Toxicity and Selective Biochemical Assessment of Quercetin, Gallic Acid, and Curcumin in Zebrafish

Abstract

In recent years, numerous research outcomes were established on various naturally occurring compounds that have been shown to have beneficial antioxidant and other biological activities. Antioxidant defence mechanism plays a vital role in combating various diseases mainly due to oxidative stress. However, various models have been utilized to identify their bioactivities using these compounds (quercetin, gallic acid and curcumin). Their toxicity level also has to be explored to determine the threshold levels on the usage of these compounds. In this study, we investigated the lethal concentration of these compounds and abnormalities, biochemical and morphological changes in zebrafish embryo (Danio rerio). Toxicity level was evaluated by calculating the LD 50 on the embryonic stages at 24, 48 and 72 h. Antioxidant parameters such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and biological assays such as lipid peroxidation, protein estimation were performed. Microscopic evaluations were also observed to find out morphological abnormalities. However, these naturally derived compounds are reported to have their protective and curative role in many health complications. From the above assays, we are studying the effect of the drugs in both biochemical and molecular way in the zebrafish model organism.

A network pharmacology approach to identify the mechanisms and molecular targets of curcumin against Alzheimer disease

“…Curcumin compliance with Lipinski rule of 5, and with a higher LD50 value. The literature has previously reported intraperitoneal administration of curcumin has shown LD50 in the mouse at 1500 mg/kg, [41] which is close to the predicted result by the electronic databases. Afterward, a total of 49 targets of curcumin against AD targets were obtained.…”

Protective effects of quercetin, polydatin, and folic acid and their mixtures in a zebrafish (Danio rerio) fetal alcohol spectrum disorder model

“…In our previous work (Cadena et al, 2020), FA also reduced small and high activity in the excitatory light-dark locomotion test, as shown here in the light test. Also, QUE had the lowest toxicity when compared with gallic acid and curcumin in zebrafish embryos (Harishkumar et al, 2019), but these authors did not study the behavioral effects. QUE, POL and FA treatment without ethanol did not affect the epiboly or development of the larvae, but embryonic exposure to QUE, POL and FA affected the larval behavior.…”

Herbal Preparation (Bromelain, Papain, Curcuma, Black Pepper) Enhances Mineralization and Reduces Glucocorticoid-Induced Osteoporosis in Zebrafish

“…It is interesting to note how our studies show a lethal concentration of 100 μM, while a previous study defined 7.5 μM as the lethal dose of curcumin only on zebrafish embryo [ 29 ]. Another study on zebrafish embryos identified LD50 as 135 μg/mL (350 μM), but an early mortality window appeared at 30–50 μg/mL (50–150 μM) curcumin [ 30 ].…”