Salidroside inhibits migration and invasion of poorly differentiated thyroid cancer cells

Abstract

Background No effective treatment is currently available for poorly differentiated thyroid cancer which is resistant to radioiodine, especially with migration and invasion. A great number of researches have revealed the anticancer effects of salidroside, but none have studied the effects of salidroside on thyroid cancer. This study aimed to investigate the effect of salidroside on migration and invasion of poorly differentiated thyroid cancer cells. Methods The effects of salidroside on migration, invasion and apoptosis of poorly differentiated thyroid cancer WRO cells and normal thyroid follicular epithelial Nthy‐ori 3‐1 cells were measured by wound‐healing assay, transwell migration/invasion assay and flow cytometry, respectively. The expression levels of MMP2 and MMP9 at RNA and protein levels in WRO cells were detected by qRT‐PCR and western blot. The phosphorylation levels of Janus kinase 2 (JAK2), signal transducer and activator of transcription 3 (STAT3) and the apoptosis‐related protein levels of Bax, cleaved caspase 3 and Bcl‐2 were assessed by western blot. Results Salidroside significantly suppressed migration/invasion and induced apoptosis in poorly differentiated thyroid cancer WRO cells. We further illustrated that salidroside significantly inhibited expressions of MMP2 and MMP9 at mRNA and protein levels and the phosphorylation activation of JAK2/STAT3 in WRO cells. In addition, salidroside increased expressions of pro‐apoptotic factors (Bax and cleaved caspase 3) and decreased expression of anti‐apoptotic factor (Bcl‐2) significantly in WRO cells. Conclusion The present study demonstrates that salidroside inhibits migration and invasion of WRO cells (a kind of poorly differentiated cancer cell line) significantly, which might be via suppressing JAK2‐STAT3 signaling pathway.

Salidroside Ameliorates Radiation Damage by Reducing Mitochondrial Oxidative Stress in the Submandibular Gland

“…In normal cells, salidroside plays important roles in anti-inflammation, anti-stress, antioxidant, anti-aging, and anti-viral activities [ 46 ], while in cancer cells, salidroside inhibits cell proliferation and induces cell apoptosis, including urinary bladder cancer, breast cancer, gastric and colorectal cancer, lung cancer, nasopharyngeal carcinoma (NPC), and sarcoma [ 46 , 47 ]. Meanwhile, salidroside had no cytotoxic effect on the normal cells, such as thyroid follicular epithelial cells and human mammary epithelial cells, when it inhibited the cellular migration and invasion in poorly differentiated thyroid cancer cells or breast cancer cells with the drug concentrations of 5~40 µM [ 48 , 49 ]. Furthermore, salidroside could inhibit the growth of NPC xenografts, transplanted solid Ehrlich adenocarcinoma, and Pliss lymphosarcoma [ 47 , 50 ].…”

ANGPTL1 is a potential biomarker for differentiated thyroid cancer diagnosis and recurrence

“…Cell migration and invasion are important factors in tumor metastasis that are associated with the prognosis for patients with thyroid cancer ( 25 , 26 ). The ANGPTL1 mRNA levels were compared between DTC patients with N0 (no lymphatic metastasis) and N1 (lymph node metastasis) stage using TCGA data.…”

Salidroside ameliorates severe acute pancreatitis-induced cell injury and pyroptosis by inactivating Akt/NF-κB and caspase-3/GSDME pathways

“…Interestingly, other studies have reported conflicting results. In thyroid cancer WRO cells, Sal significantly restrained cell migration and invasion, and induced apoptosis by activating JAK2/STAT3 pathway and promoting cleaved caspase-3 level [ 48 ]. These findings suggest that Sal could regulate Akt/NF-κB and Caspase-3/GSDME pathways, but the activation or inactivation might differ in different diseases.…”