Quantitative Trait Loci Controlling Lodging Resistance and other Important Agronomic Traits in Dry Field Peas

Smitchger, Jamin A.; Weeden, N. F.

doi:10.2135/cropsci2018.04.0260

Cited by 15 publications

(16 citation statements)

References 87 publications

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“…If genes for climbing ability and yield had been linked to the afila locus, it would have been unlikely that the backcross progeny could recover the full climbing ability and pod yield of the recurrent parent varieties, which are vigorous climbers. Linkage drag was not observed with the afila gene in our analyzed cross, but has been seen in lodging studies with afila by Smitchger and Weeden (2019) [43]. Climbing ability and its effects on disease and lodging tolerance would likely be an additive trait with complex inheritance and quantitative trait loci (QTL).…”

Section: Utility Of the Afila Gene For Introgression In Climbing Peassupporting

confidence: 52%

Introgression of the Afila Gene into Climbing Garden Pea (Pisum sativum L.)

Checa

Rodriguez

et al. 2020

Agronomy

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The pea (Pisum sativum L.) is one of the most important crops in temperate agriculture around the world. In the tropics, highland production is also common with multiple harvests of nearly mature seeds from climbing plant types on trellises. While the leafless variant caused by the afila gene is widely used in developing row-cropped field peas in Europe, its use for trellised garden peas has not been reported. In this study we describe a pea breeding program for a high-elevation tropical environment in the Department of Nariño in Colombia, where over 16,000 hectares of the crop are produced. The most widespread climbing varieties in the region are ‘Andina’ and ‘Sindamanoy’, both of which have high-biomass architecture with abundant foliage. They are prone to many diseases, but preferred by farmers given their long production season. This plant type is expensive to trellis, with wooden posts and plastic strings used for vine staking constituting 52% of production costs. The afila trait could reduce these costs by creating interlocking plants as they do in field peas. Therefore, our goal for this research was to develop a rapid breeding method to introduce the recessive afila gene, which replaces leaves with tendrils, into the two commercial varieties used as recurrent parents (RPs) with three donor parents (DPs)—‘Dove’, ‘ILS3575′ and ‘ILS3568′—and to measure the effect on plant height (PH) and yield potential. Our hypothesis was that the afila gene would not cause linkage drag while obtaining a leafless climbing pea variety. Backcrossing was conducted without selfing for two generations and plants were selected to recover recurrent parent characteristics. Chi-square tests showed a ratio of 15 normal leaved to one afila leaved in the BC2F2 plants, and 31:1 in the BC2F3 generation. Selecting in the last of these generations permitted a discovery of tall climbing plants that were similar to those preferred commercially, but with the stable leafless afila. The method saved two seasons compared to the traditional method of progeny testing before each backcross cycle; the peas reached the BC2F2 generation in five seasons and the BC3F2 in seven seasons. This is advantageous with trellised peas that normally require half a year to reach maturity. Leafless garden peas containing the afila gene were of the same height as recurrent parents and, by the third backcross, were equally productive, without the high biomass found in the traditional donor varieties. The value of the afila gene and the direct backcrossing scheme is discussed in terms of garden pea improvement and crop breeding.

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Section: Utility Of the Afila Gene For Introgression In Climbing Peassupporting

confidence: 52%

Introgression of the Afila Gene into Climbing Garden Pea (Pisum sativum L.)

Checa

Rodriguez

et al. 2020

Agronomy

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“…Major QTLs explaining 58% (Tar'an et al, 2003), 50% (Smitchger 2017), and >30% of phenotypic variance for lodging resistance were identified in bi-parental mapping populations (Gali et al, 2018). Ferrari et al (2016) identified QTLs for lodging resistance on LG3 and LG4.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Mapping for Agronomic and Seed Quality Traits of Field Pea (Pisum sativum L.)

et al. 2019

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Genome-wide association study (GWAS) was conducted to identify loci associated with agronomic (days to flowering, days to maturity, plant height, seed yield and seed weight), seed morphology (shape and dimpling), and seed quality (protein, starch, and fiber concentrations) traits of field pea (Pisum sativum L.). A collection of 135 pea accessions from 23 different breeding programs in Africa (Ethiopia), Asia (India), Australia, Europe (Belarus, Czech Republic, Denmark, France, Lithuania, Netherlands, Russia, Sweden, Ukraine and United Kingdom), and North America (Canada and USA), was used for the GWAS. The accessions were genotyped using genotyping-by-sequencing (GBS). After filtering for a minimum read depth of five, and minor allele frequency of 0.05, 16,877 high quality SNPs were selected to determine marker-trait associations (MTA). The LD decay (LD1/2max,90) across the chromosomes varied from 20 to 80 kb. Population structure analysis grouped the accessions into nine subpopulations. The accessions were evaluated in multi-year, multi-location trials in Olomouc (Czech Republic), Fargo, North Dakota (USA), and Rosthern and Sutherland, Saskatchewan (Canada) from 2013 to 2017. Each trait was phenotyped in at least five location-years. MTAs that were consistent across multiple trials were identified. Chr5LG3_566189651 and Chr5LG3_572899434 for plant height, Chr2LG1_409403647 for lodging resistance, Chr1LG6_57305683 and Chr1LG6_366513463 for grain yield, Chr1LG6_176606388, Chr2LG1_457185, Chr3LG5_234519042 and Chr7LG7_8229439 for seed starch concentration, and Chr3LG5_194530376 for seed protein concentration were identified from different locations and years. This research identified SNP markers associated with important traits in pea that have potential for marker-assisted selection towards rapid cultivar improvement.

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“…cultivars with an improved harvest index and resistance to lodging (Święcicki & Surma, 2002). Factors such as wind and rain can cause mechanical damage, particularly of the basal, thin internodes of the stem (Smitchger, 2017). Canopy lodging causes reduced sunlight penetration and creates a microclimate that favors development of fungal diseases (Zhang et al., 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Mendel's height gene ( Le ) influences internode length, with semi‐dwarf lines being less susceptible to lodging. Smitchger (2017) indicated that the semi‐dwarf mutation ( le ) was the most important genetic factor influencing lodging resistance in pea. A decreased susceptibility for lodging in pea has been achieved by breeding semi‐dwarf cultivars with the afila (semi‐leafless) mutation.…”

Section: Introductionmentioning

confidence: 99%

Quantitative trait loci for stem strength properties and lodging in two pea biparental mapping populations

et al. 2021

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Lodging is the major factor that determines whether pea (Pisum sativum L.) can be mechanically harvested. The aim of this experiment was to investigate the relationship between stem mechanical parameters (diameter, stem wall thickness, stiffness [rigidity], and maximum self-weight moment [stem strength]) and lodging in pea.Three years of data on stem mechanical parameters and 2 yr of data on lodging resistance were collected on the 'Carneval' × 'MP1401' pea cultivars and Wt10245 × Wt11238 lines. Mechanical parameters were measured separately for three stem sections: lower, middle, and upper. Nine quantitative trait loci (QTL) associated with stem mechanical parameters were identified in the Carneval × MP1401 genetic map. Eight QTL associated with stem mechanical parameters were identified in the Wt10245 × Wt11238 genetic map. These results confirmed QTL for lodging resistance found previously, especially in regards to located in chr5LG3 close to the A004 marker. Quantitative trait loci for stem diameter and stem strength of the upper and lower stem colocated with the Rfs marker in chr5LG3. A plant height QTL was detected close to AB141 marker, 2 cM (3.3 Mbp) from the Le gene. These results enable identification of pea genomic regions associated with agriculturally important traits and provide a foundation for further research to improve lodging resistance in pea.

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Quantitative Trait Loci Controlling Lodging Resistance and other Important Agronomic Traits in Dry Field Peas

Cited by 15 publications

References 87 publications

Introgression of the Afila Gene into Climbing Garden Pea (Pisum sativum L.)

Introgression of the Afila Gene into Climbing Garden Pea (Pisum sativum L.)

Genome-Wide Association Mapping for Agronomic and Seed Quality Traits of Field Pea (Pisum sativum L.)

Quantitative trait loci for stem strength properties and lodging in two pea biparental mapping populations

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