Rabbit Sera Containing Compound Danshen Dripping Pill Attenuate Leukocytes Adhesion to TNF-Alpha–Activated Human Umbilical Vein Endothelial Cells by Suppressing Endothelial ICAM-1 and VCAM-1 Expression Through NF-KappaB Signaling Pathway

Abstract

The adherence to circulating leukocytes, such as monocytes and neutrophils, to vascular endothelial cells is of central importance to the pathogenesis of various cardiovascular diseases (CVDs) including atherosclerosis and myocardial ischemia-reperfusion injury. Compound danshen dripping pill (CDDP; Fufang Danshen Diwan in Chinese), namely cardiotonic pill, is extensively used for CVDs medication in China and some other countries. Here, we sought to investigate the effect of CDDP on leukocytes binding to vascular endothelial cells and elaborate the possibly underlying mechanism. Using seropharmacological method, rabbit sera containing CDDP were shown to mitigate the adhesiveness of monocytes and neutrophils to tumor necrosis factor alpha-stimulated human umbilical vein endothelial cells in dose and time-dependent manners, alleviate the levels of intercellular adhesion molecule 1 and vascular cell adhesion molecule 1 messenger RNA and protein dose dependently and also encumber IκBα degradation, p65 nuclear translocation, nuclear factor-kappaB (NF-κB) DNA-binding activity, and NF-κB-responsive gene transcription in tumor necrosis factor alpha-activated human umbilical vein endothelial cells. This study suggests that CDDP protects against CVDs potentially by attenuation of leukocytes-endothelium adhesion cascade via lessening endothelial cell adhesion molecules expression and NF-κB signaling pathway activity.

Ischemic preconditioning ameliorates intestinal injury induced by ischemia-reperfusion in rats

“…Accumulating evidence demonstrates that leukocyte-endothelial adhesion is mediated by cell adhesion molecules on vascular endothelial cells and counterpart ligands on leukocytes, especially in IR [30,31] . ICAM-1 and VCAM-1 expressed on these cells are central for the adherence of neutrophils and monocytes to the endothelium [32] .…”

Compound Bieshe Kang’ai inhibits proliferation and induces apoptosis in HCT116 human colorectal cancer cells

“…Mounting evidence reveals that traditional Chinese medicines have some advantages, such as acting on multiple pathological targets and possessing reduced adverse reactions, over modern chemotherapeutic agent for many types of diseases [3,4], particularly involving cancers [5]. Here, we present a Chinese medicinal formula CBK that is efficacious to varieties of cancers in clinical practice, and the underlying mechanism to be delineated.…”

Effect of total peony glucoside pretreatment on NF-κB and ICAM-1 expression in myocardial tissue of rat with myocardial ischemia-reperfusion injury

“…Generally, NF-κB is inactive in the cytoplasm. Once activated, NF-κB promotes inhibitor of kappa B (IκB)-α expression, which in return regulates NF-κB expression (Boyd et al, 2006;Xu et al, 2014). IL-1 and lipopolysaccharide can cause IκB degradation, leading to NF-κB activation.…”

“…IκB phosphorylation and decomposition, along with NF-κB translocation may relate to acute inflammation-induced injury (Frantz et al, 2007;Xu et al, 2014). NF-κB can bind to various upstream promoters and enhancers of the genes coding ICAM-1 and IL-8, and upregulate their gene expression (Gu et al, 2006;Xu et al, 2014). ICAM-1 is a ligand of the integrin lymphocyte function-associated antigen-1, which promotes endothelial cell and leukocyte adhesion.…”