Quorum sensing and butanediol fermentation affect colonization and spoilage of carrot slices by Serratia plymuthica

Wevers, Eva; Moons, Pieter; Houdt, Rob Van; Lurquin, Ine; Aertsen, Abram; Michiels, Chris W.

doi:10.1016/j.ijfoodmicro.2008.12.017

Cited by 24 publications

(19 citation statements)

References 27 publications

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“…The microbial isolates identified during the two trials were quite similar, having many common species. The identification of Pantoea agglomerans , Erwinia persicina , Serratia marcescens , and Enterobacter aerogenes in all samples confirms the importance of Enterobacteriaceae in microbial spoilage of cold stored vegetables, according to previous indications [1, 10, 20]. …”

Section: Resultssupporting

confidence: 80%

Changes in Bacterial Composition of Zucchini Flowers Exposed to Refrigeration Temperatures

Baruzzi

Cefola

Carito

et al. 2012

The Scientific World Journal

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Microbial spoilage is one of the main factors affecting the quality of fresh fruits and vegetables, leading to off-flavor, fermented aroma, and tissue decay. The knowledge of microbial growth kinetics is essential for estimating a correct risk assessment associated with consuming raw vegetables and better managing the development of spoilage microorganisms. This study shows, for the first time, that only a part of total microbial community, originally present on fresh harvested female zucchini flowers, was able to adapt itself to refrigerated conditions. Through the study of microbial growth kinetics it was possible to isolate forty-four strains belonging to twenty-two species of the generaAcinetobacter, Arthrobacter, Bacillus, Enterobacter, Erwinia, Klebsiella, Pantoea, Pseudoclavibacter, Pseudomonas, Serratia, Staphylococcus,andWeissella, suggestingEnterobacteriaceaeas potentially responsible for pistil spoilage.

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Section: Resultssupporting

confidence: 80%

Changes in Bacterial Composition of Zucchini Flowers Exposed to Refrigeration Temperatures

Baruzzi

Cefola

Carito

et al. 2012

The Scientific World Journal

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“…This pathway may contribute to virulence by alkalinizing the local environment, which favors pectate lyase activity. A related plant pathogen, Serratia plymuthica , grows well in carrot slices when the budB gene is deleted, thus, related pathogens also do not require this pathway for growth on plants [9]. Unlike P. c. subsp.…”

Section: Discussionmentioning

confidence: 99%

“…Since 3H2B and 23B can be metabolized by some bacterial species after glucose and other readily fermented carbon sources are used, it also serves as an energy storing strategy [6], [7], [8]. The 3H2B pathway may also reduce fitness in some environments; a Serratia plymuthica budB mutant is more virulent on carrot slices than wild type, even though it does not grow as well in rich medium as wild type [9].…”

Section: Introductionmentioning

confidence: 99%

The 3-Hydroxy-2-Butanone Pathway Is Required for Pectobacterium carotovorum Pathogenesis

Marquez-Villavicencio

Weber

Witherell

et al. 2011

PLoS ONE

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Pectobacterium species are necrotrophic bacterial pathogens that cause soft rot diseases in potatoes and several other crops worldwide. Gene expression data identified Pectobacterium carotovorum subsp. carotovorum budB, which encodes the α-acetolactate synthase enzyme in the 2,3-butanediol pathway, as more highly expressed in potato tubers than potato stems. This pathway is of interest because volatiles produced by the 2,3-butanediol pathway have been shown to act as plant growth promoting molecules, insect attractants, and, in other bacterial species, affect virulence and fitness. Disruption of the 2,3-butanediol pathway reduced virulence of P. c. subsp. carotovorum WPP14 on potato tubers and impaired alkalinization of growth medium and potato tubers under anaerobic conditions. Alkalinization of the milieu via this pathway may aid in plant cell maceration since Pectobacterium pectate lyases are most active at alkaline pH.

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“…Acetoin and (2R,3R)-2,3-butanediol production primarily function in alkalinization of the growth environment, which protects bacteria from unfavorable acidic conditions (Xiao and Xu 2007). Mutagenesis of budB that confers 2,3-butanediol dehydrogenase/diacetyl reductase (product of the budB gene) to convert acetoin to (2R,3R)-2,3-butanediol and diacetyl to acetoin in plant-associated Serratia plymuthica significantly reduced bacterial growth on artificial medium compared with that of wild-type bacteria (Wevers et al 2009). …”

Section: Dual Role Of Bvc-mediated Interactions Between Bacteria and mentioning

confidence: 99%

Sweet scents from good bacteria: Case studies on bacterial volatile compounds for plant growth and immunity

2015

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Beneficial bacteria produce diverse chemical compounds that affect the behavior of other organisms including plants. Bacterial volatile compounds (BVCs) contribute to triggering plant immunity and promoting plant growth. Previous studies investigated changes in plant physiology caused by in vitro application of the identified volatile compounds or the BVC-emitting bacteria. This review collates new information on BVC-mediated plant-bacteria airborne interactions, addresses unresolved questions about the biological relevance of BVCs, and summarizes data on recently identified BVCs that improve plant growth or protection. Recent explorations of bacterial metabolic engineering to alter BVC production using heterologous or endogenous genes are introduced. Molecular genetic approaches can expand the BVC repertoire of beneficial bacteria to target additional beneficial effects, or simply boost the production level of naturally occurring BVCs. The effects of direct BVC application in soil are reviewed and evaluated for potential large-scale field and agricultural applications. Our review of recent BVC data indicates that BVCs have great potential to serve as effective biostimulants and bioprotectants even under open-field conditions.

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Quorum sensing and butanediol fermentation affect colonization and spoilage of carrot slices by Serratia plymuthica

Cited by 24 publications

References 27 publications

Changes in Bacterial Composition of Zucchini Flowers Exposed to Refrigeration Temperatures

Changes in Bacterial Composition of Zucchini Flowers Exposed to Refrigeration Temperatures

The 3-Hydroxy-2-Butanone Pathway Is Required for Pectobacterium carotovorum Pathogenesis

Sweet scents from good bacteria: Case studies on bacterial volatile compounds for plant growth and immunity

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