Quinolones resistance and R-plasmids of some gram negative enteric bacilli

Daini, OA; Ogbolu, OD; Ogunledun, A

doi:10.4314/ajcem.v6i1.7394

Cited by 30 publications

(33 citation statements)

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“…All the six isolates with the plasmids weight of 854bp were also resistance to cloxacillin, amoxicillin, ampicillin and cotrimoxazole. The emergence of R-plasmids in this study could be ascribed to the indiscriminate and widespread use of antibiotics caused by over-the-counter availability of antibiotics as well as the higher exposure of people to enteric flora in places with poor sanitation 25,39,55,56 . Clinical isolates of E. coli which showed multiple drug resistance were also found to harbour plasmids with molecular sizes ranging from 2kb to 6.5 kb and a maximum 26kb.…”

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Resistant plasmid profile analysis of multidrug resistant Escherichia coli isolated from urinary tract infections in Abeokuta, Nigeria

Akingbade¹,

Balogun²,

Ojo³

et al. 2015

Afr H. Sci.

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Background: Multi-drug resistant Escherichia coli has become a major threat and cause of many urinary tract infections (UTIs) in Abeokuta, Nigeria. Objectives: This study was carried out to determine the resistant plasmids of multidrug resistant Escherichia coli isolated from (Urinary tract infections)UTIs in Abeokuta. Methods: A total of 120 Escherichia coli isolates were obtained from urine samples collected from patients attending inpatient and outpatient clinics presenting UTI; with their biodata. Antibiotics susceptibility was performed and multi-drug resistant isolates were selected for plasmid profiling. Plasmids were extracted by the alkaline lysis method, electrophoresed on 0.8% agarose gel and profiled using a gel-photo documentation system gel. Results: Escherichia coli isolates obtained shows high resistance to cloxacillin (92.5%), amoxicillin (90.8%), ampicillin (90.8%), erythromycin (75.8%), cotrimoxazole (70.0%), streptomycin (70.0%) and tetracycline (68.3%) while 85.8% and 84.2% were susceptible to gentamycin and ceftazidime respectively. Sixteen Escherichia coli strains were observed to be resistant to more than two classes of antibiotics. The resistant plasmid DNA was detectable in 6(37.5%) of the 16 multidrug resistant Escherichia coli having single sized plasmids of the same weight 854bp and were all resistant to erythromycin, cefuroxime, cloxacillin, amoxicillin, ampicillin and cotrimoxazole. Conclusion: This study has highlighted the emergence of multidrug resistant R-plasmids among Escherichia coli causing urinary tract infections in Abeokuta, Nigeria. There is a high level of resistance to many antimicrobials that are frequently used in Abeokuta, Nigeria.

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confidence: 99%

Resistant plasmid profile analysis of multidrug resistant Escherichia coli isolated from urinary tract infections in Abeokuta, Nigeria

Akingbade¹,

Balogun²,

Ojo³

et al. 2015

Afr H. Sci.

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“…E. coli are one of other common microbial floras of gastrointestinal tract of poultry [5,6]. Among the diseases some are often severe and sometimes lethal infections such as meningitis, endocarditis, urinary tract infection, septicemia, epidemic diarrhoea of adults and children [7] and yolk sac infection, omphalitis, cellulitis, swollen head syndrome, coligranuloma and colibacillosisn [8]. Enteritis caused by E. coli (colibacilliosis) is an important disease in the poultry industry because of increased mortality and decreased performance [9,10].…”

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Study on Isolation and Identification of Salmonella and Escherichia coli from Different Poultry Feeds of Savar Region of Dhaka, Bangladesh

2011

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The study was conducted aiming at the isolation and identification of Salmonella and Escherichia coli (E. coli) from different brands of poultry feeds sold in Savar, Dhaka, Bangladesh. Seven different poultry feeds were subjected to microbiological analysis. All these samples were analyzed by culturing in different media such as nutrient broth (NB), nutrient Agar (NA), SS Agar (Salmonella-Shigella Agar), BGA (brilliant-green Agar), Mac Conkey, DHL and EMB (eosin methylene blue) media. Total bacterial colonies of all the samples were counted separately on the nutrient Agar media. Hence, bacteria were counted as 9.5×105 in the feed sample C (Layer) which was found to be the highest in number among the poultry feeds. Total viable count (TVC) of Salmonella and E. coli in the feed samples were as 0 to 6.75×104 and 0 to 3.05×104 respectively. Both organisms were found in 71.43% and 57.14% of the analyzed feed samples, respectively. The highest number of Salmonella was found in sample C (Layer) feeds and that of E. coli was found in sample B (Grower) feeds. The widespread occurrence of Salmonella and E. coli in poultry feeds reinforces the need for effective control measures, hygiene in processing and handling of feeds. Keywords: Salmonella; Escherichia coli; Poultry feeds; Total viable count; Contamination; Hygiene. © 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi:10.3329/jsr.v3i2.7128 J. Sci. Res. 3 (2), 403-411 (2011)

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“…Resistance to high levels of antibiotics has been ascribed in most instances to the presence of plasmid [8][9][10][11]. Several works have associated resistance to quinolones with plasmids and some organisms that show this type of resistance are Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae and Psuedomonas aeruginosa [9,[12][13][14][15]. The increasing frequency of ciprofloxacin resistance in Enterobacteriaceae has been associated with the increasing prevalence of PMQR genes.…”

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Prevalence of Aac(6’)-Ib-Cr and Qepa Genes among Quinolone Resistant Uropathogens Isolated from Asymptomatic Female Students of a Northern University on Nigeria.

Pa¹,

Tende²,

Ro³

et al. 2017

Clin Microbiol

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Quinolones resistance and R-plasmids of some gram negative enteric bacilli

Cited by 30 publications

References 0 publications

Resistant plasmid profile analysis of multidrug resistant <i>Escherichia coli</i> isolated from urinary tract infections in Abeokuta, Nigeria

Resistant plasmid profile analysis of multidrug resistant <i>Escherichia coli</i> isolated from urinary tract infections in Abeokuta, Nigeria

Study on Isolation and Identification of <i>Salmonella</i> and <i>Escherichia coli</i> from Different Poultry Feeds of Savar Region of Dhaka, Bangladesh

Prevalence of Aac(6’)-Ib-Cr and Qepa Genes among Quinolone Resistant Uropathogens Isolated from Asymptomatic Female Students of a Northern University on Nigeria.

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