Radiation-induced changes of structural and functional properties of human hemoglobin

Szweda‐Lewandowska, Zofia; Puchała, Mieczysław; Osmulski, Paweł A.; Rosin, Janusz

doi:10.1007/bf01209722

Cited by 8 publications

(1 citation statement)

References 15 publications

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“…It is reported that Hb is converted to met-Hb or intramolecular-crosslinked Hb by the reaction with products of water radiolysis; mainly hydroxy radicals [15]. Carbonylation of Hb is an effective method of suppressing met-Hb formation.…”

Section: Stability Of Hb After Y-irradiationmentioning

confidence: 99%

Encapsulation of Hb into unsaturated lipid vesicles and γ‐ray polymerization

Sakai

Takeoka

Yokohama

et al. 1992

Polymers for Advanced Techs

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A carbonyl hemoglobin (HbCO) solution was stirred with a mixed powder of polymerizable 1,2-bis(2,4octadecadienoyl)-sn-glycero-3-phosp hocholine (DODPC), cholesterol and stearic acid ( 7 / 7 / 2 by mol). The mixture was extruded through polycarbonate membrane filters (final pore size = 0.2 p m 0). The average diameter of the resulting vesicles was 203 f 39 nm. The [Hb]l[Lipidl ratio (the weight ratio of Hb in vesicle to lipid) increased with the Hb concentration, and decreased with the NaCl concentration. A maximum [Hbll [lipid] ratio was observed at pH 6.9, which was the same as the isoelectric point of Hb. The vesicles were stabilized by y-irradiation (@CO) because the bilayer lipids bound each other to yield polyphospholipids. Denaturation of Hb by y-irradiation was not detected. These polyphospholipid vesicles encapsulating Hb were stable even against the freeze-thaw cycles and the freeze-drying procedure.

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Section: Stability Of Hb After Y-irradiationmentioning

confidence: 99%

Encapsulation of Hb into unsaturated lipid vesicles and γ‐ray polymerization

Sakai

Takeoka

Yokohama

et al. 1992

Polymers for Advanced Techs

View full text Add to dashboard Cite

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Radiolysis of radioimmunoconjugates. Reduction in antigen‐binding ability by α‐particle radiation

Larsen

Bruland

1995

Labelled Comp Radiopharmac

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The potential of delivering a high and localized radiation dose using α‐particle‐emitting radioimmunoconjugates (RIC's) is investigated as treatment for some types of cancer. High specific activity of the RIC may for some uses be desirable, allowing enrichment of the radionuclide on membrane antigens on target cells. Radiation exposure during the radiolabelling procedures may, however, reduce the antigen binding ability of RIC's of high specific activity. The influence of α‐particle dose on the cell binding fraction was therefore investigated for two monoclonal antibodies, i.e., TP‐1 F(ab')2 and TP‐3 IgG. Samples of 125I‐labelled MoAb were added different amounts of [211At]astatide to give accumulated doses ranging from 50 to 50,000 Gy. After the 211At had decayed 15 half‐lives, the cell binding fractions were measured. The results show that [211At] astatide activities up to 25 kBq/μl, corresponding to an accumulated α‐particle radiation dose of approximately 1,000 Gy, could be tolerated without significantly reducing the cell binding fraction, whereas higher doses gradually reduced the immunoreactivity. Although there may be differences in dose sensitivity of different radioimmunoconjugate due to differences in radionuclide conjugation methods, size of the antibody and structure of the antigen binding portion, the results presented here indicate that it is possible to produce radioimmunoconjugates of high specific activity with currently used methods of preparation. The potential problem of radiolysis should, however, be taken into consideration when planning procedures for preparation and use of α‐particle‐emitting radioimmunoconjugates.

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