Quantitative study of free amino acids in human eccrine sweat excreted from the forearms of healthy trained and untrained men during exercise

Abstract

The free amino acids in eccrine sweat collected from the forearms of 20 healthy trained and 20 healthy untrained men during controlled exercise were determined quantitatively using ion exchange column chromatography. Sweat was deproteinized by adding an equal volume of 5% sulphosalicylic acid. The amino acid concentrations showed a constant qualitative pattern in sweat and large individual differences. Essential amino acids, such as isoleucine, leucine, lysine, methionine, phenylalanine, and valine were excreted in relatively small amounts. As compared to the trained men, untrained men showed statistically significantly higher concentrations in sweat for the following amino acids: Alanine, arginine, glycine, histidine, isoleucine, leucine, lysine, ornithine, phenylalanine, serine, taurine, threonine, tyrosine, and valine. No significant differences were found for citrulline, cystine, ethanolamine, and methionine. The comparison of the amino acid excretions in sweat obtained under controlled exercise and in urine showed that the amounts of amino acids excreted in sweat under controlled exercise were comparable to the losses of amino acids in urine.

Study of metabolite composition of eccrine sweat from healthy male and female human subjects by 1H NMR spectroscopy

“…The amino acid concentrations of sweat collected during exercise being two to eighteen times higher than those observed during thermal stimulation. The amino acid profile obtained in the current study is in general agreement with previous studies (Liappis et al, 1979), that reported free amino acid concentrations exhibited a constant qualitative pattern but large quantitative differences between subjects. Interestingly, a previous study reported proline to be only present in eccrine sweat collected from females (Liappis et al, 1980) however, the results of the current study do not support this finding.…”

Urinary arginine and ornithine in occupational exposure to 2-ethylhexanoic acid

“…It seems to be dose dependent in man. Small amounts of citrulline are excreted in the urine of healthy controls (Liappis et al 1979). We found none in our nine subjects.…”

“…Similarly, the hepatic urea cycle can be probed by analysis for nonprotein amino acids in the urine (Batshaw et al 1982). Specifically, urinary arginine and orOffprint requests to: H. Savolainen, IUMHT, rue du Bugnon 19, CH-1005 Lausanne, Switzerland nithine concentrations are not much affected by muscle mass (Liappis et al 1979) so that, e. g. the training status or work load should not interfere with the analysis.…”

Oxidative Properties of Skin Exudates – A Determinant for Nickel Diffusion: A Review

“…Total average AA values collected from 20 trained men and 20 untrained men were 12,797 and 24,855 Ìmol/l, respectively. The highest values were seen for serine (3,954/7,782), glycine (2,239/4,392), alanine (1,556/3,028) and threonine (1,057/1,856) for trained versus untrained men, respectively [71].…”