Quercetin–Resveratrol Combination for Prostate Cancer Management in TRAMP Mice

Singh, Chandra K.; Chhabra, Gagan; Ndiaye, Mary A.; Siddiqui, Imtiaz A.; Panackal, Jennifer E.; Mintie, Charlotte A.; Ahmad, Nihal

doi:10.3390/cancers12082141

Cited by 33 publications

(27 citation statements)

References 61 publications

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“…In a very recent study from our lab, we evaluated the effect of quercetin and resveratrol combination in the diet in transgenic adenocarcinoma of the mouse prostate (TRAMP) and have found significant inhibition in PCa development and progression. This is a promising observation; however, this study did not evaluate to assess the level of Zn in response to quercetin and resveratrol combination [103]. These anti-cancer abilities of quercetin along with its ability to increase Zn uptake in the cells indicate that quercetin should continue to be studied for its applications in human PCa.…”

Section: Quercetinmentioning

confidence: 94%

“…Resveratrol's effects against cancers including its ability to inhibit key signaling events associated with tumor development and progression, and multidrug resistance, make it is a unique natural agent in cancer management [99,100]. Recent studies on resveratrol alone or combination were shown to have promising anticancer effects against PCa [101][102][103]. However, most of these studies have not evaluated the effect of resveratrol on prostatic Zn levels.…”

Section: Resveratrolmentioning

confidence: 99%

“…It has been shown that quercetin inhibits proliferation in multiple cancer cell lines including PCa. Several animal studies have also demonstrated anti-PCa effects of quercetin [103,105,106]. Quercetin has been found to induce apoptosis and necrosis by affecting mitochondrial integrity and homeostasis of reactive oxygen species (ROS) depending on the genetic makeup of PCa cells [107].…”

Section: Quercetinmentioning

confidence: 99%

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Dietary Phytochemicals in Zinc Homeostasis: A Strategy for Prostate Cancer Management

et al. 2021

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Studies have suggested an important role of the trace element zinc (Zn) in prostate biology and functions. Zn has been shown to exist in very high concentrations in the healthy prostate and is important for several prostatic functions. In prostate cancer (PCa), Zn levels are significantly decreased and inversely correlated with disease progression. Ideally, restoration of adequate Zn levels in premalignant/malignant prostate cells could abort prostate malignancy. However, studies have shown that Zn supplementation is not an efficient way to significantly increase Zn concentrations in PCa. Based on a limited number of investigations, the reason for the lower levels of Zn in PCa is believed to be the dysregulation of Zn transporters (especially ZIP and ZnT family of proteins), metallothioneins (for storing and releasing Zn), and their regulators (e.g., Zn finger transcription factor RREB1). Interestingly, the level of Zn in cells has been shown to be modulated by naturally occurring dietary phytochemicals. In this review, we discussed the effect of selected phytochemicals (quercetin, resveratrol, epigallocatechin-3-gallate and curcumin) on Zn functioning and proposes that Zn in combination with specific dietary phytochemicals may lead to enhanced Zn bioaccumulation in the prostate, and therefore, may inhibit PCa.

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Section: Quercetinmentioning

confidence: 94%

Section: Resveratrolmentioning

confidence: 99%

Section: Quercetinmentioning

confidence: 99%

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Dietary Phytochemicals in Zinc Homeostasis: A Strategy for Prostate Cancer Management

et al. 2021

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“…Ye et al [ 207 ] and Wang et al [ 208 ] reported that resveratrol suppresses the proliferation of prostate cancer cells, promotes apoptosis, and inhibits the expression of androgen receptors. In vivo studies [ 209 , 210 ] demonstrated that a combination of curcumin and resveratrol exhibited strong modulatory potential against prostate carcinogenesis, while a combination of quercetin and resveratrol may serve as a potential novel regimen for the prevention and/or treatment of prostate cancer.…”

Section: The Role Of Nutritional Factors and Foodstuffs On Prostatmentioning

confidence: 99%

Dietary Factors and Prostate Cancer Development, Progression, and Reduction

et al. 2021

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Due to the constantly increasing number of cases, prostate cancer has become one of the most important health problems of modern societies. This review presents the current knowledge regarding the role of nutrients and foodstuff consumption in the etiology and development of prostate malignancies, including the potential mechanisms of action. The results of several in vivo and in vitro laboratory experiments as well as those reported by the clinical and epidemiological research studies carried out around the world were analyzed. The outcomes of these studies clearly show the influence of both nutrients and food products on the etiology and prevention of prostate cancer. Consumption of certain nutrients (saturated and trans fatty acids) and food products (e.g., processed meat products) leads to the disruption of prostate hormonal regulation, induction of oxidative stress and inflammation, and alteration of growth factor signaling and lipid metabolism, which all contribute to prostate carcinogenesis. On the other hand, a high consumption of vegetables, fruits, fish, and whole grain products exerts protective and/or therapeutic effects. Special bioactive functions are assigned to compounds such as flavonoids, stilbenes, and lycopene. Since the influence of nutrients and dietary pattern is a modifiable risk factor in the development and prevention of prostate cancer, awareness of the beneficial and harmful effects of individual food ingredients is of great importance in the global strategy against prostate cancer.

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“…Protein from tumor tissues was isolated in 1X RIPA lysis buffer (Millipore), and quantified using Pierce BCA Protein assay kit (Thermo Scientific) as described earlier (25). Wes ProteinSimple, a capillary-based immunodetection system was used for protein analysis.…”

Section: Quantitative Immunodetection Analysis Using Proteinsimplementioning

confidence: 99%

Genetic Manipulation of Sirtuin 3 Causes Alterations of Key Metabolic Regulators in Melanoma

et al. 2021

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The mitochondrial sirtuin SIRT3 plays key roles in cellular metabolism and energy production, which makes it an obvious target for the management of cancer, including melanoma. Previously, we have demonstrated that SIRT3 was constitutively upregulated in human melanoma and its inhibition resulted in anti-proliferative effects in vitro in human melanoma cells and in vivo in human melanoma xenografts. In this study, we expanded our data employing knockdown and overexpression strategies in cell culture and mouse xenografts to further validate and establish the pro-proliferative function of SIRT3 in melanocytic cells, and its associated potential mechanisms, especially focusing on the metabolic regulation. We found that short-hairpin RNA (shRNA) mediated SIRT3 knockdown in G361 melanoma cells showed diminished tumorigenesis in immunodeficient Nu/Nu mice. Conversely, SIRT3 overexpressing Hs294T melanoma cells showed increased tumor growth. These effects were consistent with changes in markers of proliferation (PCNA), survival (Survivin) and angiogenesis (VEGF) in xenografted tissues. Further, in in vitro culture system, we determined the effect of SIRT3 knockdown on glucose metabolism in SK-MEL-2 cells, using a PCR array. SIRT3 knockdown caused alterations in a total of 37 genes involved in the regulation and enzymatic pathways of glucose (32 genes) and glycogen (5 genes) metabolism. Functions annotation of these identified genes, using the ingenuity pathway analysis (IPA), predicted cumulative actions of decreased cell viability/proliferation, tumor growth and reactive oxygen species (ROS), and increased apoptosis in response to SIRT3 knockdown. Further, IPA gene network analysis of SIRT3 modulated genes revealed the interactions among these genes in addition to several melanoma-associated genes. Sirtuin pathway was identified as one of the top canonical pathways showing the interaction of SIRT3 with metabolic regulatory genes along with other sirtuins. IPA analysis also predicted the inhibition of HIF1α, PKM, KDM8, PPARGC1A, mTOR, and activation of P53 and CLPP; the genes involved in major cancer/melanoma-associated signaling events. Collectively, these results suggest that SIRT3 inhibition affects cellular metabolism, to impart an anti-proliferative response against melanoma.

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Quercetin–Resveratrol Combination for Prostate Cancer Management in TRAMP Mice

Cited by 33 publications

References 61 publications

Dietary Phytochemicals in Zinc Homeostasis: A Strategy for Prostate Cancer Management

Dietary Phytochemicals in Zinc Homeostasis: A Strategy for Prostate Cancer Management

Dietary Factors and Prostate Cancer Development, Progression, and Reduction

Genetic Manipulation of Sirtuin 3 Causes Alterations of Key Metabolic Regulators in Melanoma

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