Quantitative screening of human stratum corneum lipids by sequential one‐dimensional thin layer chromatography

Abstract

There is a need in many studies on the epidermal permeability barrier for practical and reliable separation of all major fractions of human stratum corneum lipids. Various methods have been described including thin layer chromatography, high performance thin layer chromatography and iatroscan. However, none of these methods seems to be applicable for an inexpensive, rapid and reliable analysis of a large number of samples. Here, such a method for the separation and quantification of all major stratum corneum lipid fractions is presented. This method employs the one-dimensional separation of stratum corneum lipids using thin layer chromatography. The systems used herein are based upon various modifications of the solvents, solvent ratio and developing distance of each system. For quantification of chromatographed and charred lipids a Desaga densitometer is used. The system described here had been successfully applied to 550 samples of human stratum corneum lipids.

Lipids

“…Bonte et al (145) separated stratum corneum lipids by automated multiple development (AMD) on HPTLC silica gel plates using an initial isocratic step followed by a 25-step gradient from methanol-water to hexane. Schuerer et al (146) used densitometric quantification for the separation and analysis of human stratum corneum lipids by sequential one-dimensional TLC with detection by charring. Watanabe and Mizuta (147) detected glycosphingolipids from biological samples by TLC at the 5 pmol level using 5-hydroxy-l-tetralone as fluorescent labeling reagent.…”

Contribution to Liquid Chromatographic Analysis of Cutaneous Ceramides

“…Although HPTLC 9 is the most used, other analytical methods have been used, like HPLC, [10][11] GC/MS, 12 FTIR, 13 NMR. Although HPTLC 9 is the most used, other analytical methods have been used, like HPLC, [10][11] GC/MS, 12 FTIR, 13 NMR.…”