Quantitative trait locus analysis of spot blotch and leaf rust resistance in the BCD47 × Baronesse barley mapping population

Abstract

We studied the genetics of the resistance to leaf rust (LR) (caused by Puccinia hordei) and spot blotch (SB) (caused by Cochliobolus sativus) in barley using a doubled-haploid population derived from the cross BCD47 · Baronesse. BCD47 has low SB severity and high susceptibility to LR, while Baronesse is susceptible to SB and has low LR severity. Resistance to both diseases is expressed at the adult plant stage. The population was phenotyped in eight field environments for SB and nine for LR. Ten quantitative trait loci (QTLs) were detected for SB. None were significant in more than three environments, and both parents contributed resistance alleles. Five QTLs were detected for LR. The most consistent quantitative trait locus (QTL) (significant in seven environments) was on chromosome 6H (located on the Bmag173-Bmag009 interval) with Baronesse contributing the resistance allele. Coincident QTL effects for SB were also detected in this region with resistance alleles to the two diseases in repulsion. These results illustrate the difficulties of resistance gene detection in the complex disease environments found under field conditions.

Effects of n-butanol on barley microspore embryogenesis

“…Additionally, Barley DHs have been of great value to speed up the development of mapping populations for marker-trait associations and comparative genomics and map-based cloning Pickering 2005, Forster et al 2007). Recently DHs have played a fundamental role in the application of high-throughput genotyping systems in the construction of barley consensus maps (Alsop et al 2010, Muñoz-Amatriaín et al 2011, Poland et al 2012, and use of marker assisted selection (MAS) systems (Melchinger et al 2011) especially for resistance to disease and abiotic stress (Riedel et al 2011, Castro et al 2012, Fisk et al 2013). …”

Evaluating Barley for the Emerging Craft Malting Industry in Western Washington

“…Bob and Richard both have Baronesse in their pedigree and have a similar level of resistance to leaf rust. Full Pint carries a single major leaf rust resistance gene, allelic to Rph3 (Castro et al, 2012). Harrington was rated as resistant to powdery mildew when it was released (Harvey and Rossnagel, 1984) and Richard was found to be resistant in the present study.…”

Characterization of Leaf Rust Resistance in International Barley Germplasm Using Genome-Wide Association Studies

“…MTA1 and MTA2 were detected at positions 331.01 and 482.85 Mbp on chromosome 1H and shown to be in linkage disequilibrium with Rph20 -linked MTA. Several previous studies have also reported markers linked to QTLs in the area between 388.13 and 505.86 Mbp [ 15 , 25 , 33 , 34 ]. The putative QTLs MTA3 and MTA4 are located in the same region as Rph8/14/15/16 and are in linkage disequilibrium, which indicates that they are inherited together.…”