Recent advances in aberration-correcting electron optics have made sub-Ångstrom imaging in transmission electron microscopy almost routine in both the broad beam and the scanning probe modes [1][2][3][4]. The desire to further improve the spatial resolution in electron microscopy is driven in large part by the need for increased sensitivity, image contrast [5] and atomic-resolution tomography [6]. In this Letter, we report on utilizing a new generation aberration-corrected microscope to form a highly coherent sub-50 pm electron probe at 300 kV and demonstrate that this probe is capable of resolving the 47 pm dumbbell spacing in a Ge 〈114〉 crystal.In scanning transmission electron microscopy (STEM), the size of the electron probe that is focused onto the specimen ultimately limits the spatial resolution. Apart from mechanical and electrical stability, the size of the probe is determined by the illumination half-angle α, residual coherent axial aberrations, and incoherent broadening due to partial temporal and partial spatial coherence given by the finite energy length and the finite size of the demagnified electron source, respectively.