Ischemic preconditioning ameliorates intestinal injury induced by ischemia-reperfusion in rats

Ji, Yuanyuan; Wang, Zhidong; Wang, Shufeng; Wang, Bao-tai; Yang, Zhaohui; Zhou, Xiaorong; Lei, Ni-Na; Yue, Wei-Na

doi:10.3748/wjg.v21.i26.8081

Cited by 29 publications

(32 citation statements)

References 34 publications

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“…Recently, Ji and associates showed that intestinal IPC significantly decreased intestinal myeloperoxidase levels. 5 Our study is consistent with published data in which antithrombin decreased myeloperoxidase activity; in addition, it was proven synergistic with IPC. The fact that the myeloperoxidase levels in the AT+IPC group were not significantly higher than in the sham group stresses the decisive role of antithrombin in dampening the IRI.…”

Section: Discussionsupporting

confidence: 92%

“…30 Yin and associates and Ji and associates have shown that IPC prevents TNF-α elevation after IRI. 5,31 Our study concurs with published data and shows that TNF-α levels after 30 minutes of ischemia and 60 minutes of reperfusion were lower in animals pretreated with IPC. Levels of TNF-α were decreased by brief IPC, and this confirms the role of TNF-α as one of the mediators responsible for the remote effects of IPC.…”

Section: Discussionsupporting

confidence: 92%

“…5 The groups that underwent IPC have significantly reduced TNF-α and IL-6 in the blood. In addition, myeloperoxidase as an index of polymorphonuclear accumulation was significantly less in the IPC groups and especially in the groups treated with AT, in agreement with similar studies.…”

Section: Discussionmentioning

confidence: 94%

“…Initiators of this pathop hysiology are regulated by the transcript factor nuclear factor-κB. 4,5 Various methods with protective effects have been tested to ameliorate IRI. Conditioning (preconditioning, postconditioning, or remote conditioning) is among the therapeutic approaches used to enhance organ ischemia tolerance.…”

Section: Introductionmentioning

confidence: 99%

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Vrakas

Tsalis²,

Roidos³

et al. 2017

Exp Clin Transplant

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Objectives: Our study aimed to determine whether antithrombin plays a synergistic role in accentuating the effects of intestinal ischemic preconditioning. Materials and Methods: Fifty rats were randomly allocated to 5 groups (10 rats/group) as follows: sham treatment (group 1); ischemia-reperfusion (group 2); ischemic preconditioning followed by ischemiareperfusion (group 3); antithrombin + ischemia-reperfusion, similar to group 2 but including antithrombin administration (group 4); and antithrombin + ischemic preconditioning, similar to group 3 but including antithrombin administration (group 5). Blood samples and liver specimens were obtained for measurement of cytokines, myeloperoxidase, and malondialdehyde. Liver biopsies were examined by electron microscopy. Results: Intestinal ischemia-reperfusion induced a remote hepatic inflammatory response as evidenced by the striking increase of proinflammatory cytokines, myeloperoxidase, and malondialdehyde. Tumor necro sis factor-α levels in group 5 (12.48 ± 0.7 pg/mL) were significantly lower than in group 3 (13.64 ± 0.78 pg/mL; P = .014). Mean interleukin 1β was lower in group 5 (9.52 ± 0.67pg/mL) than in group 3 (11.05 ± 1.9 pg/mL; P > .99). Mean interleukin 6 was also significantly lower in group 5 (17.13 ± 0.54 pg/mL) than in group 3 (23.82 ± 1 pg/mL; P < .001). Myeloperoxidase levels were significantly higher in group 3 (20.52 ± 2.26 U/g) than in group 5 (18.59 ± 1.03 U/g; P = .025). However, malondialdehyde levels did not significantly improve in group 5 (4.55 ± 0.46 μmol) versus group 3 (5.17 ± 0.61 μmol; P = .286). Tumor necrosis factor-α, interleukin 6, and myeloperoxidase findings show that antithrombin administration further attenuated the inflammatory response caused by ischemia-reperfusion, suggesting a synergistic effect with ischemic preconditioning. These findings were confirmed by electron microscopy. Conclusions: The addition of antithrombin to ischemic preconditioning may act to attenuate or prevent damage from ischemia-reperfusion injury by inhi biting the release of cytokines and neutrophil infiltration.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Untitled

Vrakas

Tsalis²,

Roidos³

et al. 2017

Exp Clin Transplant

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“…Therefore, the oxidative activity should be proportional with the activity and number of these cells. MPO level in a tissue is directly proportional with the number of activated neutrophils and Kupffer cells that was previously used to evaluate the I/R injury in both liver and small intestine 18,25 . .…”

Section: Results Of Histopathological Analysismentioning

confidence: 99%

Grape seed protects cholestatic rats liver from ischemia/reperfusion injury

et al. 2016

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PURPOSE:To determine the effect of grape-seed extract against ischemia/reperfusion injury in cholestatic liver. METHODS:Eighteen Wistar albino rats were divided into three groups. In control and study groups, cholestasis was provided by bile duct ligation. Seven days later, the rats were subjected to 30 min hepatic ischemia, followed by 60 min of reperfusion. Oral administration of 50 mg/kg/day grape-seed extract was started 15 days before bile duct ligation and continued to the second operation in the study group. Serum, plasma and liver samples were taken. Laboratory analysis, tissue gluthation, malondialdehyde, myeloperoxidase levels and histopathological examination were performed.RESULTS: Significant decrease in liver gluthation level and significant increase in malondialdehyde level and myeloperoxidase activity were observed after ischemia/reperfusion in cholestatic rats. Serum and plasma levels for laboratory analysis were also significantly higher in cholestatic I/R group. Hepatic necrosis and fibrosis were detected in histopathological examination. Oral grape-seed extract administiration reversed all these parameters and histopathological findings except serum bilirubin levels. CONCLUSION:Oral grape-seed extract treatment can improve liver functions and attenuate the inflammation and oxidative stress in cholestatic ischemia/reperfusion injury.

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Ischaemic Preconditioning: The Rationale and Evidence-Based Outcomes

Hamilton

2019

Visceral Vessels and Aortic Repair

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Ischemic preconditioning ameliorates intestinal injury induced by ischemia-reperfusion in rats

Cited by 29 publications

References 34 publications

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Grape seed protects cholestatic rats liver from ischemia/reperfusion injury

Ischaemic Preconditioning: The Rationale and Evidence-Based Outcomes

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