Radiation-induced reactive oxygen species partially assemble neutrophil NADPH oxidase

Owusu, Stephenson Boakye; Hudik, Elodie; Férard, Céline; Dupré-Crochet, Sophie; Addison, Eric C.D.K.; Preko, Kwasi; Bizouarn, Tania; Houée-Levin⊥, Chantal; Baciou, Laura

doi:10.1016/j.freeradbiomed.2020.12.233

Cited by 10 publications

(12 citation statements)

References 63 publications

(68 reference statements)

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“…When cells are submitted to irradiation, cyt b 558 is re-localized at the plasma membrane as observed already for a very low dose. In our previous work performed on irradiated neutrophils [ 8 ], we observed a pre-assembly of the NADPH oxidase complex. We therefore investigated if PLB cell irradiation similarly induced a partially pre-assembled NAPH oxidase.…”

Section: Resultsmentioning

confidence: 99%

“…The survival curves of irradiated and non-irradiated PLB-985 cells are shown in Figure 1 . Considering the survival curve of neutrophils (sham) determined in previous work [ 8 ], it was surprising to observe that PLB-985 cell deaths started in the course of time much longer than neutrophils and, in addition, with a different pattern. The course of time of irradiated PLB-985 cells appeared to be different depending on the irradiation dose.…”

Section: Resultsmentioning

confidence: 99%

“…We showed that the decrease of survival PLB-985 cells for sham appeared only after 3 h. The 0.2 Gy-irradiated cells showed almost no changes compared to sham. This course of PLB-985 cell death is different from that of neutrophils, for which even the non-irradiated cells decline to about 85 percent within the first 30 min [ 8 ], indicating that PLB-985 cells survive much longer (in vitro in PBS). The cell behavior suggests that PLB-985 cells are more resistant to environment variations (maybe related to the absence of isolation procedures from donor blood pocket necessary for neutrophils).…”

Section: Discussionmentioning

confidence: 99%

“…Several studies on the effect of high doses (>50 Gy) on white blood cells have been reported but with many contradictions regarding the effects of irradiation on neutrophil functions, such as superoxide production, bactericidal activity and chemotaxis response [ 3 , 4 , 5 , 6 , 7 ]. We have shown in a recent work that a critical parameter that should be taken into account when the effect of irradiation is studied is the number of surviving cells [ 8 ]. We investigated the relationship between the dose of irradiation at the cellular level and the activation of the NADPH oxidase complex at the molecular level.…”

Section: Introductionmentioning

confidence: 99%

“…We investigated the relationship between the dose of irradiation at the cellular level and the activation of the NADPH oxidase complex at the molecular level. Our results indicated that radiation dose above 25 Gy acts as neutrophil stimuli, leading to a priming state of the NADPH oxidase complex and enhancement of respiratory burst due to NADPH oxidase activity [ 8 ]. This important observation could not be noted in a previous work since the purified components of NADPH oxidase, either individually or in the course of assembly, were subjected to exogenous ROS [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Accumulation of Cytochrome b558 at the Plasma Membrane: Hallmark of Oxidative Stress in Phagocytic Cells

Owusu

Dupré-Crochet

Bizouarn

et al. 2022

IJMS

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Neutrophils play a very key role in the human immune defense against pathogenic infections. The predominant players in this role during the activation of neutrophils are the release of cytotoxic agents stored in the granules and secretory vesicles and the massive production of reactive oxygen species (ROS) initiated by the enzyme NADPH oxidase. In addition, in living organisms, cells are continuously exposed to endogenous (inflammations, elevated neutrophil presence in the vicinity) and exogenous ROS at low and moderate levels (travels by plane, radiotherapy, space irradiation, blood banking, etc.). To study these effects, we used ROS induced by gamma radiation from low (0.2 Gy) to high (25 Gy) dose levels on PLB-985 cells from a myeloid cell line differentiated to neutrophil-like cells that are considered a good alternative to neutrophils. We determined a much longer lifetime of PLB-985 cells than that of neutrophils, which, as expected, decreased by increasing the irradiation dose. In the absence of any secondary stimulus, a very low production of ROS is detected with no significant difference between irradiated and non-irradiated cells. However, in phagocytosing cells, irradiation doses above 2 Gy enhanced oxidative burst in PLB-985 cells. Whatever the irradiation dose, NADPH oxidase devoid of its cytosolic regulatory units is observed at the plasma membrane in irradiated PLB-985 cells. This result is different from that observed for irradiated neutrophils in which irradiation also induced a translocation of regulatory subunits suggesting that the signal transduction mechanism or pathway operate differently in both cells.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Accumulation of Cytochrome b558 at the Plasma Membrane: Hallmark of Oxidative Stress in Phagocytic Cells

Owusu

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Bizouarn

et al. 2022

IJMS

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Spatiotemporal Catalytic Nanozymes Microneedle Patches with Opposite Properties for Wound Management

Shan

Zhang

Wang

et al. 2023

Small

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Reactive oxygen species (ROS)‐mediated biological catalysis involves serial programmed enzymatic reactions and plays an important part against infectious diseases; while the spatiotemporal control of catalytic treatment to break the limitations of the disease microenvironment is challenging. Here, a novel spatiotemporal catalytic microneedles patch (CMSP‐MNs) integrated with dual‐effective Cu2MoS4 (CMS) and polydopamine (PDA) nanoparticles (NPs) for breaking microenvironment restrictions to treat wound infections is designed. Since CMS NPs are loaded in the needles, CMSP‐MNs can catalytically generate diverse ROS to cause effective bacterial inactivation during bacterial infection process. Besides, PDA NPs are encapsulated in the backing layer, which facilitate ROS elimination and oxygen production for solving hypoxic problems in wound microenvironment and alleviating the expression of inflammatory factors during the inflammation process. Based on these features, it is demonstrated through cell and animal experiments that these nanozymes‐integrated MNs patches can realize selective regulation of ROS level with bacterial inactivation and inflammatory treatment, resulting in minimized side effects of over‐production ROS and effective anti‐infected treatment. It is believed that the presented MNs can provide a new therapeutic strategy with spatiotemporal adjustable catalytic properties in biomedical areas.

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Amplifying protection against acute lung injury: Targeting both inflammasome and cGAS-STING pathway by drug pair

Li,

Dong,

Yao

et al. 2024

Chinese Herbal Medicines

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Radiation-induced reactive oxygen species partially assemble neutrophil NADPH oxidase

Cited by 10 publications

References 63 publications

Accumulation of Cytochrome b558 at the Plasma Membrane: Hallmark of Oxidative Stress in Phagocytic Cells

Accumulation of Cytochrome b558 at the Plasma Membrane: Hallmark of Oxidative Stress in Phagocytic Cells

Spatiotemporal Catalytic Nanozymes Microneedle Patches with Opposite Properties for Wound Management

Amplifying protection against acute lung injury: Targeting both inflammasome and cGAS-STING pathway by drug pair

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