Radiation Dose Limits for Bioanalytical X-ray Fluorescence Microscopy

Jones, Michael W. M.; Hare, Dominic J.; James, Simon; Jonge, Martin D. de; McColl, Gawain

doi:10.1021/acs.analchem.7b02817

Cited by 50 publications

(22 citation statements)

References 36 publications

(78 reference statements)

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“…In the latter, however, the exposed region was identified with the stronger ultrastructural damage and shrinking, thus suggesting its higher radio-sensitivity than the cryofixed sample. Interestingly, in line with Bedolla et al (2018), Jones et al (2017) reported that the sample's Ultralene film overlay was also damaged. Therefore, disentangling sample damage with that of the substrate is an additional challenge that must be tackled in order to provide a comprehensive overview of the interaction pattern in biological samples of different preparation protocols.…”

Section: Discussionmentioning

confidence: 60%

“…The height of the Si-H band included less than 5% of the maximum absorbance, which would be at the noise level for the cellular systems reported by Gianoncelli et al Needless to mention, the sample state plays an important role in radiation damage, and lyophilized samples have long been known to withstand radiation doses of the order of 10 7 Gy (Williams et al, 1993). Single cells were, in turn, presented morphologically intact upon substantially higher doses up to 10 10 Gy (Le Gros et al, 2016;Jones et al, 2017). Lombi et al (2011) discovered that XRF micro-tomography experiments induce the dislocation of chemical elements in fresh apical tissue samples of cowpea.…”

Section: Discussionmentioning

confidence: 91%

“…Specifically, in 3D reconstructions of Zn/Cu concentration, the extent to signal loss was directly proportional to the delivered dose, starting from 0.18 MGy. Jones et al (2017) reported on post-irradiation chemo-structural patterns in Caenorhabditis elegans samples of various preparation/hydration, all mapped by a hard XRF microprobe (15.6 keV energy). The authors concluded significant redistribution of labile ions of K/Ca.…”

Section: Discussionmentioning

confidence: 99%

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Soft X-ray induced radiation damage in thin freeze-dried brain samples studied by FTIR microscopy

Surowka¹,

Gianoncelli²,

Birarda³

et al. 2020

J Synchrotron Rad

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In order to push the spatial resolution limits to the nanoscale, synchrotron-based soft X-ray microscopy (XRM) experiments require higher radiation doses to be delivered to materials. Nevertheless, the associated radiation damage impacts on the integrity of delicate biological samples. Herein, the extent of soft X-ray radiation damage in popular thin freeze-dried brain tissue samples mounted onto Si3N4 membranes, as highlighted by Fourier transform infrared microscopy (FTIR), is reported. The freeze-dried tissue samples were found to be affected by general degradation of the vibrational architecture, though these effects were weaker than those observed in paraffin-embedded and hydrated systems reported in the literature. In addition, weak, reversible and specific features of the tissue–Si3N4 interaction could be identified for the first time upon routine soft X-ray exposures, further highlighting the complex interplay between the biological sample, its preparation protocol and X-ray probe.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Soft X-ray induced radiation damage in thin freeze-dried brain samples studied by FTIR microscopy

Surowka¹,

Gianoncelli²,

Birarda³

et al. 2020

J Synchrotron Rad

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show abstract

“…For samples sensitive to radiation damage, a cryogenic stream environment is available (Oxford Cryostream 700 Series), where the stream is directed downwards. It typically operates at À100 C and is effective in minimizing the influence of radiation damage in organisms such as Caenorhabditis elegans (Jones et al, 2017) and hydrated plant material (Jones, Kopittke et al, 2019). A jacket flow of dried facilitycompressed air is used to avoid the inherent noise, vibration and bulk of a local compressor.…”

Section: Sample Mounting and Environmentsmentioning

confidence: 99%

The XFM beamline at the Australian Synchrotron

et al. 2020

Self Cite

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The X-ray fluorescence microscopy (XFM) beamline is an in-vacuum undulator-based X-ray fluorescence (XRF) microprobe beamline at the 3 GeV Australian Synchrotron. The beamline delivers hard X-rays in the 4–27 keV energy range, permitting K emission to Cd and L and M emission for all other heavier elements. With a practical low-energy detection cut-off of approximately 1.5 keV, low-Z detection is constrained to Si, with Al detectable under favourable circumstances. The beamline has two scanning stations: a Kirkpatrick–Baez mirror microprobe, which produces a focal spot of 2 µm × 2 µm FWHM, and a large-area scanning `milliprobe', which has the beam size defined by slits. Energy-dispersive detector systems include the Maia 384, Vortex-EM and Vortex-ME3 for XRF measurement, and the EIGER2 X 1 Mpixel array detector for scanning X-ray diffraction microscopy measurements. The beamline uses event-mode data acquisition that eliminates detector system time overheads, and motion control overheads are significantly reduced through the application of an efficient raster scanning algorithm. The minimal overheads, in conjunction with short dwell times per pixel, have allowed XFM to establish techniques such as full spectroscopic XANES fluorescence imaging, XRF tomography, fly scanning ptychography and high-definition XRF imaging over large areas. XFM provides diverse analysis capabilities in the fields of medicine, biology, geology, materials science and cultural heritage. This paper discusses the beamline status, scientific showcases and future upgrades.

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“…First, it is important to note that the absorbed doses in SR-based studies of biological samples are extreme. Although radiation-induced damage can occur at doses of just a few grays (1 Gy = 1 J of absorbed radiation energy per 1 kg of absorbing tissue mass), the X-ray exposures in synchrotron-based studies reach the MGy (10 6 Gy) unit scale and higher [41,42]. By comparison, radiation doses from exposures to X-rays or gamma-rays in clinical procedures are below 1 Gy and are measured in units of cGy (10 −2 Gy) in external-beam radiation therapy, and in units of mGy (10 −3 Gy) in conventional diagnostic X-ray imaging and nuclear medicine procedures [43].…”

Section: Radiation-induced Damage In Biological Samplesmentioning

confidence: 99%

Probing Trace Elements in Human Tissues with Synchrotron Radiation

Gherase

Fleming

2019

Crystals

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For the past several decades, synchrotron radiation has been extensively used to measure the spatial distribution and chemical affinity of elements found in trace concentrations (<few µg/g) in animal and human tissues. Intense and highly focused (lateral size of several micrometers) X-ray beams combined with small steps of photon energy tuning (2–3 eV) of synchrotron radiation allowed X-ray fluorescence (XRF) and X-ray absorption spectroscopy (XAS) techniques to nondestructively and simultaneously detect trace elements as well as identify their chemical affinity and speciation in situ, respectively. Although limited by measurement time and radiation damage to the tissue, these techniques are commonly used to obtain two-dimensional and three-dimensional maps of several elements at synchrotron facilities around the world. The spatial distribution and chemistry of the trace elements obtained is then correlated to the targeted anatomical structures and to the biological functions (normal or pathological). For example, synchrotron-based in vitro studies of various human tissues showed significant differences between the normal and pathological distributions of metallic trace elements such as iron, zinc, copper, and lead in relation to human diseases ranging from Parkinson’s disease and cancer to osteoporosis and osteoarthritis. Current research effort is aimed at not only measuring the abnormal elemental distributions associated with various diseases, but also indicate or discover possible biological mechanisms that could explain such observations. While a number of studies confirmed and strengthened previous knowledge, others revealed or suggested new possible roles of trace elements or provided a more accurate spatial distribution in relation to the underlying histology. This area of research is at the intersection of several current fundamental and applied scientific inquiries such as metabolomics, medicine, biochemistry, toxicology, food science, health physics, and environmental and public health.

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Radiation Dose Limits for Bioanalytical X-ray Fluorescence Microscopy

Cited by 50 publications

References 36 publications

Soft X-ray induced radiation damage in thin freeze-dried brain samples studied by FTIR microscopy

Soft X-ray induced radiation damage in thin freeze-dried brain samples studied by FTIR microscopy

The XFM beamline at the Australian Synchrotron

Probing Trace Elements in Human Tissues with Synchrotron Radiation

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