Quercetin Inhibits Cell Migration and Invasion in Human Osteosarcoma Cells

Abstract

Background/Aims: Osteosarcoma is a malignant tumor associated with high mortality; however, no effective therapies for the disease have been developed. Several studies have focused on elucidating the pathogenesis of osteosarcoma and have aimed to develop novel therapies for the disease. Quercetin is a vital dietary flavonoid that has been shown to have a variety of anticancer effects, as it induces cell cycle arrest, apoptosis, and differentiation and is involved in cell adhesion, metastasis and angiogenesis. Herein, we aimed to investigate the effects of quercetin on osteosarcoma migration and invasion in vitro and in vivo and to explore the molecular mechanisms underlying its effects on osteosarcoma migration and invasion. Methods: Cell viability, cell cycle activity and cell apoptosis were measured using CCK-8 assay and flow cytometry, and cell migration and invasion were evaluated by wound healing and transwell assays, respectively. The mRNA and protein expression levels of several proteins of interest were assessed by real-time quantitative PCR and western blotting, respectively. Moreover, a nude mouse model of human osteosarcoma lung metastasis was established to assess the anti-metastatic effects of quercetin in vivo. Results: We noted no significant differences in cell cycle activity and apoptosis between HOS and MG63 cells and control cells. Treatment with quercetin significantly attenuated cell migration and invasion in HOS and MG63 cells compared with treatment with control medium. Moreover HIF-1α, VEGF, MMP2, and MMP9 mRNA and protein expression levels were significantly downregulated in HOS cells treated with quercetin compared with HOS cells treated with controls. Additionally, treatment with quercetin attenuated metastatic lung tumor formation and growth in the nude mouse model of osteosarcoma compared with treatment with controls. Conclusion: Our findings regarding the inhibitory effects of quercetin on cell migration and invasion suggest that quercetin may have potential as a therapy for human osteosarcoma.

Allicin Inhibits Proliferation and Invasion in Vitro and in Vivo via SHP-1-Mediated STAT3 Signaling in Cholangiocarcinoma

“…Accumulating evidence has shown that natural or dietary agents may be novel therapies for cancer [35,38]. Here, our present study highlights the anti-tumor effects of a natural product, allicin, which mainly involves the suppression of SHP-1-mediated STAT3 signaling.…”

Molecular Regulation of Bone Metastasis Pathogenesis

“…Current stuies support that osteolysis inhibitors might be another treatment to decrease tumour burden [118-120]. Bisphosphonates is a famous bone-targeted agents inhibiting osteoclast activity and decreasing tumour burden [121].…”

“…Several therapy, such as osteroprotegerin, RANK-Fc, PTHrp antibodies, and Vitamin-D analogues, are under phase I to III studies. Other target therapy, such as anti-MMP-9,anti-IGF-1R, Quercetin, Osthole and activating aromatic hydrocarbon receptor, were reported in rescent [120, 122-126]. Current treatments mainly target the RANK-RANKL pathway and PTHrP signaling.…”

Salidroside inhibits migration and invasion of poorly differentiated thyroid cancer cells

“…The basement membrane and extracellular matrix are protective barriers for cancer cells to metastasize . MMPs, the family of metal matrix enzymes function to digest collagen, which makes cancer cells move to other sites, and are often found upregulated during cancer progression . MMP9 primarily degrades collagen type IV, the main component of the basement membrane .…”