Quercetin Potently Reduces Biofilm Formation of the Strain Pseudomonas aeruginosa PAO1 in vitro

Abstract

This work was focused on in vitro evaluation of anti-biofilm and anti-quorum sensing effects of four selected flavonoid compounds /(+)-catechin, caffeic acid, quercetin and morin/ using the strain Pseudomonas aeruginosa PAO1. At a concentration of 0.5 MIC quercetin was the only compound found to potently reduce both P. aeruginosa biofilm formation (95%) and its twitching motility. The chemical scaffold of quercetin, a common dietary polyphenol, may actually inspire development of novel and more effective medicinal agents targeting P. aeruginosa, the bacterium well known for its resistance.

Quercetin potentiates meropenem activity among pathogenic carbapenem‐resistantPseudomonas aeruginosaandAcinetobacter baumannii

“…These two observations indicated that decreased expression of bla NDM and mexB / adeB played only a secondary role in the development of synergy between quercetin and meropenem. Since quercetin and its derivatives mostly demonstrated antibacterial activity against P. aeruginosa and A. baumannii by either disrupting biofilm formation or enhancing bacterial membrane permeabilization, it could be anticipated that quercetin treatment would achieved transcriptional inhibition of bla NDM and mexB / adeB as a consequence of perimembrane environment alteration and changes in quorum sensing (Pejin et al ; Kim et al ).…”

Iranian propolis efficiently inhibits growth of oral streptococci and cancer cell lines

“…On the contrary, Tunisian propolis inhibited the oral biofilm at a concentration of higher than MIC compared to other studies [25]. According to reports, Quercetin inhibited the anti-biofilm activity against bacterial pathogens such as Pseudomonas aeruginosa [16, 17], Staphylococcus aureus [18] , Streptococci mutans [26] and Listeria monocytogenes [19] indicating that Q had a potential of controlling the biofilm formation and may inhibit biofilm through specific mechanisms such as the initial adhesion or production of extracellular polymeric substances [19]. Therefore, the biofilm inhibitory effect of Q in the present study might be through similar mechanisms.…”

“…Quercetin has a wide range of pharmacological properties such as the anti-inflammation and anti-oxidation; hence, it can be beneficial to the human health. Previous studies on bioactive effects of quercetin revealed that it could inhibit the growth of different microorganisms, especially gram-positive bacteria ( Bacillus subtilis , Micrococcus luteus , Staphylococcus aureus and Staphylococcus epidermidis) and gram negative bacilli ( Escherichia coli and Pseudomonas aeruginosa ) [16–19]. Quercetin applied anti-proliferative and antitumor activities via various mechanisms such as cell cycle arrest during G0/G1 or G2/M phases of the cell cycle in leukemia, breast carcinoma and esophageal adenocarcinoma cells [20–22].…”

The impact of flavonoids-rich Ziziphus jujuba Mill. Extract on Staphylococcus aureus biofilm formation

“…Current research shows that the removal of adhesion proteins, the increase in bacterial pH, and the destruction of the bacterial quorum sensing system are the key factors for removing S. aureus biofilms [24,25,27,35]. Studies have confirmed that scavenging free radicals and reactive oxygen species from flavonoids can effectively change the bacterial pH environment, suggesting that this is one of the mechanisms by which flavonoids can remove biofilms.…”