Quartz Crystal Microbalance In-Line Sensing of Escherichia Coli in a Bioreactor Using Molecularly Imprinted Polymers

Samardzic, Renata; Sussitz, Hermann F.; Jongkon, Nathjanan; Lieberzeit, Peter A.

doi:10.1166/sl.2014.3201

Cited by 20 publications

(15 citation statements)

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“…Overall, the QCM sensors have shown similar sensitivity to SPR, afforded 10 regeneration cycles and worked for at least 3 months[104]. In an example of practical application, PU-based QCM sensor was used to follow growth processes of Escherichia coli and Saccharomyces cerevisiae in a bioreactor[105,106]. The sensor was able to identify different stages of the cell cycle, with a LoD of 1.6 × 10 8 cells/mL.…”

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confidence: 99%

Molecularly Imprinted Polymers for Cell Recognition

Piletsky

Canfarotta²,

Poma

et al. 2020

Trends in Biotechnology

194

116

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Since their conception fifty years ago, molecularly imprinted polymers (MIPs) have seen extensive development both in terms of synthetic routes and applications. Perhaps the most challenging target for molecular imprinting are cells. Though early work was based almost entirely around microprinting methods, recent developments shifted towards epitope imprinting to generate MIP nanoparticles. Simultaneously, the development of techniques such as solid phase MIP synthesis have solved many historic issues of MIP production. This review briefly describes various approached used in cell imprinting with a focus on applications of the created materials in imaging, drug delivery, diagnostics and tissue engineering.

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mentioning

confidence: 99%

Molecularly Imprinted Polymers for Cell Recognition

Piletsky

Canfarotta²,

Poma

et al. 2020

Trends in Biotechnology

194

116

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“…Furthermore, previous experience with chemically related MIP matrices shows that they are much more rugged than biospecies. A MIP thin film selectively binding E. coli could be operated in a bioreactor for a month [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Combined Layer/Particle Approaches in Surface Molecular Imprinting of Proteins: Signal Enhancement and Competition

Phan

Sussitz

Ladenhauf

et al. 2018

Sensors

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Herein we report novel approaches to the molecular imprinting of proteins utilizing templates sizing around 10 nm and some 100 nm. The first step comprised synthesizing nanoparticles of molecularly imprinted polymers (MIP) towards bovine serum albumin (BSA) and characterizing them according to size and binding capacity. In a second step, they were utilized as templates. Quartz crystal microbalances (QCM) coated with MIP thin films based on BSA MIP nanoparticles lead to a two-fold increase in sensor responses, compared with the case of directly using the protein as the template. This also established that individual BSA molecules exhibit different “epitopes” for molecular imprinting on their outer surfaces. In light of this knowledge, a possible MIP-based biomimetic assay format was tested by exposing QCM coated with BSA MIP thin films to mixtures of BSA and imprinted and non-imprinted polymer (NIP) nanoparticles. At high protein concentrations (1000 ppm) measurements revealed aggregation behavior, i.e., BSA binding MIP NP onto the MIP surface. This increased sensor responses by more than 30% during proof of concept measurements. At lower a BSA concentration (500 ppm), thin films and particles revealed competitive behavior.

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“…Many possible applications for QCM systems have been described in the fields of food [67], environment [68], and clinic analysis [69], etc. Samardzic et al [70] developed a sensor based on a MIP coated QCM as the mass sensitive transducer. They were able to reach a limit of detection of 1.6* Â 10 8 cells/mL within a few minutes.…”

Section: Piezoelectric Biosensormentioning

confidence: 99%

Molecular imprinting technology for microorganism analysis

Jia

Zhong

et al. 2018

TrAC Trends in Analytical Chemistry

125

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Molecular imprinting technology has been widely applied to various fields, owing to unique features of structure predictability, recognition specificity and application universality. Microorganism imprinting has attracted significant interests attributing to the high selectivity, simplicity rapidity, and excellent stability as well as low cost and eco-friendliness. Herein, we purpose to review the recent advances of MIT for microorganism analysis, concerning imprinting methods, analytical detection methods and typical applications. Various imprinting methods including direct and indirect imprinting for microorganism-MIPs preparation are comprehensively summarized. MIPs based biosensors containing fluorescence, electrochemical, piezoelectric and surface plasmon resonance for analytical detection of microorganisms is highlighted. Representative applications of microbiological imprinting are discussed, involving detection and quantification of bacteria, identification of bacterial species, and determination of yeast growth status. Finally, we propose the remaining challenges and future perspectives to accelerate the development and utilization of MIT in microorganism analysis and thereby push forwards microorganism identification and determination.

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Quartz Crystal Microbalance In-Line Sensing of Escherichia Coli in a Bioreactor Using Molecularly Imprinted Polymers

Cited by 20 publications

References 0 publications

Molecularly Imprinted Polymers for Cell Recognition

Molecularly Imprinted Polymers for Cell Recognition

Combined Layer/Particle Approaches in Surface Molecular Imprinting of Proteins: Signal Enhancement and Competition

Molecular imprinting technology for microorganism analysis

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