasma exposure (Plimpton et al, ; Thiyagarajan et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Induction of apoptosis in human myeloid leukemia cells by remote exposure of resistive barrier cold plasma

Thiyagarajan

Anderson

Gonzales

2013

Biotech & Bioengineering

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Cold atmospheric plasma (CAP), an ambient temperature ionized gas, is gaining extensive interest as a promising addition to anti-tumor therapy primarily due to the ability to generate and control delivery of electrons, ions, excited molecules, UV photons, and reactive species such as reactive oxygen species (ROS) and reactive nitrogen species (RNS) to a specific site. The heterogeneous composition of CAP offers the opportunity to mediate several signaling pathways that regulate tumor cells. Consequently, the array of CAP generated products has limited the identification of the mechanisms of action on tumor cells. The aim of this work is to assess the cell death response of human myeloid leukemia cells by remote exposure to CAP generated RNS by utilizing a novel resistive barrier discharge system that primarily produces RNS. The effect of variable treatments of CAP generated RNS was tested in THP-1 cell (human monocytic leukemia cell line), a model for hematological malignancy. The number of viable cells was evaluated with erythrosine-B staining, while apoptosis and necrosis was assessed by endonuclease cleavage observed by agarose gel electrophoresis and detection of cells with the exclusionary dye propidium iodide and fluorescently labeled annexin-V by flow cytometry and fluorescent microscopy. Our observations indicate that treatment dosage levels of 45 s of exposure to CAP emitted RNS-induced apoptotic cell death and for higher dosage conditions of ≥50 s of exposure to CAP induced necrosis. Overall the results suggest that CAP emitted RNS play a significant role in the anti-tumor potential of CAP.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Induction of apoptosis in human myeloid leukemia cells by remote exposure of resistive barrier cold plasma

Thiyagarajan

Anderson

Gonzales

2013

Biotech & Bioengineering

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“…Current literature points to short‐lived species emanating from the discharge reaching the treatment site at distances no more than ∼1 cm (Maisch et al, 2012; Shimizu et al, 2012). Experimental detection at longer distances is somewhat limited, with known work concentrating solely on nitric oxide detection (Thiyagarajan et al, 2012). It is more likely that the trapped radicals are generated by secondary reactions of the species formed in the discharge.…”

Section: Discussionmentioning

confidence: 99%

Remote delivery of hydroxyl radicals via secondary chemistry of a nonthermal plasma effluent

Plimpton

Gołkowski

Mitchell

et al. 2013

Biotech & Bioengineering

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Electron paramagnetic resonance spectroscopy is used to observe hydroxyl radicals produced by an atmospheric pressure nonthermal plasma device at distances greater than 1 m from the discharge. The plasma device is an indirect treatment setup with closed loop airflow and hydrogen peroxide additives that is effective in deactivating bacteria on time scales of seconds. The generation of the detected hydroxyl radicals is shown to occur in secondary chemical processes near the point of delivery of the plasma treated air stream. The production of hydroxyl radicals is correlated with humidity of the air stream and ability to lyse bacterial membranes. The overall mechanisms of bacteria inactivation are found to be a combinatorial effect of effluent species. The results indicate the feasibility of selective plasma induced free radical delivery for biomedical applications even in the case of short-lived species like the hydroxyl radical.

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“…Biochemical mechanisms of plasma interaction with mammalian and other types of cells are considered. 39,240,[257][258][259] Differences between biochemical mechanisms of plasma interaction with mammalian cells and bacteria are discussed based on existing experimental data. Different effects on mammalian cell membranes are presented and analyzed.…”

Section: J Biochemical Mechanisms Of Plasma Interaction With Mammalimentioning

confidence: 99%

Review of Major Directions in Non-Equilibrium Atmospheric Plasma Treatments in Medical, Biological, and Bioengineering Applications

Han

2013

Plasma Med

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Plasma Medicine is the newest and rapidly expanding area of engineering medicine and bioengineering focused on direct applications of plasma for treatment of different diseases, blood coagulation control, wound management, and wound healing, as well as improving patient care through sterilization, medical implants, biomaterial engineering, and tissue engineering. The number of medical engineering professionals, researchers, upper undergraduate, and graduate university students involved today in plasma medicine is already large and growing; there is also an exponentially growing number of publications in this new field. All these students and professionals need the ability to find these publications to aid them in getting started and to advance in their research in plasma medicine. This determines the main purpose of this review, focused on, first of all, summarizing the major directions of fundamental plasma medicine and providing an extensive guide to specific diseases with current plasma-bioengineering solutions, as well as providing a relevant, up-to-date bibliography.

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Characterization of Portable Resistive Barrier Plasma Jet and Its Direct and Indirect Treatment for Antibiotic Resistant Bacteria and THP-1 Leukemia Cancer Cells

Cited by 18 publications

References 63 publications

Induction of apoptosis in human myeloid leukemia cells by remote exposure of resistive barrier cold plasma

Induction of apoptosis in human myeloid leukemia cells by remote exposure of resistive barrier cold plasma

Remote delivery of hydroxyl radicals via secondary chemistry of a nonthermal plasma effluent

Review of Major Directions in Non-Equilibrium Atmospheric Plasma Treatments in Medical, Biological, and Bioengineering Applications

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