With the rapid development of laser technology, information technology, and materials, the detection of trace gas has been taken to a new level. Advanced analytical methods were invented, for example, gas chromatography, mass spectrometry, chemical sensors, absorption spectrum, and so forth. With the detailed data gathered from new high-tech equipment, we can predict and diagnose diseases such as diabetic ketoacidosis, irritable bowel syndrome, lung cancer and so on. However, there is still a large amount of exhaled gas that has not been discovered, which means that breath analysis of exhaled gas is a very promising field in the future. Non-invasive breath diagnosis and monitoring technology have attracted huge attention from all over the world. Using exhaled gas to diagnose and monitor human disease has numerous advantages for being non-invasive, convenient and environment friendly.