Quanto a escova deve ser introduzida no canal anal para avaliação citológica mais eficaz?

Nadal, Sidney Roberto; Horta, Sérgio Henrique Couto; Calore, Edenilson Eduardo; Nadal, Luís Roberto Manzione; Manzione, Carmen Rutn

doi:10.1590/s0104-42302009000600022

Cited by 16 publications

(9 citation statements)

References 30 publications

(41 reference statements)

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“…Microarray analysis was carried out using the PAPILLOCHECK® (GREINER BIO‐ONE, Germany) kit. For the collection, an endocervical brush was introduced 4cm into the anal canal, rotated five times, removed, agitated in the transport solution (PapilloCheck® collection medium) provided by the manufacturer and then the brush tip left in the fluid . The resulting cell suspension was stored dry at 4–8°C.…”

Section: Methodsmentioning

confidence: 99%

A comparative study between conventional and liquid‐based cytology in screening for anal intraepithelial lesions in HIV‐positive patients

Maia

Marinho

Barbosa

et al. 2014

Diagnostic Cytopathology

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Anal intraepithelial neoplasia (AIN) is associated with HPV infection and can be detected by cytological screening. While conventional exfoliative cytology (CC) is a low-cost and nonaggressive method, liquid-based cytology (LBC) tends to give clearer readings. Although studies of the efficacy of anal cancer screening methods would be of great importance for groups at high risk for AIN, few such studies have been conducted. The aim of the present study was to assess the concordance of CC and LBC in diagnosing anal pre-neoplastic lesions, and to compare cytological results with anoscopy, histopathological, and molecular biology findings. Comparative study involving 33 HIV-positive patients, who underwent anoscopy and biopsy of suspected lesions. Concordance between the two cytology methods was calculated, as were the associations between cytology results and findings from other screening methods. A total of 54.5% of cases were considered AIN-negative by CC and LBC, and concordance between the two methods was statistically significant (P < 0.05). Anoscopy was negative in 15 of the 18 CC- and LBC-negative cases. CC identified 75% of patients with positive biopsy, while LBC identified 85.71% of these patients. Molecular biology results showed that patients with LSIL tested positive for the highest number of HPV subtypes. The associations between positive biopsy and high grade HPV, HPV 16, and multiple HPV infections were not statistically significant. Conventional and liquid-based cytology are equally effective in screening for anal preneoplastic lesions.

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Section: Methodsmentioning

confidence: 99%

A comparative study between conventional and liquid‐based cytology in screening for anal intraepithelial lesions in HIV‐positive patients

Maia

Marinho

Barbosa

et al. 2014

Diagnostic Cytopathology

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show abstract

“…Endocervical brushes were used to collect anal samples. For the collection, the technique used was the introduction of the brush 4 cm into the anal canal and rotation three to four times . Brushes were then agitated in a transport solution provided by the manufacturer and the resulting cell suspension was stored dry at 4–8°C.…”

Section: Methodsmentioning

confidence: 99%

HPV load and anal cytological abnormalities

et al. 2015

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“…The technique used for collection consisted of introducing the brush 4 cm into the anal canal and rotating it. [8] The slides were fixed in alcohol and stained using the Papanicolaou method. Cytological abnormalities were classified according to the 2001 Bethesda criteria: Negative for malignancy; ASC-US; low grade squamous intraepithelial lesion; ASC - cannot exclude a high-grade lesion; and high-grade squamous intraepithelial lesion and invasive carcinoma.…”

Section: Methodsmentioning

confidence: 99%

Value of human papillomavirus typing for detection of anal cytological abnormalities

Maia

Marinho

Barbosa

et al. 2013

Indian J Sex Transm Dis

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Purpose:The objective of this study was to evaluate anal cytology and human papillomavirus (HPV) typing in patients with human immunodeficiency virus infection.Materials and Methods:Anal samples were collected from 61 patients (44 men and 17 women) and analyzed by PapilloCheck test and conventional cytology.Results:Of all anal samples, 37.7% had cytological abnormalities, 47.54% were negative and 14.75% were unsatisfactory. High-risk HPV, multiple high-risk HPV and HPV 16 infection was detected in 91.13%, 78.26% and 47.82% of the samples with cytological abnormalities and in 47.54%, 6.89% and 3.44% of the negative samples, respectively. High-risk HPV infection was significantly more frequent in anal samples with cytological abnormalities than in negative samples (P = 0.0005, Fisher's test), particularly multiple high-risk HPV infection (P < 0.0001) and HPV 16 infection (P = 0.0002).Conclusions:High-risk HPV, multiple high-risk HPV and HPV 16 infections are significantly associated with anal cytological abnormalities. Furthermore, the frequency of HPV infection in anal cytological samples suggests that high-risk HPV detection has high sensitivity, but low specificity for detection of anal cytological abnormalities, but multiple high-risk HPV typing and HPV 16 typing have a lower sensitivity and high specificity. Results suggest that HPV typing may be useful as an adjunct to cytology to screen patients for high-resolution anoscopy and biopsy.

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Quanto a escova deve ser introduzida no canal anal para avaliação citológica mais eficaz?

Cited by 16 publications

References 30 publications

A comparative study between conventional and liquid‐based cytology in screening for anal intraepithelial lesions in HIV‐positive patients

A comparative study between conventional and liquid‐based cytology in screening for anal intraepithelial lesions in HIV‐positive patients

HPV load and anal cytological abnormalities

Value of human papillomavirus typing for detection of anal cytological abnormalities

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