Quinclorac resistance: a concerted hormonal and enzymatic effort in <i>Echinochloa phyllopogon</i>

Yasuor, Hagai; Milan, Marco; Eckert, J. E.; Fischer, Albert J.

doi:10.1002/ps.2230

Cited by 60 publications

(106 citation statements)

References 57 publications

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“…For example, in the resistance to aryloxyphenoxy-propionate herbicides, significant contributions of glutathione and Cys conjugation seem to be involved (Ruiz-Santaella et al, 2006;Bakkali et al, 2007). In quinclorac resistance, insensitivity of ethylene synthesis pathways that usually respond to quinclorac and enhanced activity of b-cyanoalanine synthase were suggested (Yasuor et al, 2012). In bispyribac-sodium resistance, bispyribac-sodium-induced P450s appear involved (Yun et al, 2005), and slightly increased expression of two P450 genes (not CYP81A21 or CYP81A21) has been reported under bispyribacsodium stress (Iwakami et al, 2014a).…”

Section: Discussionmentioning

confidence: 99%

“…R plants exhibited resistance to at least nine herbicides from seven different chemical groups: sulfonylurea, triazolopyrimidine, pyrimidinyl carboxy herbicides, aryloxyphenoxypropionate, thiocarbamate, quinclorac, and clomazone (Fischer et al, 2000b;Osuna et al, 2002;Ruiz-Santaella et al, 2006;Bakkali et al, 2007;Yasuor et al, 2009Yasuor et al, , 2010Yasuor et al, , 2012. Today, there seems to be only one herbicide left to control this weed in California (Yasuor et al, 2012). Thus, elucidating the molecular mechanism of resistance is urgently required.…”

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confidence: 99%

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Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

et al. 2014

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Previous studies have demonstrated multiple herbicide resistance in California populations of Echinochloa phyllopogon, a noxious weed in rice (Oryza sativa) fields. It was suggested that the resistance to two classes of acetolactate synthase-inhibiting herbicides, bensulfuron-methyl (BSM) and penoxsulam (PX), may be caused by enhanced activities of herbicide-metabolizing cytochrome P450. We investigated BSM metabolism in the resistant (R) and susceptible (S) lines of E. phyllopogon, which were originally collected from different areas in California. R plants metabolized BSM through O-demethylation more rapidly than S plants. Based on available information about BSM tolerance in rice, we isolated and analyzed P450 genes of the CYP81A subfamily in E. phyllopogon. Two genes, CYP81A12 and CYP81A21, were more actively transcribed in R plants compared with S plants. Transgenic Arabidopsis (Arabidopsis thaliana) expressing either of the two genes survived in media containing BSM or PX at levels at which the wild type stopped growing. Segregation of resistances in the F2 generation from crosses of R and S plants suggested that the resistance to BSM and PX were each under the control of a single regulatory element. In F6 recombinant inbred lines, BSM and PX resistances cosegregated with increased transcript levels of CYP81A12 and CYP81A21. Heterologously produced CYP81A12 and CYP81A21 proteins in yeast (Saccharomyces cerevisiae) metabolized BSM through O-demethylation. Our results suggest that overexpression of the two P450 genes confers resistance to two classes of acetolactate synthase inhibitors to E. phyllopogon. The overexpression of the two genes could be regulated simultaneously by a single trans-acting element in the R line of E. phyllopogon.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

et al. 2014

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“…D. ischaemum plants resistant to quinclorac showed high β-CAS levels, in relation to the susceptible biotypes (Abdallah et al, 2006). Spraying malation associated with quinclorac on E. phyllopogon plants decreased the resistance factor from 17 to 3, in contrast to quinclorac alone (Yasuor et al, 2011). This result suggests that malation was able to inhibit the β-CAS activity on resistant E. phyllopogon plants, thus, inhibiting one of the main detoxification mechanisms of HCN (Yasuor et al, 2011).…”

Section: Auxinic Effect (Including the Production Of Cyanate)mentioning

confidence: 94%

“…E. phyllopogon biotypes that showed high β-CAS levels were resistant to the effects of cyanate, triggered after spraying quinclorac (Yasuor et al, 2011). D. ischaemum plants resistant to quinclorac showed high β-CAS levels, in relation to the susceptible biotypes (Abdallah et al, 2006).…”

Section: Auxinic Effect (Including the Production Of Cyanate)mentioning

confidence: 97%

Integrative Theory of the Mode of Action of Quinclorac: Literature Review1

Fipke

Vidal

2016

Planta daninha

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-Quinclorac is a systemic herbicide absorbed by germinating seeds, roots and leaves of seedlings. It is a selective compound for crops such as rice, canola, barley, corn, sorghum, and pasture. Quinclorac can be used to control various monocots and dicotyledonous weed species. The biochemical function of this herbicide in the plant has intrigued scientists for nearly four decades. The objectives of this review are to present evidence of three hypotheses on the biochemical functioning of quinclorac and to propose an integrative mode of action. The first theory on the mode of action of quinclorac is supported by evidence of inhibition of incorporation of C 14 -glucose into cellulose and hemicellulose, thus, affecting the cell wall synthesis. The second hypothesis suggests that quinclorac acts as an auxin in broadleaved weed species. In grass species, however, this herbicide appears to stimulate the activity of the 1-aminocyclopropane-1-carboxylate synthase enzyme and, subsequently, to increase the ethylene production; also, it seems to increase the cyanide acid content to phytotoxic levels. A third hypothesis to explain the harmful effect in some plant species is the formation of reactive oxygen species (ROS). Apparently, these processes are not mutually exclusive; therefore, an integrative theory for the action of quinclorac is suggested. It is theorized that the aforementioned biochemical activities are interconnected and can be the phytotoxic backbone to explain the herbicidal effect depending on the plant species and the plant growth stage, among other factors.Keywords: cellulose biosynthesis-inhibitor, cyanide, auxinic herbicide, reactive oxygen species. Palavras-chave: inibidor da síntese de celulose, herbicida auxínico, cianeto, espécies reativas de oxigênio. RESUMO

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“…and Galium spp.) neither increase ethylene and cyanide production, nor overproduce ROS in response to quinclorac treatment (Grossmann, 2000;Kwiatkowski, 1993, 2000;Van Eerd et al, 2005;Abdallah et al, 2006;Sunohara et al, 2010Sunohara et al, , 2011Yasuor et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Quinclorac-habituation of bean ( Phaseolus vulgaris ) cultured cells is related to an increase in their antioxidant capacity

Largo-Gosens

Castro

Alonso‐Simón

et al. 2016

Plant Physiology and Biochemistry

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The habituation of bean cells to quinclorac did not rely on cell wall modifications, contrary to what it was previously observed for the well-known cellulose biosynthesis inhibitors dichlobenil or isoxaben. The aim of the present study was to investigate whether or not the bean cells habituation to quinclorac is related to an enhancement of antioxidant activities involved in the scavenging capacity of reactive oxygen species. Treating non-habituated bean calluses with 10 μM quinclorac reduced the relative growth rate and induced a two-fold increase in lipid peroxidation. However, the exposition of quinclorac-habituated cells to a concentration of quinclorac up to 30 μM neither affected their growth rate nor increased their lipid peroxidation levels. Quinclorac-habituated calluses had significantly higher constitutive levels of three antioxidant activities (class-III peroxidase, glutathione reductase, and superoxide dismutase) than those observed in non-habituated calluses, and the treatment of habituated calluses with 30 μM quinclorac significantly increased the level of class III-peroxidase and superoxide dismutase. The results reported here indicate that the process of habituation to quinclorac in bean callus-cultured cells is related, at least partially, to the development of a stable antioxidant capacity that enables them to cope with the oxidative stress caused by quinclorac. Class-III peroxidase and superoxide dismutase activities could play a major role in the quinclorac-habituation. Changes in the antioxidant status of bean cells were stable, since the increase in the antioxidant activities were maintained in quinclorac-dehabituated cells.

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Quinclorac resistance: a concerted hormonal and enzymatic effort in Echinochloa phyllopogon

Cited by 60 publications

References 57 publications

Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

Cytochrome P450 CYP81A12 and CYP81A21 Are Associated with Resistance to Two Acetolactate Synthase Inhibitors in Echinochloa phyllopogon

Integrative Theory of the Mode of Action of Quinclorac: Literature Review1

Quinclorac-habituation of bean ( Phaseolus vulgaris ) cultured cells is related to an increase in their antioxidant capacity

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