QuEChERS‐based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC‐MS/MS

Flores, María Isabel Alarcón; Romero‐González, Roberto; Vidal, José Luis Martı́nez

doi:10.1002/jssc.201100093

Cited by 43 publications

(24 citation statements)

References 35 publications

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“…Relatively short dwell times (≤ 50 ms) typical for modern QqQ mass spectrometers make a rapid analysis of multiple hormones possible [15]. Application of ultra performance liquid chromatography (UPLC) [16] as well as solid-phase extraction (SPE)-based enrichment [17] may further improve sensitivity of phytohormone analysis. Nevertheless, obtained in the presence of biological matrix method quantification limits (LOQ m ) reported for JA, SA and ABA typically do not exceed 2.5 × 10 -14 mol (even with top-sensitive instruments).…”

Section: Introductionmentioning

confidence: 99%

An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues

et al. 2012

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BackgroundPhytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and modulating plant reactions targeted against pathogens and herbivores, as well as resistance to abiotic stress (drought, UV-irradiation and mechanical wounding). These factors induce dramatic changes in phytohormone biosynthesis and transport leading to rapid local and systemic stress responses. Understanding of underlying mechanisms is of principle interest for scientists working in various areas of plant biology. However, highly sensitive, precise and high-throughput methods for quantification of these phytohormones in small samples of plant tissues are still missing.ResultsHere we present an LC-MS/MS method for fast and highly sensitive determination of jasmonates, abscisic and salicylic acids. A single-step sample preparation procedure based on mixed-mode solid phase extraction was efficiently combined with essential improvements in mobile phase composition yielding higher efficiency of chromatographic separation and MS-sensitivity. This strategy resulted in dramatic increase in overall sensitivity, allowing successful determination of phytohormones in small (less than 50 mg of fresh weight) tissue samples. The method was completely validated in terms of analyte recovery, sensitivity, linearity and precision. Additionally, it was cross-validated with a well-established GC-MS-based procedure and its applicability to a variety of plant species and organs was verified.ConclusionThe method can be applied for the analyses of target phytohormones in small tissue samples obtained from any plant species and/or plant part relying on any commercially available (even less sensitive) tandem mass spectrometry instrumentation.

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Section: Introductionmentioning

confidence: 99%

An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues

et al. 2012

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“…These include LLE combined with gel chromatography purification method, which was developed to analyze 46 kinds of banned synthetic pigments [5]; the QuEChERS method [23][24][25]; and QuEChERS method combined with MCS-SPE, which was developed to analyze ten kinds of plant growth regulator in bean sprouts [3,4]. Other examples of purification method include SLW-SPE and GC-MS, which were previously shown to detect the residues of 26 ␤-agonists and hormones in meat tissues [3]; and LC-MS combined with dispersive SPE, which was used in the detection of eight kinds of banned veterinary drugs.…”

Section: Gradient Purification By Spementioning

confidence: 99%

Determination of three kinds of banned drugs in milk powder by hybrid solid‐phase extraction purification combined with gas chromatography and mass spectrometry

Cai

Yang

et al. 2015

J of Separation Science

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A gradient clean-up method for the quantification of five kinds of banned drugs (two hormones, two sedatives, and one chloramphenicol) in milk powder was developed. We used the combination of solid-phase extraction purification with gas chromatography and mass spectrometry. Milk powder was initially hydrolyzed by β-glucuronidase/arylsulfatase, and then the hydrolyzed solution was concentrated and purified using a C and cation resin solid-phase extraction column. To isolate hormones and chloramphenicol drugs, products from the previous step were diluted with methanol and further purified using a silica and diatomite solid-phase extraction column. After derivatization, the drugs were analyzed by gas chromatography with mass spectrometry, and the hydrolyzed solution was diluted with 5% ammoniated methanol to purify sedatives before gas chromatography with mass spectrometry analysis. Results showed that after adding the banned drugs at concentrations of 0.3-10.0 μg/kg, the average recovery range was 78.2-97.3% with relative standard deviations of 5.3-12.5%. The limit of quantification of the banned drugs (S/N ≥ 10) was 0.3-5.0 μg/kg, whereas the limit of detection (S/N ≥ 3) was 0.1-2.0 μg/kg. The solid-phase extraction gradient purification system was simple, rapid, and accurate, and could satisfy the detection requirements of hormone, sedatives, and chloramphenicol drugs when used together with gas chromatography and mass spectrometry.

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“…It now probably represents one of the world's most employed approaches for sample preparation in pesticide residue analysis. [26][27][28][29][30][31][32] Meanwhile, isotope dilution mass spectrometry has been widely used in food safety analysis because of the high precision, high accuracy, and simple pretreatment for samples.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of 19 Plant Growth Regulator Residues in Plant-originated Foods by QuEChERS and Stable Isotope Dilution-Ultra Performance Liquid Chromatography-Mass Spectrometry

Liu¹,

Fang³

et al. 2017

ANAL. SCI.

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Appropriate and fast analytical methods are required for plantoriginated foods, given the great significance therein for food safety, human health and social economic development. In this study, a highly sensitive and fast method of QuEChERS (acronym of quick, easy, cheap, effective, rugged, safe) combined with isotope dilution-ultra performance liquid chromatography coupled to tandem mass spectrometry was developed for the simultaneous determination of 19 plant growth regulators in plant-originated foods. The samples were initially extracted with acetonitrile containing 1% acetic acid and then the QuEChERS method was applied after the pH value was adjusted to 5.5 -6.0, using 3-indolepropionic-d2 acid and forchlorfenuron-d5 as internal standard. The targeted 19 plant growth regulators were separated on an HSS T3 column using acetonitrile: 5 mmol/L ammonium acetate as the mobile phase. Quantitative results were based on multiple reaction monitoring mode after ionization in positive and negative electrospray ionization mode. Good linearity was achieved within a wide range and all the correlation coefficients were greater than 0.997. The limit of quantification was 0.060 -6.0 μg/kg. Rate of recovery and relative standard deviation were 72.3 -115.8 and 1.78 -5.32%, respectively. The method was successfully applied to measure 19 plant growth regulator residues in 280 plant-originated commercial foods collected from local supermarkets in China. Twelve plant growth regulators were found in some of the analyzed samples.

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QuEChERS‐based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC‐MS/MS

Cited by 43 publications

References 35 publications

An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues

An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues

Determination of three kinds of banned drugs in milk powder by hybrid solid‐phase extraction purification combined with gas chromatography and mass spectrometry

Simultaneous Determination of 19 Plant Growth Regulator Residues in Plant-originated Foods by QuEChERS and Stable Isotope Dilution-Ultra Performance Liquid Chromatography-Mass Spectrometry

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