Quercetin inhibits cell viability, migration and invasion by regulating miR-16/HOXA10 axis in oral cancer

Zhao, Junfang; Fang, Zheng; Zha, Zhian; Sun, Qiang; Wang, Haibin; Sun, Minglei; Qiao, Bin

doi:10.1016/j.ejphar.2019.01.006

Cited by 82 publications

(67 citation statements)

References 20 publications

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“…It has been reported that quercetin blocks the progression of diverse human tumors, such as oral cancer, breast cancer, and NSCLC . In this work, the function of quercetin in reversing the radioresistance of NSCLC cells was studied.…”

Section: Discussionmentioning

confidence: 99%

“…For instance, miR‐16‐5p enhances the radiosensitivity of prostate cancer cells via modulating the Cyclin D1/E1‐pRb‐E2F1 signaling pathway . Interestingly, quercetin exerts anti‐cancer effects via modulating the expression of multiple miRNAs, such as miR‐27a, miR‐22, miR‐16, and miR‐146a . Specifically, quercetin impedes the proliferation of breast cancer cells via upregulating the expression of miR‐146a .…”

Section: Discussionmentioning

confidence: 99%

“…Specifically, quercetin impedes the proliferation of breast cancer cells via upregulating the expression of miR‐146a . In oral cancer, quercetin up‐regulates miR‐16 expression and down‐regulates HOXA10 expression to repress cell survival, migration, and invasion . Therefore, it was investigated here whether quercetin can modulate the expression of miR‐16‐5p in NSCLC cells.…”

Section: Discussionmentioning

confidence: 99%

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Quercetin radiosensitizes non‐small cell lung cancer cells through the regulation of miR‐16‐5p/WEE1 axis

et al. 2020

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Background Quercetin, a widely distributed bioflavonoid, plays a role in combating diverse human cancers including non‐small cell lung cancer (NSCLC). However, the role of quercetin in reversing the radioresistance of NSCLC cells and its underlying mechanism are far from being elucidated. Method Radiation‐resistant NSCLC cell lines were established. Quantitative real‐time PCR (qRT‐PCR) was used to detect the expression of miR‐16‐5p and WEE1 G2 checkpoint kinase (WEE1) mRNA in radiation‐resistant cells. After being treated with different concentrations of quercetin and different doses of X‐ray, cell proliferation and apoptosis were monitored by CCK‐8 assay, colony formation assay, and flow cytometry, respectively. Ultimately, the targeting relationship between miR‐16‐5p and WEE1 was verified via a dual fluorescent reporter gene assay. Results The expression of miR‐16‐5p was down‐regulated in radiation‐resistant cells, while the expression of WEE1 was up‐regulated. Quercetin enhanced the radiosensitivity of NSCLC cells in a dose‐ and time‐dependent manner. Furthermore, quercetin treatment increased the expression of miR‐16‐5p and decreased the expression of WEE1. The function of quercetin was reversed by miR‐16‐5p inhibitors or the transfection of WEE1 overexpressing plasmids. Conclusion In conclusion, quercetin enhanced the radiosensitivity of NSCLC cells via modulating the expression of miR‐16‐5p and WEE1.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Quercetin radiosensitizes non‐small cell lung cancer cells through the regulation of miR‐16‐5p/WEE1 axis

et al. 2020

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“…Quercetin has been well demonstrated to be an anticancer agent and has shown efficacy in several glioma cell lines . Although it has not been shown to suppress glioma growth in vivo, this drug has been demonstrated to be accumulated in the rat brain tissues , supporting its potential usefulness as an agent against glioma.…”

Section: Discussionmentioning

confidence: 99%

Quercetin depletes intracellular Ca²⁺ stores and blunts ATP‐triggered Ca²⁺ signaling in bEnd.3 endothelial cells

Chen

Hour

Shiao

et al. 2019

Fundamemntal Clinical Pharma

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Quercetin is a flavonol polyphenol widely found in many vegetables, grains, and fruits. Quercetin has been shown to inhibit proliferation and invasion of various glioma cells and is regarded as a potential anticancer agent against glioma. However, whether and how this drug could affect brain blood vessels and endothelial cells (EC) are less understood. Further, there is hitherto no report on how quercetin affects brain EC Ca2+ homeostasis. In this report, we investigated the effects of quercetin on Ca2+ homeostasis in mouse brain bEnd.3 EC. We demonstrated that quercetin raised cytosolic Ca2+ level in a concentration‐dependent manner. Quercetin‐triggered Ca2+ signal composed of both internal Ca2+ release and extracellular Ca2+ influx. Quercetin caused Ca2+ release from the endoplasmic reticulum, and consistently, inhibition of inositol 1,4,5‐trisphosphate receptor (IP3R) by xestospongin C (XeC) suppressed quercetin‐triggered Ca2+ release. Quercetin also caused Ca2+ release from lysosomes, an observation in concordance with the inhibition of quercetin‐triggered Ca2+ release by trans‐Ned‐19, a blocker of two‐pore channels. As quercetin depleted intracellular Ca2+ storage, it suppressed ATP‐induced Ca2+ release and thereby blunted ATP‐triggered Ca2+ signaling. In addition, quercetin co‐treatment significantly suppressed ATP‐stimulated nitric oxide release. Our work therefore showed, for the first time, quercetin perturbed intracellular Ca2+ stores and strongly suppressed ATP‐triggered response in bEnd.3 cells.

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“…Downstream, quercetin inhibited the expression and activities of MMP‐2 and MMP‐9, while also reducing the levels of MMP‐7, MMP‐10, vascular endothelial growth factor, nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NF‐kB) p65, inducible nitric oxide synthase, cyclooxygenase‐2 (COX‐2), nuclear factor of kappa light polypeptide gene enhancer in B‐cells inhibitor (IκB‐α), IκB‐α/β, p‐IκB kinase α/β, focal adhesion kinase, son of sevenless homolog‐1, and growth factor receptor‐bound protein‐2, and promoting the expression of Rho and Rho‐associated coiled‐coil containing protein kinase‐1. Recently, researchers found that microRNA (miR‐16) participates in regulating cell proliferation and apoptosis by combining with serine‐threonine protein kinase 3 (AKT3) and BCL2‐like protein 2 in SCC, and also that miRNAs regulate homeobox A10, while miR‐16 reverses quercetin effects on HSC‐6 and SCC‐9 cell viability, migration, and invasion in SCC‐9 (Zhao et al, ).…”

Section: Flavonolsmentioning

confidence: 99%

Effects of flavonoids on tongue squamous cell carcinoma

Gutiérrez-Venegas

Sánchez-Carballido

Suárez

et al. 2019

Cell Biology International

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Squamous cell carcinoma (SCC) of the tongue is associated with tobacco use, alcohol abuse, and human papillomavirus (HPV) infections. While clinical outcomes have recently improved for HPV‐positive patients in general, 50% of patients suffering from tongue cancer die within 5 years of being diagnosed. Flavonoids are secondary plant metabolites with a wide range of biological activities including antioxidant, anti‐inflammatory, and anticancer activities. Flavonoids have generated high interest as therapeutic agents owing to their low toxicity and their effects on a large variety of cancer cell types. In this literature review, we evaluate the actions of flavonoids on SCC of the tongue demonstrated in both in vivo and in vitro models.

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Quercetin inhibits cell viability, migration and invasion by regulating miR-16/HOXA10 axis in oral cancer

Cited by 82 publications

References 20 publications

Quercetin radiosensitizes non‐small cell lung cancer cells through the regulation of miR‐16‐5p/WEE1 axis

Quercetin radiosensitizes non‐small cell lung cancer cells through the regulation of miR‐16‐5p/WEE1 axis

Quercetin depletes intracellular Ca²⁺ stores and blunts ATP‐triggered Ca²⁺ signaling in bEnd.3 endothelial cells

Effects of flavonoids on tongue squamous cell carcinoma

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Quercetin inhibits cell viability, migration and invasion by regulating miR-16/HOXA10 axis in oral cancer

Cited by 82 publications

References 20 publications

Quercetin radiosensitizes non‐small cell lung cancer cells through the regulation of miR‐16‐5p/WEE1 axis

Quercetin radiosensitizes non‐small cell lung cancer cells through the regulation of miR‐16‐5p/WEE1 axis

Quercetin depletes intracellular Ca2+ stores and blunts ATP‐triggered Ca2+ signaling in bEnd.3 endothelial cells

Effects of flavonoids on tongue squamous cell carcinoma

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Quercetin depletes intracellular Ca²⁺ stores and blunts ATP‐triggered Ca²⁺ signaling in bEnd.3 endothelial cells