Quartz tuning fork as a mass sensitive biosensor platform with a bi-layer film modification via plasma polymerization

Özgüzar, Hatice Ferda; Can, Gizem Kaleli; Kabay, Gözde; Mutlu, Mehmet

doi:10.1557/mrc.2019.55

Cited by 9 publications

(6 citation statements)

References 37 publications

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“…To avoid contamination, the chamber was washed (100 W discharge power for 15 min) with the same precursor (O 2 or amine-rich precursors) to be utilized at the following modification steps. [31][32][33] First, surface activation of bare polypropylene (b-PPs) substrates was carried out by O 2 plasma-assisted etching at 100 W discharge power for 15 min. [31] In the second step, the O 2 -activated PP substrate (O 2 PPs) surfaces were exposed to ethylenediamine (EDA) and ammonia (NH 3 ) precursor mixture [EDA:NH 3 , 1:1 (v:v)] mediated glow discharge.…”

Section: Sample Preparation and Plasma Modificationmentioning

confidence: 99%

Plasma‐Assisted Surface Modification and Heparin Immobilization: Dual‐Functionalized Blood‐Contacting Biomaterials with Improved Hemocompatibility and Antibacterial Features

Özgüzar

Evren

Meydan

et al. 2022

Adv Materials Inter

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The inferior hemocompatibility or antibacterial properties of blood‐contacting materials and devices are restraining factors that hinder their successful clinical utilization. To highlight these, a plasma‐enhanced modification strategy is favored for surface tailoring of an extensively used biomaterial, polypropylene (PP). The surface activation of the PPs is achieved by oxygen plasma etching and subsequent surface functionalization through amine‐rich precursor mediated coating by plasma glow discharge. After optimum plasma processing parameters are decided, heparin (anticoagulant and antithrombic drug) is either attached or covalently conjugated on the PPs’ surfaces. The aminated films produced at 75 W plasma power with 15 min exposure time are highly hydrophilic (34.72 ± 5.92°) and surface active (65.91 mJ m−2), facilitating high capacity heparin immobilization (≈440 µg cm−2) by covalent linkage. The kinetic‐blood coagulation rate and protein adhesion amount on the plasma‐mediated heparinized PPs are decreased about tenfold and 15‐fold, and platelet adhesion is markedly lowered. In addition, heparinized‐PP surfaces comprise superior antibacterial activity against gram‐positive/‐negative bacteria conveyed particularly by contact‐killing (99%). The heparin‐coating did not cause cytotoxicity on fibroblast cells, instead enhanced their proliferation, as shown by the (3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐Diphenyltetrazolium Bromide) assay. Overall, this simple methodology is highly proficient in becoming a universal strategy for developing dual‐functionalized blood‐contacting materials.

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Section: Sample Preparation and Plasma Modificationmentioning

confidence: 99%

Plasma‐Assisted Surface Modification and Heparin Immobilization: Dual‐Functionalized Blood‐Contacting Biomaterials with Improved Hemocompatibility and Antibacterial Features

Özgüzar

Evren

Meydan

et al. 2022

Adv Materials Inter

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“…The equation is modified for the frequency measurement in liquid phase due to bulk liquid properties like conductivity, viscosity, density and dielectric constant. In the liquid phase, the frequency shifts are determined by the Bruckenstein-Shay and Kanazawa-Gordon's equation as follows [31][32]:…”

Section: Detection Scheme Of Quartz Crystal Microbalancementioning

confidence: 99%

“…In the literature, plasma modified mass sensitive biosensors used in biomedical application have been presented for detecting genetically modified organism, human pathogen and mostly model protein, bovine serum albumin (BSA) over the past decade [14,19,[29][30][31][32]. A DNA biosensor, which uses single-stranded DNA probe to detect human pathogen, Vibrio parahaemolyticus, based on a quartz crystal microbalance has been used.…”

Section: Applications Of Plasma Modified Qcm and Qtf In Biomedical Applicationsmentioning

confidence: 99%

“…At last, a QTF-based platform was produced by using a bi-layer film that coated by plasma-polymerized n-heptane (hep) and then by ethylenediamine (EDA), respectively in our laboratory. Then, the BSA detection performance of mass sensitive biosensor was tested and 20±3.60 Hz [32].…”

Section: Applications Of Plasma Modified Qcm and Qtf In Biomedical Applicationsmentioning

confidence: 99%

“…Nowadays, plasma technologies have been started to be used for the modification of the surface of the quartz crystal [14,19,[25][26][27][28][29][30][31][32]. This technique give chance to cover the crystal surfaces with control film growth down to the angstrom (Å) scale with uniform coating, while preserving the bulk properties of the substrate material.…”

Section: Introductionmentioning

confidence: 99%

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Plasma Polymerized Films for Mass Sensitive Biosensors

Can

Mutlu

2019

Natural and Applied Sciences Journal

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Mass sensitive biosensors represent promising tool that used in many areas such as biomedical applications, food, environmental, military and in other fields instead of conventional methods. However, surface modifications are needed to design this rapid and reliable sensors. Plasma polymerization is a commonly used technology which offers easily-controllable, environmentally friendly, and inexpensive processing of various materials when compared to the wet chemical methods. This review includes working principle of mass sensitive biosensors, surface modification of piezoelectric crystals by plasma technology and applications of these crystals as a mass sensitive biosensor in biomedical applications.

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Development of QTF-based mass-sensitive immunosensor for phenylketonuria diagnosis

2022

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Quartz tuning fork as a mass sensitive biosensor platform with a bi-layer film modification via plasma polymerization

Abstract: Abstract

Cited by 9 publications

References 37 publications

Plasma‐Assisted Surface Modification and Heparin Immobilization: Dual‐Functionalized Blood‐Contacting Biomaterials with Improved Hemocompatibility and Antibacterial Features

Plasma‐Assisted Surface Modification and Heparin Immobilization: Dual‐Functionalized Blood‐Contacting Biomaterials with Improved Hemocompatibility and Antibacterial Features

Plasma Polymerized Films for Mass Sensitive Biosensors

Development of QTF-based mass-sensitive immunosensor for phenylketonuria diagnosis

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