Quaternary ammonium compound as antimicrobial agent in resin-based sealants

García, Isadora Martini; Rodrigues, Stéfani Becker; Balbinot, Gabriela de Souza; Visioli, Fernanda; Leitune, Vicente Castelo Branco; Collares, Fabrício Mezzomo

doi:10.1007/s00784-019-02971-4

Cited by 24 publications

(35 citation statements)

References 34 publications

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“…The high prevalence of failed restorations has made secondary caries a pernicious problem [ 1 ]. The onset of primary or recurrent carious lesions is triggered by the biofilm growth over the tooth surface or tooth–material interface [ 2 , 3 ]. Caries-related biofilms are composed of a densely filled community of microbial cells.…”

Section: Introductionmentioning

confidence: 99%

Light Energy Dose and Photosensitizer Concentration Are Determinants of Effective Photo-Killing against Caries-Related Biofilms

Balhaddad

AlQranei

Ibrahim

et al. 2020

IJMS

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Caries-related biofilms and associated complications are significant threats in dentistry, especially when biofilms grow over dental restorations. The inhibition of cariogenic biofilm associated with the onset of carious lesions is crucial for preventing disease recurrence after treatment. This in vitro study defined optimized parameters for using a photosensitizer, toluidine blue O (TBO), activated via a red light-emitting diode (LED)-based wireless device to control the growth of cariogenic biofilms. The effect of TBO concentrations (50, 100, 150, and 200 μg/mL) exposed to light or incubated in the dark was investigated in successive cytotoxicity assays. Then, a mature Streptococcus mutans biofilm model under sucrose challenge was treated with different TBO concentrations (50, 100, and 150 μg/mL), different light energy doses (36, 108, and 180 J/cm2), and different incubation times before irradiation (1, 3, and 5 min). The untreated biofilm, irradiation with no TBO, and TBO incubation with no activation represented the controls. After treatments, biofilms were analyzed via S. mutans colony-forming units (CFUs) and live/dead assay. The percentage of cell viability was within the normal range compared to the control when 50 and 100 μg/mL of TBO were used. Increasing the TBO concentration and energy dose was associated with biofilm inhibition (p < 0.001), while increasing incubation time did not contribute to bacterial elimination (p > 0.05). Irradiating the S. mutans biofilm via 100 μg/mL of TBO and ≈180 J/cm2 energy dose resulted in ≈3-log reduction and a higher amount of dead/compromised S. mutans colonies in live/dead assay compared to the control (p < 0.001). The light energy dose and TBO concentration optimized the bacterial elimination of S. mutans biofilms. These results provide a perspective on the determining parameters for highly effective photo-killing of caries-related biofilms and display the limitations imposed by the toxicity of the antibacterial photodynamic therapy’s chemical components. Future studies should support investigations on new approaches to improve or overcome the constraints of opportunities offered by photodynamic inactivation of caries-related biofilms.

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Section: Introductionmentioning

confidence: 99%

Light Energy Dose and Photosensitizer Concentration Are Determinants of Effective Photo-Killing against Caries-Related Biofilms

Balhaddad

AlQranei

Ibrahim

et al. 2020

IJMS

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“…For the assessment of antibacterial property, discs of the polymerized dental resins entered into contact with an enriched broth containing S. mutans as previously performed [13,33,[40][41][42].…”

Section: Discussionmentioning

confidence: 99%

“…To evaluate the possible cytotoxic effect of the experimental dental resin, human keratinocytes (HaCaT, CLS Cell Lines Service GmbH, Eppelheim, Germany) were used in this test [40]. All reagents used to evaluate the possible cytotoxicity of the dental resins were purchased from Aldrich Chemical Company (St. Louis, MO, USA).…”

Section: Cytotoxicitymentioning

confidence: 99%

Myristyltrimethylammonium Bromide (MYTAB) as a Cationic Surface Agent to Inhibit Streptococcus mutans Grown over Dental Resins: An In Vitro Study

Nunes

et al. 2020

JFB

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This in vitro study evaluated the effect of myristyltrimethylammonium bromide (MYTAB) on the physical, chemical, and biological properties of an experimental dental resin. The resin was formulated with dental dimetacrylate monomers and a photoinitiator/co-initiator system. MYTAB was added at 0.5 (G 0.5% ), 1 (G 1% ), and 2 (G 2% ) wt %, and one group remained without MYTAB and was used as the control (G Ctrl ). The resins were analyzed for the polymerization kinetics, degree of conversion, ultimate tensile strength (UTS), antibacterial activity against Streptococcus mutans, and cytotoxicity against human keratinocytes. Changes in the polymerization kinetics profiling were observed, and the degree of conversion ranged from 57.36% (±2.50%) for G 2% to 61.88% (±1.91%) for G 0.5% , without a statistically significant difference among groups (p > 0.05). The UTS values ranged from 32.85 (±6.08) MPa for G 0.5% to 35.12 (±5.74) MPa for G Ctrl (p > 0.05). MYTAB groups showed antibacterial activity against biofilm formation from 0.5 wt % (p < 0.05) and against planktonic bacteria from 1 wt % (p < 0.05). The higher the MYTAB concentration, the higher the cytotoxic effect, without differences between G Ctrl e G 0.5% (p > 0.05). In conclusion, the addition of 0.5 wt % of MYTAB did not alter the physical and chemical properties of the dental resin and provided antibacterial activity without cytotoxic effect.

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“…The antibacterial sealant formulation demonstrated comparable mechanical properties and higher ultimate tensile strength compared to the control [ 81 ]. The use of [2-(methacryloyloxy)ethyl] trimethylammonium chloride (METAC) antibacterial monomer in the sealant formulation was also associated with a 1-log reduction of S. mutans biofilms [ 82 ], which could be effective in minimizing biofilm formation in teeth with deep pits and fissures.…”

Section: Contact-killing Materials As a Strategy In Resin-based Rementioning

confidence: 99%

Emerging Contact-Killing Antibacterial Strategies for Developing Anti-Biofilm Dental Polymeric Restorative Materials

et al. 2020

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Polymeric materials are the first choice for restoring tooth cavities, bonding tooth-colored fillings, sealing root canal systems, and many other dental restorative applications. However, polymeric materials are highly susceptible to bacterial attachment and colonization, leading to dental diseases. Many approaches have been investigated to minimize the formation of biofilms over polymeric restorative materials and at the tooth/material interfaces. Among them, contact-killing compounds have shown promising results to inhibit dental biofilms. Contact-killing compounds can be immobilized within the polymer structure, delivering a long-lasting effect with no leaching or release, thus providing advantages compared to release-based materials. This review discusses cutting-edge research on the development of contact-killing compounds in dental restorative materials to target oral pathogens. Contact-killing compounds in resin composite restorations, dental adhesives, root canal sealers, denture-based materials, and crown cements have all demonstrated promising antibacterial properties. Contact-killing restorative materials have been found to effectively inhibit the growth and activities of several oral pathogens related to dental caries, periodontal diseases, endodontic, and fungal infections. Further laboratory optimization and clinical trials using translational models are needed to confirm the clinical applicability of this new generation of contact-killing dental restorative materials.

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Quaternary ammonium compound as antimicrobial agent in resin-based sealants

Cited by 24 publications

References 34 publications

Light Energy Dose and Photosensitizer Concentration Are Determinants of Effective Photo-Killing against Caries-Related Biofilms

Light Energy Dose and Photosensitizer Concentration Are Determinants of Effective Photo-Killing against Caries-Related Biofilms

Myristyltrimethylammonium Bromide (MYTAB) as a Cationic Surface Agent to Inhibit Streptococcus mutans Grown over Dental Resins: An In Vitro Study

Emerging Contact-Killing Antibacterial Strategies for Developing Anti-Biofilm Dental Polymeric Restorative Materials

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