Quinolizidine Alkaloids in Seeds of Lupin Genotypes of Different Origins

Boschin, Giovanna; Annicchiarico, Paolo; Resta, Donatella; D’Agostina, Alessandra; Arnoldi, Anna

doi:10.1021/jf7037218

Cited by 58 publications

(65 citation statements)

References 21 publications

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“…Very low concentrations of quinolizidine alkaloids were observed in all the varieties (3.0 mg 100g -1 ) compared to the control Multitalia (166.4 mg 100g -1 ). The Lupanine was the most represented alkaloid (1.5 mg 100g -1 , on average of the tested varieties), whereas, Iso-lupanine was the least represented alkaloid (0.22 mg 100g -1 , on average of the tested varieties) (table 4); these results are in agreement with the observations of Muzquiz et al (1994) in the alkaloid profile of L. albus and with those of Boschin et al (2008) in nine alkaloid-poor varieties of L. albus and L. angustifolius grown in two climatically contrasting Italian sites. The chromatographic analysis allowed to identify the presence of Iso-lupanine only in L. albus; in L. angustifolius and L. luteus the Iso-lupanine content was below the instrumental limit of quantification (0.04 mg 100 g -1 ) (Tab.…”

Section: Resultssupporting

confidence: 86%

“…The analysis of the results showed that the studied samples had much lower alkaloid content than that reported by Gdala et al (1996) and Reinhard et al (2006); these values are much lower than the safe limit of toxicity (< 0.20‰), indicated for human and animal consumption by the health authorities of Great Britain, France and Australia (Boschin et al, 2008). Very low concentrations of quinolizidine alkaloids were observed in all the varieties (3.0 mg 100g -1 ) compared to the control Multitalia (166.4 mg 100g -1 ).…”

Section: Resultsmentioning

confidence: 52%

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Lupin Seed for the Crop-Livestock Food Chain

Gresta¹,

Abbate²,

Avola³

et al. 2010

Ital J Agronomy

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The ban on animal protein supplements in animal nutrition and, particularly, the increase in the demand for alternative protein sources to replace soybean meal in organic farming has stimulated research on GM-free feeds and renewed interest in grain legumes. Of these lupin appears to be one of the more interesting and promising crops for ruminant diets, due to its key role in low-input cropping systems and its high protein content. The aim of this study was to evaluate the productive and nutritional characteristics of several sweet varieties of lupin seeds cultivated in the Mediterranean region. The trial was carried out during the years 2007/08 on a volcanic soil in East Sicily, employing 8 sweet cultivars belonging to Lupinus albus, L. angustifolius and L. luteus species. Yield and its components (pod plant -1 , seed pod -1 , 1000 seed weight) were evaluated. Crude protein, fibre fractions and alkaloid content were also determined. L. albus exhibited the highest yields (2.2 t ha -1 ), with an intermediate value of pod x plant (15.4). L. luteus yielded 1.5 t ha -1 and L. angustifolius 0.5 t ha -1 . L. luteus showed higher levels of crude protein (34.2%) although the greatest protein yield per hectare was recorded in L. albus (0.7 t ha -1 ). L. angustifolius showed the highest mean values of NDF (28.1%) and ADF (25%), whereas L. luteus the lowest mean values of ADL (1.75%). Six alkaloids were detected and quantified, among which, the Iso-lupanine always was the most abundant (1.5 mg 100g -1 , on average). Lower concentrations of quinolizidine were observed in L. luteus (0.9 -1.4 mg 100g -1 ) as compared with L. angustifolius (1.5 -5.5 mg 100g -1 ) and L. albus (3.8 -7.5 mg 100g -1 ). Alkaloid content was always lower than the safe limit of toxicity (< 0.20‰) indicated for human and animal consumption.

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Section: Resultssupporting

confidence: 86%

Section: Resultsmentioning

confidence: 52%

Lupin Seed for the Crop-Livestock Food Chain

Gresta¹,

Abbate²,

Avola³

et al. 2010

Ital J Agronomy

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show abstract

“…However, identified alkaloids are uneasy using LC/MS due to no available mass spectral database on electrospray ionization (ESI). GC/MS is one of the highest sensitivity analysis techniques, whose limit of detection can easily reach picogram (10 −12 ) and provide available standard mass spectral data library [21–23]. …”

Section: Introductionmentioning

confidence: 99%

Alkaloids in Processed Rhizoma Corydalis and Crude Rhizoma Corydalis Analyzed by GC/MS

Guo

Cai

et al. 2014

Journal of Analytical Methods in Chemistry

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The alkaloids in the processed Rhizoma Corydalis and the crude Rhizoma Corydalis were qualitatively and semiquantitatively analyzed using gas chromatography-mass spectrometry (GC/MS) method. The processing herb drug procedure was carried out according to the standard method of Chinese Pharmacopoeia. The samples were extracted using Soxhlet extractor with different solvents: methanol and acetone. The extraction effect on different solvents was investigated. The results showed that 11 kinds of alkaloids were identified from the crude Rhizoma Corydalis and only two were from the processed Rhizoma Corydalis. A total of 13 kinds of alkaloids were all based on two backbones. The alkaloids in the processed sample were less than those in the crude Rhizoma Corydalis significantly, while almost the corydaline has been changed in conformation after the sample had undergone processing, which provided support for the conclusion of reducing toxicity when the herbal medicine having been undergone a traditional drugs treatment process.

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“…The total alkaloid contents of the finely-ground pooled feed samples containing either the sweet (Eureka) or bitter (SSL 10) Lupinus angustifolius cultivars were determined as described by Boschin et al (2008), with minor modifications. The sample preparation method was modified by extracting the total alkaloid content directly using a 50:50 methylene dichloride:methanol mixture (MDC:MeOH).…”

Section: Methodsmentioning

confidence: 99%

Feed preference of grower ostriches consuming diets differing in <i>Lupinus angustifolius</i> inclusion levels

Brand¹,

Engelbrecht²,

Merwe³

et al. 2018

SA J. An. Sci.

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Feed costs contribute the largest proportion of the input costs of slaughter birds in an intensive ostrich production unit. Alternative, cheaper feedstuffs, such as lupins (sweet and bitter cultivars), were therefore evaluated to determine the optimal lupin inclusion level in ostrich rations without affecting feed preference and intake. Sixty South African Black ostriches were randomly divided into ten paddocks of six birds per paddock. Three trials, with five different experimental diets, were conducted to investigate the diet preference of grower ostriches in a free-choice system. Feed and water were supplied ad libitum. The position of the diets in the successive paddocks was varied by rotating the five feed troughs in a clockwise direction, but within each paddock the position of each feeder and diet stayed the same throughout the three trials. In the first two trials, sweet (trial 1) or bitter (trial 2) lupins replaced soybean oilcake meal to have 0, 7.5, 15, 22.5, and 30% lupin inclusion levels in the diet. In trial 3 the soybean oilcake meal was replaced with either sweet or bitter lupins to have dietary inclusion levels as follows: 0% lupins, 15% sweet, 15% bitter, 30% sweet, or 30% bitter. The daily intake per group for each diet was monitored over a period of five days each. The average initial body weight of the birds was 73.6 ± 0.5 kg. No interaction was found between day and diet for the three trials and dry matter intake (DMI) did not differ between the five treatments for any of the three trials. In the second trial the birds tended to show a preference for the 7.5% bitter lupin inclusion level and discriminated against the 15% and 30% bitter lupin inclusion levels. Regression analysis of DMI on lupin inclusion rates revealed no significant trends. In conclusion, the study revealed that soybean oilcake meal can be replaced in the diets of grower ostriches by sweet lupin inclusion levels up to 30%, without any significant detrimental effect on diet preference and feed intake.

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Quinolizidine Alkaloids in Seeds of Lupin Genotypes of Different Origins

Cited by 58 publications

References 21 publications

Lupin Seed for the Crop-Livestock Food Chain

Lupin Seed for the Crop-Livestock Food Chain

Alkaloids in Processed Rhizoma Corydalis and Crude Rhizoma Corydalis Analyzed by GC/MS

Feed preference of grower ostriches consuming diets differing in <i>Lupinus angustifolius</i> inclusion levels

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