Effect of quercetin glucosides from Allium extracts on HepG2, PC‑3 and HT‑29 cancer cell lines

Pan, Yingkun; Zheng, Yunquan; Ho, Wingkin

doi:10.3892/ol.2018.7893

Cited by 42 publications

(45 citation statements)

References 23 publications

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“…Despite the differences in the volatile sulfur composition, many components are highly similar for both species, as expected, since they are genetically closely related [38]. The volatile sulfur compounds identified in current study are known to be responsible for typical flavor and odor of onions [27,39], as well as for their strong biological activity [17,[40][41][42][43][44]. Some of the relevant Allium species with their volatile sulfur compounds are described in the following section (Data S2).…”

Section: Nosupporting

confidence: 61%

“…The antiproliferative activity of Allium species seems to be related with the synergistic effect of various phytochemicals present in their bulbs of leaves, especially flavonoids and sulfur compounds. Two most abundant flavanols in onion species, quercetin 3,4 -diglucoside and quercetin 4 -monoglucoside, have shown the ability to suppress cell proliferation in three cancer cell lines, HepG2, PC-3, and HT-29 [42]. Similarly, the combination of quercetin and kaempferol was able to reduce cell proliferation in human gut (HuTu-80 and Caco-2) and breast cancer cells (PMC42), which is the evidence of the synergistic effect of phytochemicals isolated from different vegetables used in the human diet [58].…”

Section: Antiproliferative Activity Of a Cornutum And A Cepa Extractsmentioning

confidence: 99%

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Comparison of Organosulfur and Amino Acid Composition between Triploid Onion Allium cornutum Clementi ex Visiani, 1842, and Common Onion Allium cepa L., and Evidences for Antiproliferative Activity of Their Extracts

Fredotović

Soldo

Šprung

et al. 2020

Plants

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Species that belong to the genus Allium have been widely used for human food and traditional medicine. Their beneficial health effects, as well as the specific aroma, are associated with their bioactive chemical compounds, such as sulfur compounds and flavonoids. Gas chromatography and mass spectrometry (GC-MS) and reverse-phase high-performance liquid chromatography (reverse-phase HPLC) were used to identify organosulfur and amino acid content of triploid hybrid onion, Allium cornutum Clement ex Visiani, 1842, and common onion, Allium cepa L. Allium extracts were tested for their antiproliferative activity in three human cancer cell lines (HeLa, HCT116, and U2OS). DNA fragmentation and DAPI staining analysis were performed on HeLa cells to evaluate the effect of extracts on DNA damage and cell morphology. The mRNA expression of p53, Bax, and Caspase-3 genes involved in apoptosis were analyzed by real-time PCR. Using GC-MS, 27 compounds were found in two Allium species headspaces. Differences were noted among the main compound abundance in the headspace (although the major thiols and disulfides were qualitatively identic in both Allium species) and dipropyl disulfide, diisopropyl trisulfide, and (Z)-prop-1-enyl propyl trisulfide were predominant sulfides. Identification of amino acids and their quantities were determined by reverse-phase HPLC. Most abundant amino acids in both onions were arginine (Arg) and glutamic acid (Glu). The results of cytotoxicity testing confirmed antiproliferative effects of both species. The DNA fragmentation assay, DAPI staining and real time PCR analysis confirmed that A. cornutum and A. cepa extracts induced apoptosis in HeLa cells. This study presents the evidence for possible therapeutic use of A. cornutum and A. cepa extracts against human cervical carcinoma cell line.

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Section: Nosupporting

confidence: 61%

Section: Antiproliferative Activity Of a Cornutum And A Cepa Extractsmentioning

confidence: 99%

Comparison of Organosulfur and Amino Acid Composition between Triploid Onion Allium cornutum Clementi ex Visiani, 1842, and Common Onion Allium cepa L., and Evidences for Antiproliferative Activity of Their Extracts

Fredotović

Soldo

Šprung

et al. 2020

Plants

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“…The antiproliferative effect of onion in breast cancer cells is mediated by inhibiting 25 phosphatase with the help of natural tetra sulfide compounds that consequently block cell cycle (Cheng et al, ; Khan et al, ; Ying et al, ). Results have shown that although onion had no considerable toxic effects on HepG2, HT29, and PC3 cell lines, but its quercetin glucosides especially 4′Qmg exhibited significant anticancer activities on these cell lines (Pan et al, ). Perhaps it could be claimed that quercetin and fisetin have the most important role in controlling cancer by onions (Nijveldt et al, ).…”

Section: A Cepamentioning

confidence: 99%

“…These nutritive greens, especially garlic, are associated with a reduced risk of prostate cancer (Chhabra et al, 2018;Nicastro, Ross, & Milner, 2015). Assessing the in vitro and in vivo anticancer activities of Allium species showed that high-volume intake of Allium genus like garlic has prophylaxis effects, which reduces the risk of breast, colon, stomach, and prostate cancers (Miraghajani, Rafie, Hajianfar, Larijani, & Azadbakht, 2018;Pan, Zheng, & Ho, 2018). A study by Chen et al among Taiwanese patients showed that consumption of garlic and onion at least once a week reduced the chance of esophageal cancer (Chen et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Allium vegetables for possible future of cancer treatment

Asemani

Zamani

Bayat

et al. 2019

Phytotherapy Research

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Natural resources such as plants are an upright curing option in treating cancers and reducing the side effects of current therapeutic modalities. Allium genus vegetables are of the most interesting herbs in restricting cancers that includes garlic, onions, leeks, chives, and shallots. These plants have been exploited in folk medicine because of their beneficial health effects in improving numerous diseases. The phytochemical analysis of various Allium genus members showed that, to date, 16 species have proved potential anticancer properties due to the accumulation of various sulfur and organic compounds like S‐allyl mercaptocysteine, quercetin, flavonoids, and ajoene. These compounds with various mechanisms such as hindering cell cycle, inhibiting signaling pathways, inducing apoptosis, and antioxidant activity interfere with diverse stages of formation, growth, differentiation, and metastasis of cancer cells. Similar to garlic and onion, other species have exhibited anticancer activities, so that active natural molecules extracted from them might serve as possible anticancer agents. Therefore, evaluating the main ingredients and studying their anticancer mechanisms are of great importance. In this review, we aim to summarize the available data on anticancer mechanisms of 16 species of Allium genus and their major compounds to assist further researches on the treatment and prevention of cancers.

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“…This action reflected the antitumor impact of the extract on the solid tumors. An in vitro study of the Allium extracts cytotoxic effect against cancer cells was performed by Pan et al [40]. They attributed this cytotoxic effect to the presence of high phenolic compounds such as quercetin glucosides.…”

Section: Discussionmentioning

confidence: 99%

Secondary metabolites and Biological activities of Allium porrum L. attack Ehrlich ascites carcinoma in mice

Elhagrasi

Mahmoud

Foda³

et al. 2019

Egypt. J. Chem.

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A LLIUM vegetables have been recorded to be used in many medical remedies for protection and fighting diseases. The aim of the study is to investigate the phenolic compounds of methanol extract of Allium porrum L. aerial parts (MEAP) and introduce the methanolic extract as a candidate for the treatment of cancer in vivo as well as explore its antitumor mechanisms in attacking tumor cells at the molecular level. Isolation of phenolic and flavonoid compounds by column chromatography has been carried out. They were purified by Sephadex LH-20, and the structures were established with the aid of spectroscopic methods (UV, EI/MS, ESI/MS, 1 H-NMR, and 13 C-NMR spectroscopy). A total of 45 female Swiss albino mice were divided into five groups. One group represents the normal control. The four remaining groups were divided as follows: two groups were injected with Ehrlich ascites carcinoma (EAC) cells intraperitoneally and the other two were injected intramuscularly (to form solid tumor), representing the positive nontreated groups. One group from each model was administrated orally the extract at a dose of 300 mg/kg body weight 3 days weekly for 21 days after 24 h from the intraperitoneal or intramuscular injection of EAC cells. The influence of the extract on the apoptotic proteins were tested in EAC cells and in solid tumor of the treated groups compared with the nontreated ones. Eleven phenolic and flavonoid compounds were isolated from the aerial parts of methanol extract of A. porrum L., and characterized as follows: quercetin-3acid, and gallic acid. The gene expressions of caspase-3, Bax, and Bcl2 proteins and DNA fragmentation test were performed in the EAC cells. Hematological profile was conducted on mice's blood in addition to the Immuno-histochemical determination of P53 protein in solid tumors. MEAP has abundant phenolic compounds. It exhibits tumor suppressing mechanisms through the coordination between the apoptic proteins and DNA fragmentation in the EAC or solid cancer cells. MEAP is suggested to be a potent anticancer agent.

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Effect of quercetin glucosides from Allium extracts on HepG2, PC‑3 and HT‑29 cancer cell lines

Abstract: Abstract. The present study investigated the effects of quercetin glucosides, which were isolated from the Chinese onion

Cited by 42 publications

References 23 publications

Comparison of Organosulfur and Amino Acid Composition between Triploid Onion Allium cornutum Clementi ex Visiani, 1842, and Common Onion Allium cepa L., and Evidences for Antiproliferative Activity of Their Extracts

Comparison of Organosulfur and Amino Acid Composition between Triploid Onion Allium cornutum Clementi ex Visiani, 1842, and Common Onion Allium cepa L., and Evidences for Antiproliferative Activity of Their Extracts

Allium vegetables for possible future of cancer treatment

Secondary metabolites and Biological activities of Allium porrum L. attack Ehrlich ascites carcinoma in mice

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