Quercetin Affects Erythropoiesis and Heart Mitochondrial Function in Mice

Ruíz, Lina; Salazar, Celia; Jensen, Erik L.; Ruiz, Paula A.; Tiznado, William; Quintanilla, Rodrigo A.; Barreto, Marlen; Elorza, Álvaro A.

doi:10.1155/2015/836301

Cited by 24 publications

(20 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We previously reported that quercetin treatment also affected erythropoiesis. Immature erythroid populations showed a significant increase in the number of cells, while the iron-dependent cell populations of erythropoiesis for heme and hemoglobin biosynthesis significantly decreased in quercetin-treated mice [25].…”

Section: Discussionmentioning

confidence: 93%

“…Male C57BL/6 mice were housed in groups of nine mice per cage and maintained at 22 • C on a 12:12-h light-dark cycle, with food and water ad libitum before the procedures. Moreover, male C57BL/6 mice (12 months of age) were daily injected intraperitoneally (i.p) with either 50 mg/kg quercetin (Sigma-Aldrich, Cat # Q4951, Merck KGaA, Darmstadt, Germany) (n = 9), or vehicle (5% DMSO) plus PBS (n = 9) for 15 days, according to the protocol previously described [25].…”

Section: Animalsmentioning

confidence: 99%

See 1 more Smart Citation

Biosystem Analysis of the Hypoxia Inducible Domain Family Member 2A: Implications in Cancer Biology

Salazar

Yáñez

Elorza

et al. 2020

Genes

Self Cite

View full text Add to dashboard Cite

The expression of HIGD2A is dependent on oxygen levels, glucose concentration, and cell cycle progression. This gene encodes for protein HIG2A, found in mitochondria and the nucleus, promoting cell survival in hypoxic conditions. The genomic location of HIGD2A is in chromosome 5q35.2, where several chromosomal abnormalities are related to numerous cancers. The analysis of high definition expression profiles of HIGD2A suggests a role for HIG2A in cancer biology. Accordingly, the research objective was to perform a molecular biosystem analysis of HIGD2A aiming to discover HIG2A implications in cancer biology. For this purpose, public databases such as SWISS-MODEL protein structure homology-modelling server, Catalogue of Somatic Mutations in Cancer (COSMIC), Gene Expression Omnibus (GEO), MethHC: a database of DNA methylation and gene expression in human cancer, and microRNA-target interactions database (miRTarBase) were accessed. We also evaluated, by using Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR), the expression of Higd2a gene in healthy bone marrow-liver-spleen tissues of mice after quercetin (50 mg/kg) treatment. Thus, among the structural features of HIG2A protein that may participate in HIG2A translocation to the nucleus are an importin α-dependent nuclear localization signal (NLS), a motif of DNA binding residues and a probable SUMOylating residue. HIGD2A gene is not implicated in cancer via mutation. In addition, DNA methylation and mRNA expression of HIGD2A gene present significant alterations in several cancers; HIGD2A gene showed significant higher expression in Diffuse Large B-cell Lymphoma (DLBCL). Hypoxic tissues characterize the “bone marrow-liver-spleen” DLBCL type. The relative quantification, by using qRT-PCR, showed that Higd2a expression is higher in bone marrow than in the liver or spleen. In addition, it was observed that quercetin modulated the expression of Higd2a gene in mice. As an assembly factor of mitochondrial respirasomes, HIG2A might be unexpectedly involved in the change of cellular energetics happening in cancer. As a result, it is worth continuing to explore the role of HIGD2A in cancer biology.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Animalsmentioning

confidence: 99%

Biosystem Analysis of the Hypoxia Inducible Domain Family Member 2A: Implications in Cancer Biology

Salazar

Yáñez

Elorza

et al. 2020

Genes

Self Cite

View full text Add to dashboard Cite

show abstract

“…Food intake during quercetin treatment was not significantly reduced in mice on high-fat diets [26] or overweight humans [27]. However, mice receiving injections of quercetin (50 mg/kg) over 15 weeks showed significant body weight loss [28]. Henagan et al [29] reporting on B6 mice on a high-fat diet fed a high dose of quercetin (600 µg/day) observed greater fat gains compared to the controls, whereas a low dose (50 µg/day) significantly reduced fat.…”

Section: Discussionmentioning

confidence: 94%

Quercetin Glycosides Improve Motor Performance and Muscle Weight in Adult Mice

Kanzaki¹,

Takemoto²,

Ono³

et al. 2019

J Nutr Food Sci

View full text Add to dashboard Cite

Male C57BL/6J (B6) mice aged 24 weeks were provided two concentrations (1.5 or 3.0 g/L) of quercetin glycosides (QG) in drinking water over the subsequent 24 weeks. The objective was to determine if QG could improve motor function during early stages of aging. No sustained significant differences in body composition or food intake were noted to confirm the safety of QG treatment. Treated mice had significantly improved performance in grip strength and rotarod tests but not in treadmill performance. The functional benefits were reflected in muscle morphology with increased wet weights of a selected group of muscles (quadratus femoris, gastrocnemius, tibialis anterior, and soleus) in treated mice. Thus, it would appear that long-term oral treatment with QG can safely and effectively improve selected elements of motor performance and increase muscle mass during the early stages of aging.

show abstract

“…Mfn2 (Ruiz et al, 2015). In isolated rat liver mitochondria, quercetin interacted with the mitochondrial membrane and significantly inhibited respiration (Dorta et al, 2008(Dorta et al, , 2005.…”

Section: Discussionmentioning

confidence: 97%

Astaxanthin but not quercetin preserves mitochondrial integrity and function, ameliorates oxidative stress, and reduces heat‐induced skeletal muscle injury

Dohl

Chen

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

Heat stress causes mitochondrial dysfunction and increases mitochondrial production of reactive oxygen species (ROS), both of which contribute to heat‐induced skeletal muscle injury. In this study, we tested whether either astaxanthin or quercetin, two dietary antioxidants, could ameliorate heat‐induced skeletal muscle oxidative injury. In mouse C2C12 myoblasts exposed to 43°C heat stress, astaxanthin inhibited heat‐induced ROS production in a concentration‐dependent manner (1–20 μM), whereas the ROS levels remained high in cells treated with quercetin over a range of concentrations (2–100 µM). Because mitochondria are both the main source and a primary target of heat‐induced ROS, we then tested the effects of astaxanthin and quercetin on mitochondrial integrity and function, under both normal temperature (37°C) and heat stress conditions. Quercetin treatment at 37°C induced mitochondrial fragmentation and decreased membrane potential (ΔΨ m), accompanied by reduced protein expression of the master regulator of mitochondrial biogenesis peroxisome proliferator‐activated receptor‐γ coactivator‐1α (PGC‐1α). It also induced cleavage of mitochondrial inner‐membrane fusion protein OPA1. In contrast, astaxanthin at 37°C increased protein expression of PGC‐1α and mitochondrial transcription factor A (TFAM), and maintained tubular structure and normal ΔΨm. Under 43°C heat stress conditions, whereas quercetin failed to rescue C2C12 cells from injury, astaxanthin treatment prevented heat‐induced mitochondrial fragmentation and depolarization, and apoptotic cell death. We also isolated rat flexor digitorum brevis myofibers and confirmed the data from C2C12 myoblasts that astaxanthin but not quercetin preserves mitochondrial integrity and function and ameliorates heat‐induced skeletal muscle injury. These results confirm that mitochondria may be a potential therapeutic target for heat‐related illness and suggest that astaxanthin may potentially be an effective preventive strategy.

show abstract

Quercetin Affects Erythropoiesis and Heart Mitochondrial Function in Mice

Cited by 24 publications

References 73 publications

Biosystem Analysis of the Hypoxia Inducible Domain Family Member 2A: Implications in Cancer Biology

Biosystem Analysis of the Hypoxia Inducible Domain Family Member 2A: Implications in Cancer Biology

Quercetin Glycosides Improve Motor Performance and Muscle Weight in Adult Mice

Astaxanthin but not quercetin preserves mitochondrial integrity and function, ameliorates oxidative stress, and reduces heat‐induced skeletal muscle injury

Contact Info

Product

Resources

About