Quercetin- and caffeic acid-functionalized chitosan-capped colloidal silver nanoparticles: one-pot synthesis, characterization, and anticancer and antibacterial activities

Abstract

The presented study comprises the one-pot synthesis and the characterization of quercetin- and caffeic acid-functionalized chitosan-capped colloidal silver nanoparticles (Ch/Q- and Ch/CA-Ag NPs), and their antibacterial and anticancer activities. The formation of Ch/Q- and Ch/CA-Ag NPs has been confirmed by ultraviolet–visible (UV–vis) spectroscopy, Fourier-transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM). The characteristic surface plasmon resonance (SPR) absorption band has been found at 417 and 424 nm for Ch/Q- and Ch/CA-Ag NPs, respectively. The formation of a chitosan shell comprising quercetin and caffeic acid, which surround the colloidal core Ag NPs, was confirmed by UV–vis, and FTIR analyses, and monitored by TEM microscopy. The size of nanoparticles has been determined as 11.2 and 10.3 nm for Ch/Q- and Ch/CA-Ag, respectively. The anticancer activity of Ch/Q- and Ch/CA-Ag NPs has been evaluated against U-118 MG (human glioblastoma) and ARPE-19 (human retinal pigment epithelium) cells. Both NPs showed anticancer activity, but Ch/Q-Ag NPs seemed to be more effective on cancer cell lines (U-118 MG) in comparison to healthy ones (ARPE-19). Furthermore, the antibacterial activity of Ch/Q- and Ch/CA-Ag NPs against Gram-negative (P. aeruginosa and E. coli) and Gram-positive (S. aureus and S. epidermidis) bacteria was determined, and dose-dependent antibacterial effects were found.

Preparation of caffeic acid grafted chitosan self-assembled micelles to enhance oral bioavailability and antibacterial activity of quercetin

“…This disruption ultimately leads to the demise of the bacterial cells. ( 30 , 31 ). The application of CA in derivative design for the synthesis of novel physiologically active green compounds holds considerable practical significance.…”

Caffeic acid, a dietary polyphenol pre-sensitizes PDAC to chemotherapeutic drug

“…Polyphenols are a vast group of metabolites and are therefore further classi ed into four sub-groups i.e; avonoids, phenolic acids, lignans and stilbenes [14]. Caffeic acid (CFA) [IUPAC name: 3-(3,4dihydroxyphenyl) prop-2-enoic acid] is a phytochemical belonging to the phenolic acids group of polyphenols, it has very promising anti-oxidant activity owing to its catechol group; CFA naturally occurs in green tea, coffee, olive oil, fresh olives, white wine, fruits and vegetables [15]. Along with anti-oxidant properties CFA is reported to have anti-bacterial, anti-viral, anti-obesity, anti-cancer and anti-diabetic effects and is also effective in curing metabolic syndrome and brain-related diseases [16].…”