Quercetin Promotes Increase in the Fertility Rate of Frozen Semen of Stallions Considered Sensitive to Freezing

Silva, L.F.M.C.; Araújo, Endrigo Adonis Braga de; Oliveira, Sidnei Nunes de; Dalanezi, Felipe Morales; Andrade, Luciana Segura de; Carneiro, João Alexandre Matos; Rodrigues, Luciana; Hayashi, R. M.; Crespilho, André Maciel; Dell’Aqua, C.P.F.; Dell’Aqua, J.A.; Papa, Frederico Ozanam

doi:10.1016/j.jevs.2018.05.049

Cited by 4 publications

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“…Quercetin is the major functional compound present in OPE [21]. It has been found that quercetin increases sperm motility and quantity, stimulates reproductive organs and enhances sperm storage lifetimes in cryopreservation [22232425262728]. However, quercetin has also been reported to have negative effects such as reducing the number of litters in female mice, and conflicting reports have also reported reduced sperm motility [2930].…”

Section: Introductionmentioning

confidence: 99%

Onion peel extract and its constituent, quercetin inhibits human Slo3 in a pH and calcium dependent manner

Wijerathne

Kim

et al. 2019

Korean J Physiol Pharmacol

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Sperm function and male fertility are closely related to pH dependent K + current (KSper) in human sperm, which is most likely composed of Slo3 and its auxiliary subunit leucine-rich repeat-containing protein 52 (LRRC52). Onion peel extract (OPE) and its major active ingredient quercetin are widely used as fertility enhancers; however, the effect of OPE and quercetin on Slo3 has not been elucidated. The purpose of this study is to investigate the effect of quercetin on human Slo3 channels. Human Slo3 and LRRC52 were co-transfected into HEK293 cells and pharmacological properties were studied with the whole cell patch clamp technique. We successfully expressed and measured pH sensitive and calcium insensitive Slo3 currents in HEK293 cells. We found that OPE and its key ingredient quercetin inhibit Slo3 currents. Inhibition by quercetin is dose dependent and this degree of inhibition decreases with elevating internal alkalization and internal free calcium concentrations. Functional moieties in the quercetin polyphenolic ring govern the degree of inhibition of Slo3 by quercetin, and the composition of such functional moieties are sensitive to the pH of the medium. These results suggest that quercetin inhibits Slo3 in a pH and calcium dependent manner. Therefore, we surmise that quercetin induced depolarization in spermatozoa may enhance the voltage gated proton channel (Hv1), and activate non-selective cation channels of sperm (CatSper) dependent calcium influx to trigger sperm capacitation and acrosome reaction.

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