Rabbits as models for impotence research

Bischoff, Erwin

doi:10.1038/sj.ijir.3900681

Cited by 13 publications

(12 citation statements)

References 19 publications

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“…ICP, duration of tumescence (DT) and SAP were recorded after the intracavernosal administration of cumulative doses of chuanxiongzine and the same volume of normal saline. To minimize the effect of the previous drug, we washed the cavernous body with 0.15 ml normal saline before each injection and the interval between the two injections was at least 1 h. 29,30 Data analysis All data were expressed as mean±s.e.m. (standard error of mean).…”

Section: Drugs and Solutionsmentioning

confidence: 99%

Chuanxiongzine relaxes isolated corpus cavernosum strips and raises intracavernous pressure in rabbits

et al. 2009

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It has been shown that there are many Chinese traditional herbals that can enhance sexual activity. Chuanxiongzine is a vasoactive ingredient that has been isolated and purified from Ligusticum chuanxiong Hort. In previous studies, it has been found that chuanxiongzine was effective in relaxing rabbit corpus cavernosum smooth muscle. We determined the effects of chuanxiongzine on relaxation of isolated corpus cavernosum strips in vitro and on increase of intracavernous pressure (ICP) in vivo in rabbits. Chuanxiongzine caused a concentration-dependent relaxation of phenylephrine precontracted isolated corpus cavernosum strips (EC 50 1.58 Â 10 À4 mol l À1 ), which were endothelium independent and NO independent. However, the guanylyl cyclase inhibitor 1-H-[1,2,4] oxadiazolo [4,3-a] quinoxalin-1-one significantly shifted the chuanxiongzine concentration-response relationship to the right. Although there was no significant difference in the level of cyclic guanosine monophosphate (cGMP) and cyclic adenosine monophosphate (cAMP) in isolated corpus cavernosum strips treated with chuanxiongzine or vehicle, chuanxiongzine caused a significant rise in the level of cGMP and cAMP in isolated corpus cavernosum strips pretreated with the activator of adenylyl cyclase forskolin and the source of NO sodium nitroprusside. In an in vivo study, chuanxiongzine dose-dependently raised ICP after the intracavernous injection of its cumulative doses (0.5, 1, 2 and 5 mg kg À1 ). The ICP increased from baseline to 19.1±3.7, 24.8±2.1, 30.2 ± 4.8 and 39.7 ± 6.1 mm Hg, respectively, and the duration of tumescence ranged from 8.5 ± 2.8 to 22.9 ± 7.3 min. Our results show that chuanxiongzine can relax isolated corpus cavernosum strips of rabbits in vitro and increase ICP of rabbits in vivo, which is neither endothelium dependent nor NO dependent, but may be partly mediated by the inhibition of cAMP phosphodiesterase or cGMP phosphodiesterase.

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Section: Drugs and Solutionsmentioning

confidence: 99%

Chuanxiongzine relaxes isolated corpus cavernosum strips and raises intracavernous pressure in rabbits

et al. 2009

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“…These structures also give required rigidity to the penis when in a flaccid state (2,3). Several studies have reported functional and morphological changes that take place during the erection process in different animal models (4,5) and also in humans (6,7).…”

Section: Introductionmentioning

confidence: 99%

“…Both CC and CS are covered by a dense connective layer, the tunica albuginea, which originates intra-cavernosum pillars or septa, mainly in the CC (14). There are pronounced resemblances between the rabbit and human penis in morphological, physiological, and neurological features (5,15). Therefore, the rabbit penis is often used as an experimental model to assess ED (5,14).…”

Section: Introductionmentioning

confidence: 99%

“…There are pronounced resemblances between the rabbit and human penis in morphological, physiological, and neurological features (5,15). Therefore, the rabbit penis is often used as an experimental model to assess ED (5,14). The rabbit penis is a more suitable model than the rat penis, which is classified as a fibro-elastic type (4), with a penile bone inside and a penile protrusion during mating with little variation in its diameter (16).…”

Section: Introductionmentioning

confidence: 99%

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Sinusoidal Constriction and Vascular Hypertrophy in the Diabetes-Induced Rabbit Penis

et al. 2013

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Objective: To assess the morphological changes of penile vascular structures and the corpus cavernosum area in alloxan-induced diabetic rabbits. Materials and Methods: Twenty male rabbits (2 months old) were divided into two groups with 10 rabbits each, the control group (CG) and the diabetic group (DG). The animals from DG received an intravenous injection of alloxan (100mg/kg) to induce the diabetes. Ten weeks after the induction of diabetes, all animals were euthanized. Two fragments of the penile shaft were harvested and samples were processed and paraffin embedded. Sections (5µm) were cut and stained for histological and immunohistochemical markers. Results: Nuclear protrusion toward the lumen, and cytoplasmic vacuolization were observed in the tunica intima of the dorsal artery of the penis in DG. The thicknesses of the tunica media increased significantly in DG (p = 0.0350). It was also observed a significant increase in the area of the tunica media (p = 0.0179). There was no significant change in smooth muscle cell density in the tunica media of the dorsal artery of the penis (p = 0.0855). The collagen fiber pattern of the tunica adventitia of the dorsal artery of the penis was different between the control and diabetic groups. There was a significant decrease in the area occupied by the cavernous sinuses in DG (p = 0.0013). Conclusion: Alloxan-induced diabetes mellitus in rabbits promotes important changes in penile vascular structures, thereby decreasing blood supply and affecting penile hemodynamics, leading to erectile dysfunction.

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“…The general understanding of the morphological changes and physiology of penile erection is obtained through several studies considering different animal models [1][2][3] such as rats, 1,4,5 domestic animals, primates 3,[6][7][8] and rabbits. 2,9 Morphological and quantitative data concerning rabbit penis are still scarce, and there is need for more information, mainly because this animal is frequently used as a model for erectile dysfunction.…”

Section: Introductionmentioning

confidence: 99%

Concentration of elastic system fibers in the corpus cavernosum, corpus spongiosum, and tunica albuginea in the rabbit penis

et al. 2005

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The corpus cavernosum (CC) extracellular matrix is essential for normal penile erection and is implicated in erectile dysfunction. Although investigations of these issues have used the rabbit CC, organization of its components is not well known to date. We characterized and quantified the volumetric density (Vv) of the elastic system fibers in the corpus spongiosum (CS), CC and tunica albuginea (TA) of the rabbit penis. Adult New Zealand rabbits (n ¼ 10) were used. The penile midshaft fragments were fixed with 4% phosphate-buffered formalin solution and/or Bouin's liquid for 24-48 h, and processed using standard histological techniques. The sections were stained with Weigert's Fucsin-Resorcin with previous oxidation. The elastic system fibers Vv (%) was determined in 25 random fields of each fragment, using the M-42 test grid. The histochemical methods detected elastic system fibers in CS, CC and TA of all animals. The Vv of elastic fibers average was 25.0372.0% for CC, 32.2371.41% for CS and 22.3873.61% for TA. Results for CC and CS were not significantly different. The great amount of elastic fibers distribution beneath the endothelium suggest that these fibers may have an important role in the erection process in rabbits. The present data should therefore provide important information for devising experiments and interpreting results when using the rabbit penis as a model for penile dysfunctions, especially when making comparisons with humans.

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Rabbits as models for impotence research

Abstract: AimThe aim of this presentation is to answer the question`Why are rabbits useful models for impotence research?'

Cited by 13 publications

References 19 publications

Chuanxiongzine relaxes isolated corpus cavernosum strips and raises intracavernous pressure in rabbits

Chuanxiongzine relaxes isolated corpus cavernosum strips and raises intracavernous pressure in rabbits

Sinusoidal Constriction and Vascular Hypertrophy in the Diabetes-Induced Rabbit Penis

Concentration of elastic system fibers in the corpus cavernosum, corpus spongiosum, and tunica albuginea in the rabbit penis

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