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Kulczykowska, Ewa

doi:10.1023/a:1021348822635

Cited by 35 publications

(3 citation statements)

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“…Salinity adaptation in euryhaline teleosts is a complex process involving physiological responses in several osmoregulatory organs. In fish, the kidney is an important osmoregulatory organ involved in maintaining osmotic homeostasis [13, 45]. Functional changes are reflected by the differential expression of various genes in the kidney.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of renal dopamine-mediated Na+ homeostasis response to environmental salinity stress in Scatophagus argus

Zhou

Duan

et al. 2019

BMC Genomics

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Background To control the osmotic pressure in the body, physiological adjustments to salinity fluctuations require the fish to regulate body fluid homeostasis in relation to environmental change via osmoregulation. Previous studies related to osmoregulation were focused primarily on the gill; however, little is known about another organ involved in osmoregulation, the kidney. The salinity adaptation of marine fish involves complex physiological traits, metabolic pathways and molecular and gene networks in osmoregulatory organs. To further explore of the salinity adaptation of marine fish with regard to the role of the kidney, the euryhaline fish Scatophagus argus was employed in the present study. Renal expression profiles of S. argus at different salinity levels were characterized using RNA-sequencing, and an integrated approach of combining molecular tools with physiological and biochemical techniques was utilized to reveal renal osmoregulatory mechanisms in vivo and in vitro. Results S. argus renal transcriptomes from the hyposaline stress (0‰, freshwater [FW]), hypersaline stress (50‰, hypersaline water [HW]) and control groups (25‰) were compared to elucidate potential osmoregulatory mechanisms. In total, 19,012 and 36,253 differentially expressed genes (DEGs) were obtained from the FW and HW groups, respectively. Based on the functional classification of DEGs, the renal dopamine system-induced Na + transport was demonstrated to play a fundamental role in osmoregulation. In addition, for the first time in fish, many candidate genes associated with the dopamine system were identified. Furthermore, changes in environmental salinity affected renal dopamine release/reuptake by regulating the expression of genes related to dopamine reuptake ( dat and nkaα1 ), vesicular traffic-mediated dopamine release ( pink1 , lrrk2 , ace and apn ), DAT phosphorylation ( CaMKIIα and pkcβ ) and internalization ( akt1 ). The associated transcriptional regulation ensured appropriate extracellular dopamine abundance in the S. argus kidney, and fluctuations in extracellular dopamine produced a direct influence on Na + /K + -ATPase (NKA) expression and activity, which is associated with Na + homeostasis. Conclusions These transcriptomic data provided insight into the molecular basis of renal osmoregulation in S. argus . Significantly, the results of this study revealed the mechanism of renal dopamine system-induced Na + transport is essential in fish osmoregulation. ...

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Section: Discussionmentioning

confidence: 99%

Transcriptional analysis of renal dopamine-mediated Na+ homeostasis response to environmental salinity stress in Scatophagus argus

Zhou

Duan

et al. 2019

BMC Genomics

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“…As a potent regulator of circadian and seasonal rhythms, the pineal neurohormone melatonin is involved in several physiological activities in vertebrates including fishes ( Velarde et al, 2010 ; Esteban et al, 2013 ; Mukherjee et al, 2014 ; Mukherjee and Maitra, 2015 ; Muñoz-Pérez et al, 2016 ; Sánchez-Vázquez et al, 2019 ). This multifunctional hormone has a control on the regulation of ionosmotic homeostasis in fishes ( Kulczykowska, 2002 ; Kulczykowska et al, 2006 ; Falcon et al, 2007 ; Sangiao-Alvarellos et al, 2007 ; Ngasainao and Lukram, 2016 ). For example, melatonin is known to influence water and mineral balance in mammals ( Tsuda et al, 1995 ; Drew et al, 2001 ; SajeebKhan and Peter, 2015 ), birds ( Ching et al, 1999 ) and amphibians ( Wright et al, 1997 ).…”

Section: Introductionmentioning

confidence: 99%

“…For example, melatonin is known to influence water and mineral balance in mammals ( Tsuda et al, 1995 ; Drew et al, 2001 ; SajeebKhan and Peter, 2015 ), birds ( Ching et al, 1999 ) and amphibians ( Wright et al, 1997 ). The widespread distribution of melatonin receptors in the osmoregulatory tissues of both freshwater and seawater fishes indicates that these tissues are the possible sites for melatonin action ( Kulczykowska, 2002 ; Kulczykowska et al, 2006 ). Moreover, studies have reported that salinity can modify the melatonin contents in fish intestine and gills along with its levels in plasma ( Lopez-Olmeda et al, 2009 ), pointing to a role for extra-pineal source in melatonin homeostasis in fish.…”

Section: Introductionmentioning

confidence: 99%

Melatonin integrates multidimensional regulation of Na+/K+-ATPase in ionocytes and promotes stress and ease response in hypoxia-induced air-breathing fish: lessons from integrative approach

et al. 2023

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As circadian regulator, melatonin is involved in many physiological processes including ionosmotic regulation in fishes. Na+/K+-ATPase (NKA), an ubiquitous Na+/K+ transporter in ionocyte epithelia that drives electrochemical Na+ gradients and systemic osmotic integration, is a target of stress in fish. However, it is not certain how melatonin regulates NKA functions in ionocyte epithelia and how it modulates the adaptive response such as stress and ease response in fish particularly in hypoxia condition. We, thus, examined the short-term in vivo action of melatonin on the dynamics of NKA regulation in branchial, renal and intestinal ionocytes of hypoxia-induced air-breathing fish (Anabas testudineus Bloch). Interestingly, we found a rise in plasma melatonin in fish when kept for 30 min of forced submergence in water and that indicates a role for melatonin in hypoxia tolerance. A fall in blood [Na+, K+] occurred in these hypoxic fish which later showed a recovery after melatonin treatment. Similarly, melatonin favored the fall in NKA activity in branchial and renal epithelia of hypoxic fish, though it remarkably stimulated its activities in non-stressed fish. Likewise, melatonin that produced differential pattern of mRNA expression in nkaα1-subunit isoforms (nkaα1a, nkaα1b and nkaα1c) and melatonin receptor isoforms (mtnr1a, mtnr1bb, mtnr1bbx1x2) in the tested ionocyte epithelia, showed reversed expression in hypoxic fish. In addition, the rise in NKAα-protein abundance in branchial and renal epithelia of melatonin-treated hypoxic fish indicated a recovery action of melatonin. A higher NKAα-immunoreactivity was found in the immunohistochemical and immunofluorescent images of branchial ionocytes and renal proximal and distal ionocytes of hypoxic fish treated with melatonin. Furthermore, an activation of PKA and PKG-dependent phosphorylation was found in branchial epithelia of hypoxic fish. The generated integrative parabola model showed that melatonin has a maximum targeted action on NKA function in the renal epithelia, suggesting its lead role in the integration of ionosmotic balance during the recovery or ease response. Over all, the data indicate a multidimensional and preferential action of melatonin on NKA regulation in fish ionocytes that integrate the recovery action against hypoxia, thus pointing to a major role for melatonin in stress and ease response in this fish.

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Day–night specific binding of 2-[125I]Iodomelatonin and melatonin content in gill, small intestine and kidney of three fish species

et al. 2005

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Some of melatonin's (Mel) well-established physiological effects are mediated via high-affinity cell-membrane receptors belonging to the superfamily of G-protein-coupled receptors. Specific binding of ligand 2-[(125)I]iodomelatonin, using membrane preparations from osmoregulatory tissues of flounder, rainbow trout and sea bream, together with Mel concentrations in the tissues and plasma were studied. The kidney, gill and small intestine samples were collected during the day and at night. The dissociation constants (K (d)) and maximal binding densities (B (max)) were calculated for each tissue at 11:00 and 23:00 h. The binding sites with K (d) values in the tissues in the picomolar range indicated the high affinity. K (d) and B (max) values were tissue- and species-dependent. The GTP analogue [Guanosine 5'-O-(3-thiotriphosphate)] treatment significantly reduced the B (max) value, indicating that the 2-[(125)I]iodomelatonin-binding sites are probably coupled to a G-protein. No daily variations in K (d) and B (max) values were observed. These are the first studies of the presence of 2-[(125)I]iodomelatonin-binding sites in the small intestine, kidney tubule and gill of fish. The data strongly suggest new potential targets for Mel action and the influence of Mel on water/ion balance in fish. The intestine seems to be a site of Mel synthesis and/or an active accumulation of the hormone.

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Cited by 35 publications

References 70 publications

Transcriptional analysis of renal dopamine-mediated Na+ homeostasis response to environmental salinity stress in Scatophagus argus

Transcriptional analysis of renal dopamine-mediated Na+ homeostasis response to environmental salinity stress in Scatophagus argus

Melatonin integrates multidimensional regulation of Na+/K+-ATPase in ionocytes and promotes stress and ease response in hypoxia-induced air-breathing fish: lessons from integrative approach

Day–night specific binding of 2-[125I]Iodomelatonin and melatonin content in gill, small intestine and kidney of three fish species

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