Quercetin-induced cardioprotection against doxorubicin cytotoxicity

Abstract

BackgroundCancer has continually been the leading cause of death worldwide for decades. Thus, scientists have actively devoted themselves to studying cancer therapeutics. Doxorubicin is an efficient drug used in cancer therapy, but also produces reactive oxygen species (ROS) that induce severe cytotoxicity against heart cells. Quercetin, a plant-derived flavonoid, has been proven to contain potent antioxidant and anti-inflammatory properties. Thus, this in vitro study investigated whether quercetin can decrease doxorubicin-induced cytotoxicity and promote cell repair systems in cardiomyocyte H9C2 cells.ResultsProteomic analysis and a cell biology assay were performed to investigate the quercetin-induced responses. Our data demonstrated that quercetin treatment protects the cardiomyocytes in a doxorubicin-induced heart damage model. Quercetin significantly facilitated cell survival by inhibiting cell apoptosis and maintaining cell morphology by rearranging the cytoskeleton. Additionally, 2D-DIGE combined with MALDI-TOF MS analysis indicated that quercetin might stimulate cardiomyocytes to repair damage after treating doxorubicin by modulating metabolic activation, protein folding and cytoskeleton rearrangement.ConclusionBased on a review of the literature, this study is the first to report detailed protective mechanisms for the action of quercetin against doxorubicin-induced cardiomyocyte toxicity based on in-depth cell biology and proteomic analysis.

Effect of quercetin glucosides from Allium extracts on HepG2, PC‑3 and HT‑29 cancer cell lines

“…The results are concordant with previous studies (22,23), which suggested that Quercetin glucosides in food products have been reported to exhibit chemopreventive activities. The lowest IC 50 value for onion extracts in cancer cell lines has been observed for those extracts with the highest presence of quercetin glucosides (22,23). This indicates that quercetin glucosides may be the most potent bioactive compound in onion extracts.…”

“…Quercetins have been reported to exhibit anti-proliferative activity towards cancer cells in vitro and in vivo (15,20,21,23). Quercetin glucosides are considered to act as antioxidants in the genus Allium (22,23). The combination of quercetin glucosides may contribute to the anti-proliferative activity of onion extracts on cancer cells (20)(21)(22)(23).…”

Recovery of adriamycin induced mitochondrial dysfunction in liver by selenium

“…It was shown that the liver has higher cytochrome-P450 reductase level than does cardiac tissue (Myers et al 1982). The semiquinone subsequently reacts with oxygen, iron, and hydrogen peroxide to produce ROS causing apoptosis and cell damage (Benchekroun et al 1993;Chen et al 2013). Therefore, oxidative stress induced in rat liver by ADR was found to be higher than in the controls in our current study.…”

“…*p \ 0.05 versus ADR. All data were expressed as mean ± SEM mitochondria electron transport system (Chen et al 2013) from mainly cytochrome-P450 reductase enzyme (Benchekroun et al 1993) which is important for the metabolism of both endogenous (e.g. lipids, steroids) and exogenous (e.g.…”

“…Recent researches have revealed some potential therapeutic agents, such as Se (Benedetti et al 2012;Dursun and Taskin 2011;Koukay et al 1990;Taskin and Dursun 2012), vitamin E (Koukay et al 1990), NAC (N-acetylcysteine) (Wang et al 2014), a-tocopherol (Chautan et al 1992;Myers et al 1982), quercetin (Chen et al 2013) and L-carnosine (Ozdogan et al 2011) effective in limiting the extent of ADR-induced toxicities, all of which are based on the antioxidant properties of the candidate compound. We have already suggested the selenium to be a possible candidate to eliminate its mitochondrial toxicity in heart and kidney (Taskin and Dursun 2012) tissues.…”