Quorum sensing-mediated dynamic regulation of 4-hydroxyisoleucine biosynthesis in Corynebacterium glutamicum

Ma, Fanqi; Liu, Haiyan; Shi, Feng; Xiang, Youhe; Fan, Zhengyu

doi:10.1007/s11274-023-03633-0

Cited by 3 publications

(2 citation statements)

References 38 publications

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“…In our previous studies, we utilized an isoleucine-responsive promoter P ilvBNC and a GPP promoter P CP_2836 to dynamically regulate the expression of ODHC, significantly enhancing the production of 4-hydroxyisoleucine and 5-aminolevulinic acid, respectively. , In a study focusing on high-level γ-aminobutyric acid production, Wei et al developed a series of truncated P CP_2836 promoters for fine-tuning the expression level of odhA . Furthermore, the quorum sensing system involving the P srfA promoter was employed for odhI expression, dynamically inhibiting ODHC activity and consequently increasing 4-hydroxyisoleucine production . While dynamic regulation of the TCA cycle appears superior, finding an optimal approach necessitates extensive experimentation.…”

Section: Resultsmentioning

confidence: 99%

“…68 Furthermore, the quorum sensing system involving the P srfA promoter was employed for odhI expression, dynamically inhibiting ODHC activity and consequently increasing 4hydroxyisoleucine production. 69 While dynamic regulation of the TCA cycle appears superior, finding an optimal approach necessitates extensive experimentation. The efficacy observed in the laboratory setting may not seamlessly translate to the scaling-up process, given the critical role of the TCA cycle in bacterial physiology.…”

Section: 6mentioning

confidence: 99%

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Efficient Fermentative Production of d-Alanine and Other d-Amino Acids by Metabolically Engineered Corynebacterium glutamicum

Tian,

Zhao,

et al. 2024

J. Agric. Food Chem.

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D-Amino acids (D-AAs) have wide applications in industries such as pharmaceutical, food, and cosmetics due to their unique properties. Currently, the production of D-AAs has relied on chemical synthesis or enzyme catalysts, and it is challenging to produce D-AAs via direct fermentation from glucose. We observed that Corynebacterium glutamicum exhibits a remarkable tolerance to high concentrations of D-Ala, a crucial characteristic for establishing a successful fermentation process. By optimizing mesodiaminopilmelate dehydrogenases in different C. glutamicum strains and successively deleting L-Ala biosynthetic pathways, we developed an efficient D-Ala fermentation system. The D-Ala titer was enhanced through systems metabolic engineering, which involved strengthening glucose assimilation and pyruvate supply, reducing the formation of organic acid byproducts, and attenuating the TCA cycle. During fermentation in a 5-L bioreactor, a significant accumulation of L-Ala was observed in the broth, which was subsequently diminished by introducing an L-amino acid deaminase. Ultimately, the engineered strain DA-11 produced 85 g/L D-Ala with a yield of 0.30 g/g glucose, accompanied by an optical purity exceeding 99%. The fermentation platform has the potential to be extended for the synthesis of other D-AAs, as demonstrated by the production of D-Val and D-Glu.

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Section: Resultsmentioning

confidence: 99%

Section: 6mentioning

confidence: 99%

Efficient Fermentative Production of d-Alanine and Other d-Amino Acids by Metabolically Engineered Corynebacterium glutamicum

Tian,

Zhao,

et al. 2024

J. Agric. Food Chem.

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Enhanced polysaccharide production through quorum sensing system in Cordyceps militaris

Qiao,

Chen,

Yang

2024

J Basic Microbiol

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This study aimed to enhance extracellular polysaccharide (EPS) production in Cordyceps militaris by constructing a quorum sensing (QS) system to regulate the expression of biosynthetic enzyme genes, including phosphoglucomutase, hexokinase, phosphomannomutase, polysaccharide synthase, and UDP‐glucose 4‐epimerase genes. The study found higher EPS concentrations in seven recombinant strains compared to the wild‐type C. militaris, indicating that the overexpression of key enzyme genes increased EPS production. Among them, the CM‐pgm‐2 strain exhibited the highest EPS production, reaching a concentration of 3.82 ± 0.26 g/L, which was 1.52 times higher than the amount produced by the wild C. militaris strain. Additionally, the regulatory effects of aromatic amino acids on the QS system of the CM‐pgm‐2 strain were investigated. Under the influence of 45 mg/L tryptophan, the EPS production in CM‐pgm‐2 reached 4.75 ± 0.20 g/L, representing a 1.90‐fold increase compared to wild C. militaris strains. This study provided an effective method for the large‐scale production of EPSs in C. militaris, and opened up new avenues for research into fungal QS mechanisms.

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Metabolic engineering Corynebacterium glutamicum for D-chiro-inositol production

Ji,

Jin,

Shi

2024

World J Microbiol Biotechnol

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Quorum sensing-mediated dynamic regulation of 4-hydroxyisoleucine biosynthesis in Corynebacterium glutamicum

Cited by 3 publications

References 38 publications

Efficient Fermentative Production of d-Alanine and Other d-Amino Acids by Metabolically Engineered Corynebacterium glutamicum

Efficient Fermentative Production of d-Alanine and Other d-Amino Acids by Metabolically Engineered Corynebacterium glutamicum

Enhanced polysaccharide production through quorum sensing system in Cordyceps militaris

Metabolic engineering Corynebacterium glutamicum for D-chiro-inositol production

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