Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane (Saccharum officinarum L.)

Nikam, Ashok A.; Devarumath, R. M.; Ahuja, Akash; Babu, Harinath; Shitole, M. G.; Suprasanna, Penna

doi:10.1016/j.cj.2014.09.002

Cited by 47 publications

(15 citation statements)

References 35 publications

(43 reference statements)

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“…The use of mutagen often causes cell damage and thus affects the ability of the putative mutant cell regeneration (Nikam et al 2015). The ability of each cell to regenerate shoots depends on the level of sensitivity of each cell.…”

Section: Discussionmentioning

confidence: 99%

Increasing Al-Tolerance of Sugarcane Using Ethyl Methane Sulphonate and In Vitro Selection in the Low pH Media

Purnamaningsih

Hutami

2016

HAYATI Journal of Biosciences

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a b s t r a c tIncreased production of sugarcane in Indonesia can be done with extensification sugarcane plantations which largely dominated by acidic upland red-yellow podzolic soil. High aluminium (Al) content and low pH of the soil can inhibit plant growth and development. Tolerant sugarcane in acid soil is the most efficient way, but the adaptive variety is still limited. In vitro culture technique can increase genetic variability to assemble new varieties through somaclonal variation combined with mutation using ethyl methane sulphonate (EMS). The new characters was directed by in vitro selection using AlCl 3 .6H 2 O with pH ¼ 4 as a component of selection for resistance to high aluminium. VMC 7616 and PS 862 varieties were used as materials. Mutation induced using EMS at concentrations of 0.1%, 0.3%, and 0.5% for 30, 60 and 120 minutes. Plantlets mutant obtained through callus formation, immersion callus in EMS, in vitro selection, and regeneration of callus. Result of study showed that the long immersion in the EMS solution caused greater damage to the cells, as indicated by the change in callus colour. Callus immersion time in EMS gave greater influence to regeneration compared to concentration of EMS. PS 862 had higher Al tolerance than VMC 7616. Rooting of shoot induced using indole-3-butyric acid (IBA) 3 mg/L.

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Section: Discussionmentioning

confidence: 99%

Increasing Al-Tolerance of Sugarcane Using Ethyl Methane Sulphonate and In Vitro Selection in the Low pH Media

Purnamaningsih

Hutami

2016

HAYATI Journal of Biosciences

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“…Proline is one of the major osmolytes regulating osmotic adjustment in plants exposed to osmotic stresses, and active accumulation of proline is associated with salinity tolerance in various plant species [5]. Proline contributes to membrane stability and mitigates the effect of NaCl on cell membrane disruption [39]. The proline content was also increased by irradiation in sweet potato [31], arabidopsis [20] and sugarcane [40].…”

Section: Discussionmentioning

confidence: 99%

Pretreatment with High-Dose Gamma Irradiation on Seeds Enhances the Tolerance of Sweet Osmanthus Seedlings to Salinity Stress

Geng

Zhang

Wang

et al. 2019

Forests

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The landscape application of sweet osmanthus (Osmanthus fragrans) with flower fragrance and high ornamental value is severely limited by salinity stress. Gamma irradiation applied to seeds enhanced their tolerance to salinity stress as reported in other plants. In this study, O. fragrans ‘Huangchuang Jingui’ seeds were pretreated with different doses of gamma irradiation, and tolerance of the seedlings germinated from the irradiated seeds to salinity stress and the changes of reactive oxygen species (ROS) production and ROS scavenging systems induced by gamma irradiation were observed. The results showed that seed pretreatment with different doses of gamma irradiation enhanced the tolerance of sweet osmanthus seedlings to salinity stress, and the positive effect induced by gamma irradiation was more remarkable with the increase of radiation dose (50–150 Gy). The pretreatment with high-dose irradiation decreased O2− production under salinity stress and mitigated the oxidative damage marked by a lower malondialdehyde (MDA) level, which could be related to the significant increase of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activities in the seedlings germinated from the irradiated seeds compared to the corresponding control seedlings. In addition, the accumulation of proline in the irradiated seedlings may contribute to enhancing their tolerance to salt stress by the osmotic adjustment. The study demonstrated the importance of regulating plant ROS balance under salt stress and provided a potential approach to improve the tolerance of sweet osmanthus to salt stress.

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“…Several sources of genetic variation are available to complement the genetic paucity of modern elite cultivars. Genetic diversity generated artificially through mutagenesis has proven a valuable resource for various crop species, as variants can be directly produced in commercial germplasm (Caldwell et al ., ; Mba, ; Nikam et al ., ; Gulfishan et al ., ; Çelik and Atak, ; Pando and Deza, ; Wang et al ., ; Tu Anh et al ., ). However, given that salt tolerance is most likely to arise from the concerted effects of numerous mechanisms, the potential to artificially create salt tolerant variants is surely limited.…”

Section: Harnessing the Genetic Diversity Of Exotic Germplasmmentioning

confidence: 98%

Salt stress under the scalpel – dissecting the genetics of salt tolerance

et al. 2019

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Summary Salt stress limits the productivity of crops grown under saline conditions, leading to substantial losses of yield in saline soils and under brackish and saline irrigation. Salt tolerant crops could alleviate these losses while both increasing irrigation opportunities and reducing agricultural demands on dwindling freshwater resources. However, despite significant efforts, progress towards this goal has been limited, largely because of the genetic complexity of salt tolerance for agronomically important yield‐related traits. Consequently, the focus is shifting to the study of traits that contribute to overall tolerance, thus breaking down salt tolerance into components that are more genetically tractable. Greater consideration of the plasticity of salt tolerance mechanisms throughout development and across environmental conditions furthers this dissection. The demand for more sophisticated and comprehensive methodologies is being met by parallel advances in high‐throughput phenotyping and sequencing technologies that are enabling the multivariate characterisation of vast germplasm resources. Alongside steady improvements in statistical genetics models, forward genetics approaches for elucidating salt tolerance mechanisms are gaining momentum. Subsequent quantitative trait locus and gene validation has also become more accessible, most recently through advanced techniques in molecular biology and genomic analysis, facilitating the translation of findings to the field. Besides fuelling the improvement of established crop species, this progress also facilitates the domestication of naturally salt tolerant orphan crops. Taken together, these advances herald a promising era of discovery for research into the genetics of salt tolerance in plants.

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Radiation-induced in vitro mutagenesis system for salt tolerance and other agronomic characters in sugarcane (Saccharum officinarum L.)

Cited by 47 publications

References 35 publications

Increasing Al-Tolerance of Sugarcane Using Ethyl Methane Sulphonate and In Vitro Selection in the Low pH Media

Increasing Al-Tolerance of Sugarcane Using Ethyl Methane Sulphonate and In Vitro Selection in the Low pH Media

Pretreatment with High-Dose Gamma Irradiation on Seeds Enhances the Tolerance of Sweet Osmanthus Seedlings to Salinity Stress

Salt stress under the scalpel – dissecting the genetics of salt tolerance

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