Quantum dot bio-conjugate: as a western blot probe for highly sensitive detection of cellular proteins

Functionalized Quantum Dots for Biosensing and Bioimaging and Concerns on Toxicity

“…For example, competitive fluoroimmunoassay based on QDantibody conjugates has been developed for detection of different targets ranging from small molecules to proteins, virus and bacteria. 6,7 In addition to the analyzing abilities of QDs, recent advances in QD-based immunoassay with ultrahigh sensitivity or rapid screening capability have also benefited the developments of novel readout methods, such as electrochemical detection, barcode, microfluidics technique and other readout strategies. 8,9 For example, ultrasensitive western blot analysis based on QDmonoclonal antibody conjugates was carried out with electrophoresis to screen protein expression in cell or tissue with ultrahigh specificity and throughput.…”

“…Developments in QD surface engineering have enabled them to be used as novel fluorescent probes for sensing and analyzing a wide variety of chemical and biological analysts. For example, competitive fluoroimmunoassay based on QD-antibody conjugates has been developed for detection of different targets ranging from small molecules to proteins, virus and bacteria. , …”

Synthesis and characterization of CdTe QDs capped with a branched 3MB3MP ligand and fluorescent switching detection of H2O2

“…The peaks at 125 and 142 cm À1 correspond to the A1 and E1 mode of Te and the fundamental transverse optical mode of CdTe. 26,27 XPS analysis was carried out to determine the elemental composition and to identify the chemical states of the elements present in the QDs. Fig.…”

Microfluidic synthesis of quantum dots and their applications in bio-sensing and bio-imaging

“…Advantages of high sensitivity were also shown in the detection of other proteins like biomarkers for Alzheimer's disease 90 or the intracellular cytoplasmic protein β-actin. 91 Ricardo et al 92 reported a microfluidic method allowing control of the coupling between QDs and proteins. This microfluidic method improved the labelling process, and provided a rapid and efficient method that enabled the traceability of lectins.…”