Radiation-Induced Micronucleus Frequency in Peripheral Blood Lymphocytes is Correlated with Normal Tissue Damage in Patients with Cervical Carcinoma Undergoing Radiotherapy

Abstract

In an effort to find a test to predict the response of normal tissue to radiotherapy, the lymphocyte micronucleus assay was used on blood samples from patients with cervical carcinoma. Peripheral blood samples from 55 patients with advanced-stage (II B-IV B) cervical carcinoma were obtained before radiotherapy. The patients were treated with external-beam radiotherapy followed by high-dose-rate brachytherapy. Acute and late normal tissue reactions were scored and correlated with the micronucleus frequency in lymphocytes after irradiation with 4 Gy in vitro. Great interindividual variability was observed in the radiation-induced lymphocyte micronucleus frequency, especially at 4 Gy. The mean number of micronuclei per 100 binucleated cells in cells irradiated with 4 Gy in vitro was significantly higher in samples from patients who suffered from acute and/or late normal tissue reactions than in those from patients with no reactions (51.0 +/- 17.7 and 29.6 +/- 10.1, respectively). A significant correlation was also found between the micronucleus frequency at 4 Gy and the severity of acute reactions and late reactions. However, the overlap between the micronucleus frequencies of patients with high-grade late normal tissue reactions and low-grade reactions is too great to recommend the micronucleus assay in its present form for routine clinical application.

Effect of Henna (Lawsonia inermis) extract on the immunity and survival of common carp, Cyprinus carpio infected with Aeromonas hydrophila

“…This increment might be in part due to the activation of the haemopoietic tissues by L. inermis as previously reported in rainbow trout following in vitro treatment of macrophages with Glycyrrhiza glabra extract (Jang et al 1995). Similar findings were obtained by Barrett (2003) and Widel et al (2003), who reported that Echinacea preparations influenced the leukocyte count. Likewise, dietary garlic exhibited an increased level of WBC, lymphocytes, monocytes and neutrophils in C. carpio (Iranloye 2002).…”

Amelioration of Radiation-Induced Hematological and Biochemical Alterations in Swiss Albino Mice by Panax ginseng Extract

“…The extract of P ginseng had been reported to ameliorate radiation-induced damage in mice. 34 These reports demonstrate the feasibility of using the extract of this plant to reduce the radiation injuries on peripheral blood.…”

Biomonitoring of the cytogenetic effect of antitumor therapy by means of micronucleus assay in exfoliated epithelial cells

“…In buccal mucosa 8.8 ‰ cell with MN were observed after the dose of 48 Gy, and only 7.6 ‰ after 68 Gy [26]. This phenomenon was observed also in lymphocytes of head and neck and cervix cancer patients during radiotherapy where the fre quencies of micronuclei increased during the first half of therapy and declined thereafter, reaching, in some patients, values below the pre treatment level [26,31]. In all mentioned cases no attention was paid to outcome of the therapy.…”