Alkaloids in Processed Rhizoma Corydalis and Crude Rhizoma Corydalis Analyzed by GC/MS

Abstract

The alkaloids in the processed Rhizoma Corydalis and the crude Rhizoma Corydalis were qualitatively and semiquantitatively analyzed using gas chromatography-mass spectrometry (GC/MS) method. The processing herb drug procedure was carried out according to the standard method of Chinese Pharmacopoeia. The samples were extracted using Soxhlet extractor with different solvents: methanol and acetone. The extraction effect on different solvents was investigated. The results showed that 11 kinds of alkaloids were identified from the crude Rhizoma Corydalis and only two were from the processed Rhizoma Corydalis. A total of 13 kinds of alkaloids were all based on two backbones. The alkaloids in the processed sample were less than those in the crude Rhizoma Corydalis significantly, while almost the corydaline has been changed in conformation after the sample had undergone processing, which provided support for the conclusion of reducing toxicity when the herbal medicine having been undergone a traditional drugs treatment process.

Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry

“…These results agreed with previous studies on this plant using GC coupled to a quadrupole mass analyzer [ 11 , 12 ] and other studies [ 9 , 25 ], while additionally we also detect coreximine. Moreover, in agreement with previous studies [ 13 , 14 , 17 , 24 ] our results suggest that the stationary phase 5% phenyl 95% dimethyl polysiloxane is also an adequate alternative to the more apolar stationary phases consisting of 100% dimethyl polysiloxane, which is also commonly used for the separation of isoquinoline alkaloids [ 11 , 12 ].…”

“…Figure 5 represents a comparative plot of the results described above. All the isoquinoline alkaloids found by GC-TOF-MS in the extract of F. capreolata were also detected using RP-HPLC-DAD-QTOF-MS. More polar alkaloids, such as quaternary alkaloids, were not detected by the first analytical platform probably due to their poor volatility that is its major drawback [ 17 ]. Moreover, the use of RP-HPLC-DAD-QTOF-MS enables us to find some alkaloids, which were not reported in this plant by other authors (e.g., compounds 2, 3, 5, 10, 13, 14, 17, 23, 24, and 26).…”

“…This type of alkaloids has been characterized by thin layer chromatography [ 9 ], gas chromatography (GC) coupled to flame ionization detector (FID) [ 10 ], and mass spectrometry (MS) [ 11 – 14 ], as well as liquid chromatography (LC) coupled to UV/Vis, diode array detection (DAD) [ 15 ], and mass spectrometry (MS) [ 16 ]. In the case of GC-MS, there is available standard mass spectral data library for the characterization of several phytochemical classes, but it is not at all complete for alkaloids in comparison with other phytochemicals and explained by their relatively poor volatilization [ 17 ]. In the case of LC-MS, there are efforts to generate spectral libraries using different MS analyzers and collision energies due to the little lack of consistency, standardization, or reproducibility as compared with GC-MS or nuclear magnetic resonance spectroscopy [ 18 ].…”

Transcriptome analysis of 20 taxonomically related benzylisoquinoline alkaloid-producing plants

“…Our study includes plant genera known to produce lesser-known morphinan alkaloids, such as Corydalis , Nandina and Thalictrum, which produce (+)-pallidine, sinoacutine, and (−)-pallidine respectively [ 21 , 22 , 47 ]. Significantly, these plants also produce a variety of aporphine alkaloids such as nantenine ( Nandina ; [ 22 ]), isocorydine ( Corydalis ; [ 14 ]) and corydine ( Thalictrum ; [ 47 ]). The biosynthetic pathways for these morphinan and aporphine alkaloids are not known, but likely rely on CYP-mediated C - C coupling of ( S )- or ( R )-reticuline.…”

<p>Traditional Chinese Medicine Jiuwei Zhenxin Granules in Treating Depression: An Overview</p>

“…A total of more than 20 alkaloids have been identified from the tubers of CYHS, including corydaline, dl-tetrahydropalmatine, protopine, L-tetraline, L-tetrahydrocoptisine (L-stylopin), dl-tetrahydroberberine, L-tetrahydrocolumbamine, corybulbine, β-herbadol, β-homoche-lidonine, coptisine, dehydrocorydaline, and corydalmine. [82][83][84][85] CYHS is currently used clinically, mainly for the treatment of drug addiction, 86 as well as for its antiaging, anxiolytic, 87 and antiplatelet aggregation 88 properties and for the treatment of depression. 89 Qi stagnation and blood stasis are primary causes of depression in the theory of TCM.…”