Radical Scavenging Capacity in Human Skin before and after Vitamin C Uptake: An In Vivo Feasibility Study Using Electron Paramagnetic Resonance Spectroscopy

Lauer, Anna-Christina; Groth, Norbert; Haag, Stefan; Darvin, Maxim E.; Lademann, Jürgen; Meinke, Martina C.

doi:10.1038/jid.2012.420

Cited by 14 publications

(12 citation statements)

References 13 publications

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“…Thereby, the decrease of the signal intensity of the spin probe TEMPO was measured over time. The placebo group showed no alteration in their rate constant during the entire study [54]. In addition to these results, different vitamin C doses (dose 1: 100 mg/day; dose 2: 180 mg/day) were compared for their efficiency [53].…”

Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 96%

“…A comparison of the results of the recent studies on hydrophilic and lipophilic antioxidants [38,[53][54][55] clearly showed that after an oral intake of water-soluble antioxidants, the antioxidant potential of the skin increased within only four weeks, whereas lipophilic antioxidants needed eight weeks to become fully effective. An enrichment of the skin with carotenoids via an oral supplementation for eight weeks increased the free radical scavenging properties of the skin fairly well [38].…”

Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 99%

“…In one of the recent studies, the radical scavenging capacity of the human skin on the inner right forearm was examined on the baseline and four weeks after supplementation in response to supplementation with moderate vitamin C doses [53][54][55]. A total number of 33 healthy volunteers were given either supplements with vitamin C, a chokeberry peel extract (Aronia melanocarpa), or a placebo for four weeks [54]. The amount of supplementation corresponded to 125 mg of calcium ascorbate per day or 800 mg of Aronia melanocarpa combined with 40 mg of calcium ascorbate.…”

Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 99%

“…No significant increase in the carotenoid concentration was detected subsequent to supplementation with pure vitamin C and the mixed preparations after four weeks using resonance Raman spectroscopy. Long-term effects were not assessed as the period of study was too short [53][54][55].…”

Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 99%

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Determination of the Antioxidant Status of the Skin by In Vivo-Electron Paramagnetic Resonance (EPR) Spectroscopy

et al. 2015

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Organisms produce free radicals which are essential for various metabolic processes (enzymatic oxidation, cellular respiration, signaling). Antioxidants are important chemical compounds that specifically prevent the oxidation of substances by scavenging radicals, especially reactive oxygen species (ROS). Made up of one or two unpaired electrons, ROS are free radicals that are highly reactive and can attack other metabolites. By using electron paramagnetic resonance (EPR) spectroscopy, it is possible to measure paramagnetic substances such as free radicals. Therefore the dermal antioxidant activity can be determined by applying semi-stable radicals onto the skin and measuring the antioxidant-induced radical scavenging activity in the skin. In recent years, EPR has been OPEN ACCESS Cosmetics 2015, 2 287 developed as a spectroscopic method for determining the antioxidant status in vivo. Several studies have shown that an additional uptake of dietary supplements, such as carotenoids or vitamin C in physiological concentrations, provide a protective effect against free radicals. Using the EPR technique it could be demonstrated that the radical production in stress situations, such as irradiation with infrared and visible light, was reduced with time. However, not only the oral uptake of antioxidants, but also the topical application of antioxidants, e.g., a hyperforin-rich cream, is very useful against the development of oxidative stress. Regular application of a hyperforin-rich cream reduced radical formation. The skin lipids, which are very important for the barrier function of the skin, were also stabilized.

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Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 96%

Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 99%

Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 99%

Section: Effect Of Orally Ingested Antioxidantsmentioning

confidence: 99%

See 2 more Smart Citations

Determination of the Antioxidant Status of the Skin by In Vivo-Electron Paramagnetic Resonance (EPR) Spectroscopy

et al. 2015

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“…Recently is was published in a letter by Lauer et al [1] that a low-dose intake of vitamin C could be measured by electron paramagnetic resonance (EPR) spectroscopy. In this paper, the study is presented in more detail.…”

Section: Introductionmentioning

confidence: 99%

Dose-Dependent Vitamin C Uptake and Radical Scavenging Activity in Human Skin Measured with in vivo Electron Paramagnetic Resonance Spectroscopy

Lauer

Groth²,

Haag

et al. 2013

Skin Pharmacol Physiol

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Vitamin C is a potent radical scavenger and a physiological part of the antioxidant system in human skin. The aim of this study was to measure changes in the radical-scavenging activity of human skin in vivo due to supplementation with different doses of vitamin C and at different time points. Therefore, 33 volunteers were supplemented with vitamin C or placebo for 4 weeks. The skin radical-scavenging activity was measured with electron paramagnetic resonance spectroscopy. After 4 weeks, the intake of 100 mg vitamin C/day resulted in a significant increase in the radical-scavenging activity by 22%. Intake of 180 mg/day even resulted in a significant increase of 37%. No changes were found in the placebo group. A part of the study population was additionally measured after 2 weeks: in this group radical scavenging had already reached maximal activity after 2 weeks. In conclusion, orally administered vitamin C increases the radical-scavenging activity of the skin. The effect occurs fast and is enhanced with higher doses of vitamin C.

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Spectroscopic biofeedback on cutaneous carotenoids: A powerful tool for primary prevention in advanced age

Gehlich

Koch²,

Köcher³

et al. 2023

Journal of Biophotonics

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Radical Scavenging Capacity in Human Skin before and after Vitamin C Uptake: An In Vivo Feasibility Study Using Electron Paramagnetic Resonance Spectroscopy

Cited by 14 publications

References 13 publications

Determination of the Antioxidant Status of the Skin by In Vivo-Electron Paramagnetic Resonance (EPR) Spectroscopy

Determination of the Antioxidant Status of the Skin by In Vivo-Electron Paramagnetic Resonance (EPR) Spectroscopy

Dose-Dependent Vitamin C Uptake and Radical Scavenging Activity in Human Skin Measured with in vivo Electron Paramagnetic Resonance Spectroscopy

Spectroscopic biofeedback on cutaneous carotenoids: A powerful tool for primary prevention in advanced age

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