Quercetin, a natural product supplement, impairs mitochondrial bioenergetics and locomotor behavior in larval zebrafish (Danio rerio)

Zhang, Jiliang; Souders, Christopher L.; Denslow, Nancy D.; Martyniuk, Christopher J.

doi:10.1016/j.taap.2017.04.024

Cited by 54 publications

(23 citation statements)

References 45 publications

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“…For the behavioral studies, zebrafish were kept for 14 days to accommodate in laboratory conditions and then were divided into 5 groups: the control, the 6-hydroxydopamine (6-OHDA, 250 µM, Sigma-Aldrich, Darmstadt, Germany), and three CsAE treatment groups (0.1, 0.3, and 1 mg/L) administered in three consecutive steps, from 0 to 7 days. The doses of 6-OHDA and CsAE were chosen according to previous reports [52,53]. CsAE (0.1, 0.3, and 1 mg/L) was individually delivered to fish through transferring into 500 mL glass for 1 h, once daily, whereas 6-OHDA (250 µM) treatment was once administered independently by moving into a 500 mL glass, one day before NTT test.…”

Section: Animalsmentioning

confidence: 99%

The Aqueous Extract from Ceratonia siliqua Leaves Protects against 6-Hydroxydopamine in Zebrafish: Understanding the Underlying Mechanism

et al. 2020

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Ceratonia siliqua L. is a Mediterranean medicinal plant traditionally cultivated for its ethnopharmacological benefits, such as antidiarrheal, antidiabetic, enhance acetylcholine, antioxidant, antiatherosclerotic, and for its possible anti-neurodegenerative potential. The aim of the present study was to evaluate the chemical composition, as well as the cognitive-enhancing, anxiolytic, and antioxidant activities of the aqueous extract from C. siliqua (CsAE) leaves against 6-hydroxydopamine (6-OHDA) zebrafish Parkinson’s disease (PD) model. CsAE (0.1, 0.3, and 1 mg/L) was administered by immersion to zebrafish (Danio rerio) for eight consecutive days and one hour before each behavioral test of each day, while 6-OHDA (250 µM) treatment was supplied one day before the novel tank diving test (NTT). Qualitative and quantitative analyses were performed by the ultra-high-performance liquid chromatography (UHPLC) analysis. The memory performance was evaluated through the NTT and Y-maze tests. Additionally, the in vitro and in vivo antioxidant status and acetylcholinesterase (AChE) activity was also assessed. Our finds demonstrated that CsAE presented positive antioxidant and anti-AChE activities, which contributed to the improvement of cognitive function in the 6-OHDA zebrafish PD model.

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Section: Animalsmentioning

confidence: 99%

The Aqueous Extract from Ceratonia siliqua Leaves Protects against 6-Hydroxydopamine in Zebrafish: Understanding the Underlying Mechanism

et al. 2020

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“…The reason behind the curcumin toxicity was previously reported as; it induced caspase-3 dependent apoptosis in Human Jurkat cells. 39) Gallic acid shows PE as well as delayed hatching (Dh) in treatment at 200 µg/mL whereas quercetin showed pericardial abnormalities at higher concentration (500 µg/mL).…”

Section: Morphological Deformationmentioning

confidence: 99%

“…Thus, larval behaviour was affected to cause locomotion. 40) In our study, the acute toxicity of these compounds for this model has been previously evaluated. It also helps in preventing angiogenesis by inhibiting VEGFR2 mediated network.…”

Section: Morphological Deformationmentioning

confidence: 99%

Toxicity and Selective Biochemical Assessment of Quercetin, Gallic Acid, and Curcumin in Zebrafish

Harishkumar

Reddy

Karadkar

et al. 2019

Biological & Pharmaceutical Bulletin

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In recent years, numerous research outcomes were established on various naturally occurring compounds that have been shown to have beneficial antioxidant and other biological activities. Antioxidant defence mechanism plays a vital role in combating various diseases mainly due to oxidative stress. However, various models have been utilized to identify their bioactivities using these compounds (quercetin, gallic acid and curcumin). Their toxicity level also has to be explored to determine the threshold levels on the usage of these compounds. In this study, we investigated the lethal concentration of these compounds and abnormalities, biochemical and morphological changes in zebrafish embryo (Danio rerio). Toxicity level was evaluated by calculating the LD 50 on the embryonic stages at 24, 48 and 72 h. Antioxidant parameters such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and biological assays such as lipid peroxidation, protein estimation were performed. Microscopic evaluations were also observed to find out morphological abnormalities. However, these naturally derived compounds are reported to have their protective and curative role in many health complications. From the above assays, we are studying the effect of the drugs in both biochemical and molecular way in the zebrafish model organism.

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“…Previous studies have suggested that chemical-induced alterations in mitochondrial bioenergetics in zebrafish embryos may contribute to developmental toxicity (e.g. curved tails or abnormal hatching time) (Wang et al, 2018;Zhang et al, 2017). Noteworthy is that hatching delay is reported to be associated with the interference in the thyroid hormone (TH) system (Timme-Laragy et al, 2006).…”

Section: Hatching Time Of Zebrafish Embryos Is Affected Differently Bmentioning

confidence: 99%

Biological effects of the benzotriazole ultraviolet stabilizers UV-234 and UV-320 in early-staged zebrafish (Danio rerio)

Liang

Adamovský

Souders

et al. 2019

Environmental Pollution

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Among the benzotriazole ultraviolet stabilizers (BUVSs), UV-234 and UV-320 are frequently detected in aquatic ecosystem. Despite the fact that these chemicals are present in low ng/L levels in surface water, they show high bio-accumulation potential and pose exposure risks to aquatic organisms. However, there are limited toxicological data available in fish. In this study, zebrafish embryos were exposed to 0.01, 0.1 and 1 mM UV-234 or UV-320 for up to 6 days. Developmental toxicity as well as effects on mitochondrial bioenergetics, immune system responses, and locomotor activity in zebrafish were measured. After UV-234 treatment (0.1e1 mM), hatching time of embryos was increased compared to controls. There was also a~20e40% reduction in non-mitochondrial respiration and oligomycindependent mitochondrial respiration in embryos treated with 1 mM UV-234 for 24 and 48 h respectively; conversely basal respiration and non-mitochondrial respiration were increased~20e30% in embryos treated with 1 mM UV-320 at 48 h. Transcript levels of sod1 were down-regulated with BUVSs while sod2 mRNA was highly up-regulated with both UV-234 and UV-320, suggesting an oxidative damage response. Considering that mitochondrial signaling regulates innate immune pathways, we measured the expression of immune related transcripts (tlr5a, tlr5b, mmp9, il8, tnfa, cxcl-C1c, nfkb1, and ifng). Of these, only il8 and cxcl-C1c mRNA were decreased in response to 0.1 mM UV-320. To associate early molecular events with behavior, locomotor activity was assessed. UV-234 reduced larval activity in a dark photokinesis assay by~15%, however behavioral responses at environmentally-relevant concentrations of BUVSs were not consistent across experiments nor BUVSs. These data suggest that BUVSs can perturb mitochondrial bioenergetics, embryonic development, and locomotor activity of zebrafish, but these responses appear to be dose-, time-and BUVSs dependent, suggesting these chemicals may have unique modes of action.

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Quercetin, a natural product supplement, impairs mitochondrial bioenergetics and locomotor behavior in larval zebrafish (Danio rerio)

Cited by 54 publications

References 45 publications

The Aqueous Extract from Ceratonia siliqua Leaves Protects against 6-Hydroxydopamine in Zebrafish: Understanding the Underlying Mechanism

The Aqueous Extract from Ceratonia siliqua Leaves Protects against 6-Hydroxydopamine in Zebrafish: Understanding the Underlying Mechanism

Toxicity and Selective Biochemical Assessment of Quercetin, Gallic Acid, and Curcumin in Zebrafish

Biological effects of the benzotriazole ultraviolet stabilizers UV-234 and UV-320 in early-staged zebrafish (Danio rerio)

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