Quantitative DNA Analysis Using Surface‐Enhanced Resonance Raman Scattering

Faulds, Karen; Stevenson, Ross; Graham, Duncan

doi:10.1002/9783527632756.ch11

Cited by 3 publications

(4 citation statements)

References 65 publications

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“…A rapid change of color was also observed in the case of a film with MO (Movie S3). These preliminary results demonstrate that DNA–chitosan films with dyes hold much promise for developing photonic and sensing materials. , …”

Section: Resultsmentioning

confidence: 73%

“…These preliminary results demonstrate that DNA−chitosan films with dyes hold much promise for developing photonic and sensing materials. 52,53 ■ CONCLUSIONS Aerogels have been prepared from homogeneous hydrogels that were fabricated using a recently reported approach, which provided the bottom-up coassembly of native ds-DNA with chitosan without common phase separation (coacervation). Aerogels turned into regenerated hydrogels through a process of rehydration.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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DNA–Chitosan Aerogels and Regenerated Hydrogels with Extraordinary Mechanical Properties

Postnova

Sarin

Zinchenko

et al. 2021

ACS Appl. Polym. Mater.

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It has recently been shown that native ds-DNA and chitosan can coassemble without common phase separation and precipitation, forming a translucent homogeneous hydrogel. This simple approach consists of progressive charging of the polysaccharide through gradual acidification of a solution in the presence of DNA, which slowly strengthens the electrostatic interactions between them, ruling out fast and uncontrolled cooperative association, which is a well-known problem associated with coacervation. Homogeneous hydrogels thus fabricated demonstrated mechanical properties characteristic of quite strong physical hydrogels. Extraordinary mechanical strength and elasticity have been observed in aerogels prepared from an original hydrogel and also in hydrogels, which were restored by the rehydration of aerogels. Aerogels match highly cross-linked synthetic polyurethane foams despite DNA with chitosan being bound only via electrostatic interactions without using a toxic cross-linking agent. Owing to their biocompatibility, biodegradability, and available renewable resources, hydrogels and aerogels might replace typical synthetic polymers. The fabrication of bionanocomposites with luminescent, sensing, and photocatalytic functionalities by entrapping quantum dots, titania nanoparticles, and dyes demonstrates some possible applications.

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Section: Resultsmentioning

confidence: 73%

Section: ■ Results and Discussionmentioning

confidence: 99%

DNA–Chitosan Aerogels and Regenerated Hydrogels with Extraordinary Mechanical Properties

Postnova

Sarin

Zinchenko

et al. 2021

ACS Appl. Polym. Mater.

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“…The simultaneous quantifi cation of several differently labeled SERS tags has provided an improved selectivity over conventional fl uorescence -based assays [24,25] .…”

Section: Surface -Enhanced Raman Spectroscopy ( Sers )mentioning

confidence: 99%

Surface Raman Spectroscopy

Hill¹,

Lendl

2011

Surface and Thin Film Analysis

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“…While this delivered high sensitivities, it also rendered these approaches not label free. Despite the use of labels, the narrow vibrational Raman peaks enabled a high degree of spectral multiplexing, by enabling ease of resolving presence of a large population of molecules in a single spot [50]. Such spectral multiplexing, although can be performed also with fluorescence is limited to typically up to 4, and further requires careful choice of molecules with well-separated fluorescence peaks.…”

Section: Sensingmentioning

confidence: 99%

New opportunities for nanotechnology in the field of diagnostics based on proteomics

García¹,

Krishnamoorthy²

2019

Front Nanosci Nanotech

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New developments in personalised medicine pose a technological challenge for the implementation of diagnostic devices demanding an increase for the speed, throughput and sensitivity for the detection of proteomic biomarkers. Following the tracks of genetics where the progress came through the analysis of DNA fragments, peptides, which are small fragments of proteins, can provide a new tool to get insights into the analysis of macromolecules. However, the current methods of combinatorial chemistry and sensing based on liquid handling and labelling, respectively, cannot cope with the demands of throughput and speed required by clinical applications. Nanotechnology holds promises to improve in-situ synthesis of microarrays and label free sensing to provide the combinatorial flexibility, quickness and sensitivity to help for the implementation of proteomic diagnosis in precision medicine.

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Quantitative DNA Analysis Using Surface‐Enhanced Resonance Raman Scattering

Cited by 3 publications

References 65 publications

DNA–Chitosan Aerogels and Regenerated Hydrogels with Extraordinary Mechanical Properties

DNA–Chitosan Aerogels and Regenerated Hydrogels with Extraordinary Mechanical Properties

Surface Raman Spectroscopy

New opportunities for nanotechnology in the field of diagnostics based on proteomics

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