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Vettore, André Luiz; Yunes, José Andrés; Neto, Germano Cord; Silva, Márcio José da; Arruda, Paulo; Leite, Adílson

doi:10.1023/a:1005995806897

Cited by 42 publications

(11 citation statements)

References 52 publications

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“…5B, high levels of GUS activity were detected in aleurone and subaleurone layers of maturing seeds and not in the embryo tissues. Highly sensitive fluorometric assays also showed that GUS activity was not (19) were from barley, O2 (15) and OHP1 (34) from maize, SPA (5) from wheat, O2-sorg from sorghum (38), and O2-coix from coixin (39). detected in roots, leaf, stem, and anthers (data not shown).…”

Section: Isolation Of Cdna Clones Encoding Bzip Transcription Factor mentioning

confidence: 95%

A Rice Functional Transcriptional Activator, RISBZ1, Responsible for Endosperm-specific Expression of Storage Protein Genes through GCN4 Motif

Onodera¹,

Suzuki²,

Wu³

et al. 2001

Journal of Biological Chemistry

152

149

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The GCN4 motif, a cis-element that is highly conserved in the promoters of cereal seed storage protein genes, plays a central role in controlling endospermspecific expression. This motif is the recognition site for a basic leucine zipper transcriptional factor that belongs to the group of maize Opaque-2 (O2)-like proteins. Five different basic leucine zipper cDNA clones, designated RISBZ1-5, have been isolated from a rice seed cDNA library. The predicted gene products can be divided into two groups based on their amino acid sequences. Although all the RISBZ proteins are able to interact with the GCN4 motif, only RISBZ1 is capable of activating (more than 100-fold expression) the expression of a reporter gene under a minimal promoter fused to a pentamer of the GCN4 motif. Loss-of-function and gain-of-function experiments using the yeast GAL4 DNA binding domain revealed that the proline-rich N-terminal domain (27 amino acids in length) is responsible for transactivation. The RISBZ1 protein is capable of forming homodimers as well as heterodimers with other RISBZ subunit proteins. RISBZ1 gene expression is restricted to the seed, where it precedes the expression of storage protein genes. When the RISBZ1 promoter was transcriptionally fused to the ␤-glucuronidase reporter gene and the chimeric gene was introduced into rice, the ␤-glucuronidase gene is specifically expressed in aleurone and subaleurone layer of the developing endosperm. These findings suggest that the specific expression of transcriptional activator RISBZ1 gene may determine the endosperm specificity of the storage protein genes.Regulated gene expression is mediated by the combinatorial interactions of multiple cis-elements in the gene's promoter. Specific binding of transcriptional factors to the cognate ciselements constitute a crucial step in transcription initiation and, in turn, on the spatial and temporal expression of genes.Seed storage protein genes provide a model system for the study on the regulatory mechanisms of plant genes (1), since their expression is restricted to a specific tissue and stage during seed development. These specific temporal and spatial expression patterns may be explained as the result of regulatory assemblies of several transcriptional activators that recognize the cis-elements implicated in seed-specific expression. Therefore, to understand such molecular mechanisms, characterization of cis-elements and transcription factors has been performed on many storage protein genes of several crop plants (2,3). Despite numerous studies, the mechanism by which these genes are regulated are poorly understood, since many of the essential cis-elements have not been identified. This is especially true in the case of monocot plants, where many of the promoter analyses of cereal storage protein genes have carried out by transient assays using particle bombardment or heterologous transgenic tobacco system (4 -6). Dissection analyses of promoter using homologous stable transgenic plant have been carried out only on glutelin genes of ...

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Section: Isolation Of Cdna Clones Encoding Bzip Transcription Factor mentioning

confidence: 95%

A Rice Functional Transcriptional Activator, RISBZ1, Responsible for Endosperm-specific Expression of Storage Protein Genes through GCN4 Motif

Onodera¹,

Suzuki²,

Wu³

et al. 2001

Journal of Biological Chemistry

152

149

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“…Garcia and Villarroel (2009) found that corn gluten had a positive influence on macrophage phagocytosis in tilapia (Oreochromis niloticus L.), and the number of bacteria that was phagocytosed by macrophages was significantly increased at 4-h postinfection. The nucleotide and amino acid sequence of Coix exhibited high homology with wheat and corn (Ary, Shewry, & Richardson, 1988;Vettore et al, 1998). Thus, the function of CGH on immune system is worth to be investigated.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory activity of small molecular (≤3 kDa)Coixglutelin enzymatic hydrolysate

et al. 2016

CyTA - Journal of Food

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2017)Immunomodulatory activity of small molecular (≤3 kDa) Coix glutelin enzymatic hydrolysate, CyTA -Journal of Food, 15:1, 41-48, ABSTRACT In this study, small molecular (≤3 kDa) Coix glutelin hydrolysate (CGH) was obtained by the method of enzymatic hydrolysis and then immunomodulatory activity was evaluated in vitro and in vivo. Stimulation index of mice splenocytes was increased in a dose-dependent manner after treated with 3.125-25 μg/mL CGH. Acid phosphatase in mice peritoneal macrophages was significantly improved after stimulated with CGH at some concentration. The CGH stimulated the secretion of NO from Raw264.7 in a concentration-dependent manner and also significantly inhibited lipopolysaccharideinduced cell excessive activation at some extent. After successive administration of CGH for 21 days, the spleen index of ICR was significantly increased to 3.73 mg/g for the group administrated 800 mg/kg CGH. Malondialdehyde (MDA) assay displayed that 200 mg/kg CGH increased mice serum MDA concentration, while the variation was obsolete in the other CGH groups. All results demonstrated that CGH took essential effects on immunomodulatory activities.Actividad inmunomoduladora de hidrolisato glutelina encimático de moléculas pequeñas de Coix (≤ 3 kDa) RESUMEN En este estudio, se obtuvo hidrolisato glutelina de Coix (CGH) de molécula pequeña (≤ 3 kDa) mediante el método de hidrólisis enzimática y después se evaluó la actividad sinmunomoduladora in vitro y in vivo. El índice de estimulación (SI) de los esplenocitos de ratón aumentó de forma dependiente a la dosis después ser tratados con 3,125-25 μg/mL de CGH. La fosfatasa ácida (ACP) en los macrófagos peritoneales de ratón mejoró significativamente después de que se estimulara con CGH en algunas concentraciones. El CGH estimuló la secreción de NO a partir de Raw264.7 de forma dependiente de la concentración e inhibió de forma significativa la activación excesiva de células inducidas con LPS en cierta medida. Después de una administración sucesiva de CGH durante 21 días, el índice esplénico de ICR aumentó significativamente a 3,73 mg/g para el grupo al cual se había administrado 800 mg/kg de CGH. El ensayo de malondialdehído (MDA) mostró que los 200 mg/kg de CGH hicieron aumentar la concentración de MDA del suero de ratón, mientras que la variación fue obsoleta en los otros grupos de CGH. Todos los resultados demostraron que CGH obtuvo efectos esenciales en las actividades inmunomoduladoras. ARTICLE HISTORY

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“…Genetic, molecular and biochemical analyses indicate that bZIPs are regulators of important plant processes such as organ and tissue differentiation [26]–[30], cell elongation [31], [32], nitrogen/carbon balance control [33], [34], pathogen defence [35]–[40], energy metabolism [41], unfolded protein response [42], [43], hormone and sugar signalling [44]–[47], light response [48]–[50], osmotic control [34], [51], and seed storage protein gene regulation [52]. Initially, 50 plant bZIP proteins were classified into five families, taking into account similarities of their bZIP domain [53]. An original investigation of the complete Arabidopsis thaliana genome sequence indicated the presence of 81 putative bZIP genes [54], [55].…”

Section: Introductionmentioning

confidence: 99%

The Role of bZIP Transcription Factors in Green Plant Evolution: Adaptive Features Emerging from Four Founder Genes

et al. 2008

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BackgroundTranscription factors of the basic leucine zipper (bZIP) family control important processes in all eukaryotes. In plants, bZIPs are regulators of many central developmental and physiological processes including photomorphogenesis, leaf and seed formation, energy homeostasis, and abiotic and biotic stress responses. Here we performed a comprehensive phylogenetic analysis of bZIP genes from algae, mosses, ferns, gymnosperms and angiosperms.Methodology/Principal FindingsWe identified 13 groups of bZIP homologues in angiosperms, three more than known before, that represent 34 Possible Groups of Orthologues (PoGOs). The 34 PoGOs may correspond to the complete set of ancestral angiosperm bZIP genes that participated in the diversification of flowering plants. Homologous genes dedicated to seed-related processes and ABA-mediated stress responses originated in the common ancestor of seed plants, and three groups of homologues emerged in the angiosperm lineage, of which one group plays a role in optimizing the use of energy.Conclusions/SignificanceOur data suggest that the ancestor of green plants possessed four bZIP genes functionally involved in oxidative stress and unfolded protein responses that are bZIP-mediated processes in all eukaryotes, but also in light-dependent regulations. The four founder genes amplified and diverged significantly, generating traits that benefited the colonization of new environments.

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Cited by 42 publications

References 52 publications

A Rice Functional Transcriptional Activator, RISBZ1, Responsible for Endosperm-specific Expression of Storage Protein Genes through GCN4 Motif

A Rice Functional Transcriptional Activator, RISBZ1, Responsible for Endosperm-specific Expression of Storage Protein Genes through GCN4 Motif

Immunomodulatory activity of small molecular (≤3 kDa)Coixglutelin enzymatic hydrolysate

The Role of bZIP Transcription Factors in Green Plant Evolution: Adaptive Features Emerging from Four Founder Genes

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