Quantitative Simultaneous Multianalyte Detection of DNA by Dual‐Wavelength Surface‐Enhanced Resonance Raman Scattering

Abstract

SERRS gut: Die quantitative Detektion von fünf markierten Oligonucleotiden gelingt durch das im Titel genannte Verfahren SERRS, ohne dass eine Trennung erforderlich ist. Die Empfindlichkeit der Mehrfachanalyse ist dabei die gleiche wie beim Nachweis der einzelnen Farbstoffe.

Enzyme-free detection and quantification of double-stranded nucleic acids

“…Consequently several SERRS-labeled oligonucleotides can be simultaneously detected if the absorption maxima of the tags are close enough to the laser excitation wavelength [25][26][27][28][29][30]. Maximum absorption of HEX and R6G is at 535 and 524 nm, respectively, and can therefore be simultaneously measured with an argon laser tuned at 514.5 nm [27,29,31,37]. Moreover, their Raman signals enable specific discrimination of each probe, because of its most intense peaks centered at 1632 cm −1 and 1650 cm −1 for HEX and R6G, respectively (Fig.…”

“…Several SERRS-labeled oligonucleotides can be simultaneously detected [25][26][27][28][29][30]. A variety of molecules can be detected by SERRS if their absorption maxima are close enough to the laser excitation wavelength.…”

“…The fluorescence of the SERRS-label is quenched by the metallic surface enabling the amplified Raman fingerprint of the molecule to be observed. A wide range of labels can be detected by SERRS, and the sharpness of Raman peaks gives SERRS high multiplexing capacity [25][26][27][28][29][30]. SERRS has been developed for DNA detection since 1997 [24,31] and has become a promising enzyme-free tool for specific detection and quantification of single-stranded DNA on either micro-chips [30,32,33] or magnetic micro-beads [34,35].…”

Quantitative DNA Analysis Using Surface‐Enhanced Resonance Raman Scattering

“…Between 2001 and 2003, the first examples of a SERRS DNA 2-plex, using rhodamine-and HEX-labelled oligonucleotides [48], and a 3-plex, using FAM, TET and Cy3, were developed by Graham and co-workers [49]. Faulds et al demonstrated the first 5-plex system where FAM-, Cy5.5-, BODIPY-, R6G-and ROX-labelled oligonucleotides were detected in situ as shown in Figure 11.5 [50]. In the study, the oligonucleotide mixture was maintained at a concentration of 1.82 × 10 −9 mol dm −3 throughout.…”

Single‐Molecule‐ and Trace Detection by SERS

“…It has been recognized that the SERRS cross sections for common fluorophores are similar to fluorescence and has the additional advantages of possessing much higher information content: the spectrum contains many characteristic vibrational peaks [31]. These possible advantages for SERRS have been identified, and the exploration of the potential of SERRS for multiplexing applications is an active area of research [32,33].…”