Quantitative trait loci (QTL) mapping for physiological and biochemical attributes in a Pasban90/Frontana recombinant inbred lines (RILs) population of wheat (Triticum aestivum) under salt stress condition

Abstract

Salt stress causes nutritional imbalance and ion toxicity which affects wheat growth and production. A population of recombinant inbred lines (RILs) were developed by crossing Pasban90 (salt tolerant) and Frontana (salt suceptible) for identification of quantitative trait loci (QTLs) for physiological traits including relative water content, membrane stability index, water potential, osmotic potential, total chlorophyll content, chlorophyll a, chlorophyll b and biochemical traits including proline contents, superoxide dismutase, sodium content, potassium content, chloride content and sodium/potassium ratio by tagging 202 polymorphic simple sequence repeats (SSR) markers. Linkage map of RILs comprised of 21 linkage group covering A, B and D genome for tagging and maped a total of 60 QTLs with major and minor effect. B genome contributed to the highest number of QTLs under salt stress condition. Xgwm70 and Xbarc361 mapped on chromosome 6B was linked with Total chlorophyll, water potential and sodium content. The increasing allele for all these QTLs were advanced from parent Pasban90. Current study showed that Genome B and D had more potentially active genes conferring plant tolerance against salinity stress which may be exploited for marker assisted selection to breed salinity tolerant high yielding wheat varieties.

Kinetin mitigates Cd-induced damagesto growth, photosynthesis and PS II photochemistry of Trigonella seedlings by up-regulating ascorbate-glutathione cycle

“…Cytokinins (CKs) for example, take part in numerous physiological activities such as cell division and morphogenesis, flower and seed development and chloroplast development [12][13][14][15][16]. CKs can also regulate the abiotic stresses by the up-regulation of nitrogen metabolism and antioxidant defense system of plants [12,[17][18][19][20][21]; the importance of CKs in the regulation of heavy metal stress tolerance in plants is well known [11,17,18,22], but the mechanism of CKs induced regulation of heavy metal stress tolerance needs further investigation. The work reported here aimed to determine the effect of exogenous application of different level of KN (KN L , KN M and KN H ) on Trigonella seedlings (in order to counteract Cd phyto-toxicity) by examining their effects on growth and photochemistry of the PS II and AsA-GSH cycles.…”

Using Exogenous Melatonin, Glutathione, Proline, and Glycine Betaine Treatments to Combat Abiotic Stresses in Crops

“…External applications of proline can maintain the turgidity of the cell under stress, protect plants from harmful radiations, increase photosynthetic and transpiration rate, stomatal conductance, and antioxidants, thus increasing the growth and yield of plants even when under abiotic stresses. However, the excessive applications of proline can also impart toxic effects on plants [ 100 ].…”

General Outlook of Identified Genomic Regions/QTLs Associated with Salt Tolerance Traits in Wheat (Triticum aestivum L.)

“…These genes encoded for high-affinity K transporter (HKT) proteins; HKT1;4 (Nax1) and HKT 1;5 (Nax2) and their possible role were in regulating the Na+ transportation from root to shoot [39]. Last few years, QTL mapping has been applied for identification of QTLs/genomic regions in wheat for salt tolerance associated traits such as chlorophyll, seedling biomass, plant dry weights, grain yield, spikelet number, germination, tiller number and leaf injury, Na + and K + concentration and Na + /K + ratio in shoots [40][41][42][43][44][45][46][47][48][49][50][51][52][53]. GWAS applied on a diverse panel of wheat cultivars genotyped using 90K SNP array, 35K SNP array and 660K SNP array for mapping of salt tolerance traits in the seedling hydroponics and adult field condition [42,43,53].…”