Quercetin manipulates the expression of genes involved in the reactive oxygen species (ROS) process in chicken heterophils

Abstract

Chicken heterophils generate reactive oxygen species (ROS) molecules to defend against invading pathogens. The present study examined effects of quercetin on chicken heterophils. Heterophils were stimulated with PBS, 50 µM quercetin (QH), PMA or Escherichia coli (EC) and the resulting intracellular ROS molecules were determined. Flow cytometry results showed that cells stimulated with QH, PMA and EC had a higher ROS production. Increases in intracellular ROS molecules were identified in all treatment groups by fluorescence microscopy. Determination of the ability of quercetin to manipulate mRNA expression of ROS subunits was assessed using real-time RT-PCR. Quercetin and other stimulants up-regulated the majority of genes involved in ROS production: CYBB (NOX2), NCF1 (p47phox), NCF2 (p67phox), NOX1 and RAC2. The antioxidant property of QH was explored by measuring mRNA expression of CAT and SOD1. The data indicate increased levels of CAT with all treatments; however, only QH attenuated the expression of the SOD1 gene. To further investigate the effects of ROS-driven inflammation or cell death, IL6, CASP8 and MCL1 genes were preferentially tested. The inflammatory gene (IL6) was profoundly down-regulated in the QH- and PMA-treated groups while EC induced a strikingly high IL6 expression level. Investigation of the known apoptotic (CASP8) and anti-apoptotic (MCL1) genes found down-regulation of CASP8 in the QH- and PMA-treated groups which were contradicted to the MCL1 gene. In conclusion, quercetin can enhance ROS production by regulating the expression of genes involved in ROS production as well as in subsequent processes.

Comparative Pharmacognosy, Chemical Profile and Antioxidant Activity of Extracts from Phania matricarioides (Spreng.) Griseb. Collected from Different Localities in Cuba

“…In particular, phenolic compounds have been shown to be effective agents in scavenging reactive species responsible for inducing oxidative stress. In concordance with chemical studies, quercetin was identified in the three extracts, which has been noted in the literature for its antioxidant activities [35,36], and anticancer [37], neuroprotection [38], hepatoprotection [39], anti-inflammatory [40], and antiparasitic [41,42] properties. Among the P. matricarioides extracts, higher antioxidant activity was observed in the PmC extract, which also displayed the higher concentration of quercetin according to the chemical profile as shown in the quantitative map.…”

In-Vitro Antioxidant and Pharmacognostic Studies of Phaseolus vulgaris (Linn) Seed Coat

“…Scavenging of DPPH is one of the imperative parameters to assess the antioxidant effect of crude extracts. In this study extract exhibited higher percentage of DPPH scavenging activity and the study suggested plant extract contain flavonoids and related polyphenols [38].…”

Effects of dietary quercetin on the antioxidative status and cecal microbiota in broiler chickens fed with oxidized oil

“…In the present study, quercetin reversed the decrease of HO-1 mRNA expression in the ileal mucosa induced by oxidized oil. NADPH oxidases ( NOX ) were the major sources of superoxide anions and mainly expressed in phagocytes, including chicken heterophils ( Katsuyama, 2010 ; Nambooppha et al., 2018 ). In our study, the group treated with quercetin (800 ppm) showed significant downregulation in the mRNA expression of NOX2 compared with the oxidized oil treatment group.…”