(R)evolution of an Idea

McEvoy-Levy, Siobhán

doi:10.1057/9780333977835_2

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…Lewis rat islets and RIN cells, but not to GH3 cells, have been described in diabetic BB rats (26). In addi tion, cytotoxic cell-mediated immunoreactivity selec tively against a P-cell line was also observed in the mul tiple low-dose streptozotocin-induced rodent model of autoimmune diabetes (27).…”

Section: Introductionmentioning

confidence: 97%

Insulin-Secreting Pituitary GH3 Cells: A Potential β-Cell Surrogate for Diabetes Cell Therapy

et al. 2000

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In a companion article, we describe the engineering and characterization of pituitary GH3 cell clones stably transfected with a furin-cleavable human insulin cDNA (InsGH3 cells). This article describes the performance of InsGH3 (clones 1 and 7) cell grafts into streptozotocin (STZ)-induced diabetic nude mice. Subcutaneous implantation of 2 x 10(6) InsGH3 cells resulted in the progressive reversal of hyperglycemia and diabetic symptoms, even though the progressive growth of the transplanted cells (clone 7) eventually led to glycemic levels below the normal mouse range. Proinsulin transgene expression was maintained in harvested InsGH3 grafts that, conversely, lose the expression of the prolactin (PRL) gene. Elevated concentrations of circulating mature human insulin were detected in graft recipients, demonstrating that proinsulin processing by InsGH3 cells did occur in vivo. Histologic analysis showed that transplanted InsGH3 grew in forms of encapsulated tumors composed of cells with small cytoplasms weakly stained for the presence of insulin. Conversely, intense insulin immunoreactivity was detected in graft-draining venules. Compared to pancreatic betaTC3 cells, InsGH3 cells showed in vitro a higher rate of replication, an elevate resistance to apoptosis induced by serum deprivation and proinflammatory cytokines, and significantly higher antiapoptotic Bcl-2 protein levels. Moreover, InsGH3 cells were resistant to the streptozotocin toxicity that, in contrast, reduced betaTC3 cell viability to 50-60% of controls. In conclusion, proinsulin gene expression and mature insulin secretion persisted in transplanted InsGH3 cells that reversed hyperglycemia in vivo. InsGH3 cells might represent a potential beta-cell surrogate because they are more resistant than pancreatic beta cells to different apoptotic insults and might therefore be particularly suitable for encapsulation.

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Section: Introductionmentioning

confidence: 97%

Insulin-Secreting Pituitary GH3 Cells: A Potential β-Cell Surrogate for Diabetes Cell Therapy

et al. 2000

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“…Along with this phenomenon, many non-permanent grasslands are established for various purposes, generally for animal feed (grazing area). Grazed pastures may sequester more C than grasslands used for silage or hayproduction, due to the recycling of organic matter and nutrients from faeces and plant residues [12]. However, continuous heavy grazing would be detrimental to the growth of the herbaceous plants which in turn may lead to the depletion of aboveground and soil carbon stocks [13].…”

Section: Introductionmentioning

confidence: 99%

Carbon Sequestration Potentials of Man-made Grasslands under Different Pattern of Plantation Stands in West Bandung Regency, Indonesia

et al. 2021

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Alternatives of vegetations to store carbon need to be encouraged considering that forests are threatened by widespread destructions. One such vegetation is grasslands which have the potential for carbon storage and to reduce CO2 concentration in the atmosphere. At present, many enterprises have designed grasslands for many purposes. Grasslands at Cikalong Wetan and Little Farmers, West Bandung Regency were established under different pattern of plantation stands. The purpose of this research was to study the potential of carbon stock in grassland vegetation at these locations. Based on RaCSA method, the tree biomass was determined by nondestructive collection of density and basal area of trees, then calculated by an allometric equation. The ground cover biomass was determined by destructive collection of grass and roots. Total measured biomass was multiplied by 46% to obtain carbon storage. Based on the results, the potential of carbon stock in Little Farmers grassland (159,540 ton ha-1) is higher than in Cikalong Wetan (14,482 ton ha-1). Carbon stored in tree biomass gives the highest contribution to overall carbon stock potential in Little Farmers (94.84%) while carbon stored in below-ground understorey biomass gives the highest contribution in Cikalong Wetan (52.13 %). Different management of grasslands and pattern of plantation stands resulting a different contribution of carbon stock in every carbon pool. In order to maintain the carbon sequestration potentials of these locations, an agroforestry management such as agri-silviculture need to be encouraged. This study gives a comparison of the carbon sequestration potentials between two man-made grassland ecosystems. For many enterprises this study will aid in a management planning of man-made grassland in terms of ecosystem services, that is carbon sequestration.

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The Structure of American Foreign Policy Ideology

Jorgensen

2019

American Foreign Policy Ideology and the International Rule of Law

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(R)evolution of an Idea

Cited by 3 publications

References 8 publications

Insulin-Secreting Pituitary GH3 Cells: A Potential β-Cell Surrogate for Diabetes Cell Therapy

Insulin-Secreting Pituitary GH3 Cells: A Potential β-Cell Surrogate for Diabetes Cell Therapy

Carbon Sequestration Potentials of Man-made Grasslands under Different Pattern of Plantation Stands in West Bandung Regency, Indonesia

The Structure of American Foreign Policy Ideology

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